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Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga
Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311) qPCR Master Mixes Biomiga

Biomiga 2X SYBR Green qPCR Master Mix, No ROX, Hot Start (QP1311)

$110.00

    Biomiga's SB-Green 2X qPCR Master Mix is a ready-to-use SYBR Green I mix with everything except primers and template: antibody-inactivated hot-start Taq, dNTPs, Mg²⁺, enhancer and stabilizers, with no ROX reference dye.

    • No ROX: for Bio-Rad CFX96/CFX384/CFX Connect, Rotor-Gene, Illumina Eco, Roche LightCycler 480 and Eppendorf realplex (per Biomiga)
    • Hot start activates at 95 °C; standard and fast cycling protocols supplied
    • 100 or 500 reactions of 50 µL

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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2X
Ready-to-use mix
Hot start
Antibody-inactivated Taq
3 mM
Final Mg²⁺ at 1X
No ROX
Reference dye

SYBR GREEN qPCR · NO ROX

Add primers and template. Everything else is in the mix.

Biomiga's SB-Green 2X qPCR Master Mix (QP1311) is a ready-to-use SYBR Green I master mix for real-time quantitative PCR. It contains hot-start Taq DNA polymerase, dNTPs, Mg²⁺, SYBR Green I dye, enhancer and stabilizers. It contains no internal reference dye, for real-time instruments that do not require ROX. The hot-start Taq is antibody-inactivated, which blocks polymerase activity at room temperature; activity is restored once the reaction reaches 95 °C.

For research use only. Not for use in diagnostic procedures.

How it works

SYBR Green qPCR workflow with standard and fast cycling conditions for Biomiga 2X qPCR master mixes

Biomiga's suggested starting protocol. Optimal conditions vary with primer set.

STEP 1

Thaw and mix

Thaw at room temperature, mix gently, spin down and keep on ice.

STEP 2

Assemble

Combine the 2X mix, primers, template and water on ice or at room temperature, then seal with optical caps or film and spin down.

STEP 3

Activate and cycle

95 °C for 2 min, then 30–45 cycles of 95 °C and 60 °C, collecting data at 60 °C.

STEP 4

Melt curve

Biomiga recommends a melt curve on every run to check for primer dimers and confirm specificity.

Reaction setup

Component 50 µL reaction 20 µL reaction Final
2X qPCR Mix (No ROX) 25 µL 10 µL 1X
Forward primer (10 µM) 1.0 µL 0.4 µL 200 nM (100–500 nM)
Reverse primer (10 µM) 1.0 µL 0.4 µL 200 nM (100–500 nM)
Template variable variable —
Nuclease-free water to 50 µL to 20 µL —

Reactions scale from 5 to 50 µL; keep all volumes proportional. Adding template in 5–10 µL improves precision, and undiluted cDNA should be no more than 10% of the reaction.

Cycling conditions

Step Standard cycling Fast cycling
Taq activation 95 °C, 2 min 95 °C, 2 min
Denature (30–45 cycles) 95 °C, 10–15 s 95 °C, 3–5 s
Anneal/extend + data collection 60 °C, 30–60 s 60 °C, 20–30 s
Melt curve Per instrument Per instrument

Template input

cDNA from 1 pg–100 ng of total RNA; genomic DNA 100 pg–100 ng; plasmid DNA 10–10⁷ copies.

Primers

Design for 70–150 bp amplicons. 200 nM per primer suits most reactions; titrate between 100 and 500 nM if needed.

Magnesium

3 mM final works for most targets; the optimum can be 3–6 mM. Add 1, 2 or 3 µL of 50 mM MgCl₂ per 50 µL to reach 4, 5 or 6 mM (MgCl₂ not included).

Which ROX version fits my instrument?

Which Biomiga SYBR Green qPCR mix fits your instrument: QP1311 No ROX highlighted

Same SYBR Green chemistry; only the ROX reference dye differs. Compatibility per Biomiga.

Mix ROX Compatible instruments (per Biomiga)
QP1311 (this mix) No ROX Bio-Rad CFX96, CFX384 and CFX Connect; Corbett Research Rotor-Gene Q, 3000 and 6000; Illumina Eco; Roche LightCycler 480; Eppendorf Mastercycler ep realplex
QP1312 Low ROX Applied Biosystems 7500, 7500 Fast and ViiA 7; Stratagene Mx3000P, Mx3005P and Mx4000; Agilent AriaMx
QP1313 ROX Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT; StepOne and StepOnePlus

Not sure? Check your instrument's passive reference (ROX) setting, or email info@bluetigerscientific.com or text (833) 928-4437.

LOW ROX

Biomiga SYBR Green qPCR Mix, Low ROX (QP1312)

For Applied Biosystems 7500, 7500 Fast and ViiA 7 and more.

ROX

Biomiga SYBR Green qPCR Mix, ROX (QP1313)

For Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT and more.

BEFORE YOU START

You supply

Primers, template and nuclease-free water; optical plates or tubes and sealing film; optional 50 mM MgCl₂. From our store: 96-well qPCR plates, qPCR tubes and 8-strips and optical sealing films.

In the kit

Pack 2X mix
100 reactions 2 × 1.25 mL
500 reactions 10 × 1.25 mL

Each 2X mix contains hot-start Taq, dNTPs, 6 mM Mg²⁺ (3 mM at 1X), SYBR Green I, enhancer and stabilizers.

Built for

Gene expression

Two-step RT-qPCR after cDNA synthesis.

DNA quantification

Genomic and plasmid targets on a standard curve.

Core and teaching labs

One ready-to-use mix across users and plates.

When this mix is the right call

  • Your instrument matches: Bio-Rad CFX96, CFX384 and CFX Connect and the other systems listed above.
  • All-in-one: add only primers, template and water.
  • Fast cycling: Biomiga supplies a fast protocol with 3–5 s denaturation.
  • Forgiving storage: Biomiga reports no significant performance loss after 10 freeze-thaw cycles.

Related Biomiga products

Cell & Tissue RNA Mini Kit (R6311)

Total RNA from cells and tissue for RT-qPCR.

Cell/Tissue RNA Kit Plus (R6321)

Adds a gDNA clearance column before qPCR.

Express Hi Fi DNA Polymerase (EHF1101)

High-fidelity PCR for cloning standards and inserts.

Also from Biomiga: 1st cDNA Synthesis Kit (FS01) and Probe-based 2X qPCR Master Mix (QP1314). Email info@bluetigerscientific.com or text (833) 928-4437 for a quote.

Frequently asked questions

Quick facts

What it is: A 2X ready-to-use SYBR Green I qPCR master mix with hot-start Taq (No ROX).

Instruments: Bio-Rad CFX96, CFX384 and CFX Connect; Corbett Research Rotor-Gene Q, 3000 and 6000; Illumina Eco; Roche LightCycler 480; Eppendorf Mastercycler ep realplex.

Packs: 100 reactions (2 × 1.25 mL) or 500 reactions (10 × 1.25 mL).

Storage: −20 °C, protected from light.

Use: In vitro research use only.

Which version do I need?

Match the mix to your instrument's ROX requirement. QP1311 (No ROX) is for Bio-Rad CFX96, CFX384 and CFX Connect; Corbett Research Rotor-Gene Q, 3000 and 6000; Illumina Eco; Roche LightCycler 480; Eppendorf Mastercycler ep realplex. If your instrument isn't listed, check its passive reference setting or ask us.

Can I use this mix with TaqMan or other probes?

No. These mixes use SYBR Green I dye-based detection. For probe assays, ask us about Biomiga's Probe-based 2X qPCR Master Mix (QP1314).

What do I need to add?

Primers, template and nuclease-free water. Add 50 mM MgCl₂ only if you want more than the built-in 3 mM final.

Does it support fast cycling?

Yes. After the 2-minute activation at 95 °C, Biomiga's fast protocol uses 95 °C for 3–5 s and 60 °C for 20–30 s per cycle.

How should it be stored?

At −20 °C, protected from light. For convenience it can be kept at 4 °C for up to 6 months. Avoid repeated freeze-thaw, although Biomiga reports no significant performance loss after 10 cycles.

What amplicon size works best?

70–150 bp. Biomiga advises keeping amplicons under 500 bp and running a melt curve on every plate to check for primer dimers.

How is the mix qualified?

Biomiga functionally tests the mix in qPCR and reports linear resolution over six orders of magnitude (R² > 0.995) with efficiency above 90%.

How many reactions are in a pack?

100 reactions (2 × 1.25 mL) or 500 reactions (10 × 1.25 mL) of 2X mix, sized for 50 µL reactions. Reactions can be scaled down to 5 µL, which stretches each pack further.

Can I switch from another vendor's SYBR Green mix?

Usually, as long as the ROX level matches your instrument. Biomiga has not published side-by-side data, so confirm primer efficiency on a standard curve before switching an established assay.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

SYBR is a trademark of Thermo Fisher Scientific. Instrument names are trademarks of their respective owners. Blue Tiger Scientific is not affiliated with these companies.

    SpecificationDetail
    ManufacturerBiomiga, Inc. (San Diego, CA)
    Catalog NumberQP1311-01 (100 reactions), QP1311-02 (500 reactions)
    Format2X ready-to-use SYBR Green I qPCR master mix
    ContainsAntibody-inactivated hot-start Taq DNA polymerase, dNTPs, 6 mM Mg²⁺ (3 mM final at 1X), SYBR Green I dye, enhancer, stabilizers
    ROX Reference DyeNo ROX
    Compatible Instruments (per Biomiga)Bio-Rad CFX96, CFX384 and CFX Connect; Corbett Research Rotor-Gene Q, 3000 and 6000; Illumina Eco; Roche LightCycler 480; Eppendorf Mastercycler ep realplex
    Pack ContentsQP1311-01: 2 × 1.25 mL (100 reactions of 50 µL); QP1311-02: 10 × 1.25 mL (500 reactions of 50 µL)
    Reaction Volume5–50 µL
    Activation95 °C, 2 min
    QualificationFunctionally tested in qPCR; linear resolution over six orders of magnitude (R² > 0.995), efficiency > 90% (Biomiga-stated)
    Storage−20 °C, protected from light; may be kept at 4 °C for up to 6 months; avoid repeated freeze-thaw
    ShippingShips frozen
    Country of OriginUSA
    HazardousConsult the current manufacturer SDS and supplied instructions for buffer hazards, handling, and storage.
    Intended UseIn vitro research use only (RUO)

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

    Payment Terms
    Orders are prepaid by card, or invoiced against an institutional purchase order once credit is approved. Prices are in USD and subject to change without notice. Purchase orders are due within 30 days of invoice unless agreed otherwise in writing.

    Order Confirmation & Cancellations
    Confirmed orders are placed with the manufacturer and are final. Changes or cancellations must be requested in writing before shipment and approved by Blue Tiger Scientific; approved cancellations may incur restocking, materials or third-party fees.

    Accuracy of Orders
    Customers are responsible for verifying catalog numbers, pack sizes, ship-to address and recipient availability before submitting an order.

    Shipping & Cold Chain
    Shipping is calculated at checkout. Temperature-sensitive products ship by expedited service, and ship dates may be adjusted to avoid weekend or holiday transit. Orders placed on weekends or holidays are processed the next business day. Delivery dates are estimates and are not guaranteed. A recipient must be available to receive and store temperature-sensitive products on arrival. Blue Tiger Scientific is not liable for carrier delays, weather disruptions or other events beyond our control.

    Receiving & Claims
    Inspect every shipment on arrival. Shortages, damage or thawed temperature-sensitive product must be reported to Blue Tiger Scientific in writing, with photos, within 2 business days of delivery. Claims received after that window, and shipments that are refused, unclaimed or left unattended, are not eligible for credit or replacement.

    Returns & Refunds
    Returns require a Return Merchandise Authorization (RMA) issued by Blue Tiger Scientific and must be requested within 30 days of delivery. Refunds or replacements are issued only for shipping errors or verified product defects. Temperature-sensitive products are not returnable once delivered. Customers pay return shipping unless the error was ours, and refunds exclude original shipping charges. Approved refunds are processed within 45 business days.

    Warranty
    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

    Limitation of Liability
    Blue Tiger Scientific's total liability for any claim is limited to the purchase price of the product involved. Blue Tiger Scientific and Biomiga are not liable for indirect, incidental or consequential damages, including lost research time or materials, arising from the use or misuse of any product.

    Safety
    Handle all products according to the Safety Data Sheet and the manufacturer's instructions, using appropriate personal protective equipment.

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    Customers must use products in compliance with all applicable local, state, federal and international laws, including export control and biosafety regulations. Products may not be exported or re-exported without the required authorizations.

    Resale
    Products may not be resold or redistributed without prior written approval from Blue Tiger Scientific.

    Force Majeure
    Neither party is liable for delays or failures caused by events beyond its reasonable control, including weather, carrier disruptions, supply shortages or changes in regulation.

    Governing Law
    These terms are governed by the laws of the State of California and the United States.

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