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Biomiga Cell/Tissue Total RNA Extraction Kit Plus, R6321
R6321 key specs: under 30 minutes Biomiga-stated prep time, 50–100 µL elution, 30 mg tissue or 1 × 10⁷ cells max, 4, 50 or 250 preps
R6321 workflow: lyse in Buffer HLY, remove genomic DNA on the DNA clearance column, bind and elute on the ezBind RNA column, purified RNA for NGS, RT-PCR, qPCR and microarray
Kit chooser: R6321 Plus with gDNA clearance columns and ezBind RNA columns versus R6311 with RNA columns and optional DNase I
R6321 components for 4, 50 and 250 preps: Buffer HLY, Buffer HRB, RNA Wash Buffer, DEPC-treated water, ezBind RNA columns, DNA clearance columns
Biomiga Cell/Tissue Total RNA Extraction Kit Plus, R6321
R6321 key specs: under 30 minutes Biomiga-stated prep time, 50–100 µL elution, 30 mg tissue or 1 × 10⁷ cells max, 4, 50 or 250 preps
R6321 workflow: lyse in Buffer HLY, remove genomic DNA on the DNA clearance column, bind and elute on the ezBind RNA column, purified RNA for NGS, RT-PCR, qPCR and microarray
Kit chooser: R6321 Plus with gDNA clearance columns and ezBind RNA columns versus R6311 with RNA columns and optional DNase I
R6321 components for 4, 50 and 250 preps: Buffer HLY, Buffer HRB, RNA Wash Buffer, DEPC-treated water, ezBind RNA columns, DNA clearance columns

Biomiga Cell/Tissue Total RNA Extraction Kit Plus, gDNA Removal Columns (R6321)

$20.00

    Purify total RNA from cultured cells and animal tissue with genomic DNA removed on a dedicated clearance column before RNA binding. Eluted in 50–100 µL of RNase-free water, ready for NGS, RT-PCR, qPCR and microarray work.

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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<30 min
Biomiga-stated prep time
50–100 µL
Elution volume
30 mg
Max tissue per prep (or 1 × 10⁷ cells)
4 / 50 / 250
Preps per kit

TOTAL RNA WITH gDNA REMOVAL

Genomic DNA cleared before the RNA ever reaches the column.

The Biomiga Cell/Tissue Total RNA Extraction Kit Plus (R6321) lyses samples in denaturing Buffer HLY, which Biomiga says immediately inhibits RNase activity. The lysate passes through a DNA Clearance column that traps genomic DNA. The flow-through is mixed with ethanol, bound to an ezBind RNA column, washed twice, and eluted in 50–100 µL of DEPC-treated water. Biomiga states the protocol takes under 30 minutes and describes the RNA as ready for NGS, RT-PCR, qPCR, differential display and microarrays.

For research use only. Not for use in diagnostic procedures.

How it works

R6321 Plus overview: DNA clearance, transfer of RNA-containing flow-through, RNA capture and purification

Workflow overview; follow the R6321 user manual for volumes, speeds and buffer preparation.

STEP 1

Lyse

Add 500 µL Buffer HLY (with β-mercaptoethanol added) and homogenize. Use 700 µL for more than 5 × 10⁶ cells or more than 15 mg tissue.

STEP 2

Clear gDNA

Spin the lysate through the DNA Clearance column. Keep the flow-through; the genomic DNA stays on the column.

STEP 3

Bind and wash

Add half a volume of 100% ethanol, bind to the ezBind RNA column, then wash with Buffer HRB and RNA Wash Buffer. Finish with a dry spin.

STEP 4

Elute

Elute in 50–100 µL of DEPC-treated water. Biomiga notes that re-applying the eluate typically recovers another 20–30% of the RNA.

Full protocol, volumes and speeds are in the user manual.

Input and expected yield

Biomiga's reported total RNA yields per 10 mg of tissue or per 10⁶ cells. Yields vary with sample type, quality and handling.

Sample Input Total RNA yield (µg)
Liver 10 mg 50
Heart 10 mg 50
Spleen 10 mg 30–40
Kidney 10 mg 20–30
Muscle 10 mg 20
Pancreas 10 mg 20
Lung 10 mg 10–20
COS-7 cells 1 × 10⁶ 30
HeLa cells 1 × 10⁶ 15
HEK293 / NIH 3T3 cells 1 × 10⁶ 10–12

Don't exceed 30 mg of tissue or 1 × 10⁷ cells per column; overloading lowers yield and lets genomic DNA through.

What to expect from the RNA

Size range

RNA shorter than about 200 bases doesn't bind efficiently, so this kit isn't suited to miRNA or other small-RNA work.

Genomic DNA

Biomiga states that the clearance column and RNA column together remove nearly all genomic DNA, so DNase digestion isn't needed for most applications. Sensitive applications may need an additional DNase I digest; Biomiga's DNase I (E11-01) is sold separately.

Getting the most RNA

Do

  • Add β-mercaptoethanol (or 1 M DTT) to the Buffer HLY you'll use, as the manual describes; it then keeps up to 1 month at room temperature
  • Warm Buffer HLY to 37 °C if crystals form
  • Add ethanol to the RNA Wash Buffer before first use
  • Remove all culture medium before lysis
  • Stabilize tissue in RNAsecure solution or snap-freeze it

Don't

  • Exceed 30 mg tissue or 1 × 10⁷ cells per column
  • Skip the dry spin before elution
  • Add bleach or acidic solutions to the preparation waste; Biomiga's manual notes Buffer HLY contains chaotropic salts

BEFORE YOU START

You supply

100% ethanol, β-mercaptoethanol (or 1 M DTT), a microcentrifuge, RNase-free 1.5 mL tubes and tips, and a homogenizer for tissue samples.

What's in the box

Component 4 50 250 preps
Buffer HLY 4 mL 40 mL 150 mL
Buffer HRB 4 mL 50 mL 150 mL
RNA Wash Buffer (concentrate) 2 mL 12 mL 50 mL
DEPC-treated water 1 mL 10 mL 30 mL
ezBind RNA columns 4 50 250
DNA Clearance columns 4 50 250

Biomiga also lists collection tubes.

Built for

NGS input

Biomiga lists NGS among the applications for this RNA.

RT-qPCR

gDNA cleared on a column, without a separate DNase step for most assays.

Cell-line panels

Yield guidance for HeLa, HEK293, NIH 3T3 and COS-7.

Tissue work

Input guidance for seven animal tissues.

Which Biomiga RNA kit do I need?

R6321 dedicated DNA-clearance column compared with R6311 optional-DNase workflow; sensitive R6321 applications may also require DNase

Documented kit contents differ; we don't compare yields between kits.

THIS KIT

Cell/Tissue RNA Kit Plus (R6321)

Total RNA with genomic DNA removed on a clearance column.

Cell & Tissue RNA Mini (R6311)

No gDNA column; an optional DNase step instead.

96-well Tissue RNA Kit (R6811)

Tissue RNA in a 96-well plate format.

RNAsecure Solution (R1011)

Stabilize RNA in harvested tissue before extraction.

Need one of the others? Email info@bluetigerscientific.com or text (833) 928-4437 for a quote.

When this kit is the right call

  • No DNase step for most work: genomic DNA is removed on the clearance column; add DNase only for sensitive applications.
  • Ready for NGS and qPCR: applications listed by Biomiga for the purified RNA.
  • Cell-line guidance: expected yields for four common lines.
  • Low-risk trial: 4 preps to test your own samples before committing to 50 or 250.

Frequently asked questions

Quick facts

What it is: A silica spin-column kit for total RNA from cultured cells and animal tissue, with a genomic DNA clearance column.

Elution: 50–100 µL DEPC-treated water.

Max input: 30 mg tissue or 1 × 10⁷ cells per prep.

Pack sizes: 4, 50 and 250 preps.

Use: In vitro research use only.

How is genomic DNA removed?

The lysate passes through a DNA Clearance column that traps genomic DNA before the RNA binds to the ezBind RNA column. Biomiga notes the clearance column also helps homogenize the sample.

Do I still need a DNase step?

Usually not. Biomiga states the clearance column and RNA column together remove nearly all genomic DNA, so DNase digestion isn't necessary for most downstream applications. Some sensitive applications may need an additional DNase I digest; Biomiga's DNase I (E11-01) is sold separately.

Does it recover miRNA or other small RNAs?

No. Biomiga notes that RNA shorter than about 200 bases doesn't bind the column efficiently, so this kit isn't suited to small-RNA work.

What samples can I use, and how much?

Cultured cells (suspension or adherent) and animal tissue. Use 500 µL Buffer HLY for up to 5 × 10⁶ cells or 15 mg tissue, and 700 µL for up to 1 × 10⁷ cells or 30 mg tissue. Don't exceed 1 × 10⁷ cells or 30 mg tissue per column; overloading causes genomic DNA carryover.

What do I need to prepare before first use?

Add β-mercaptoethanol (or 1 M DTT) to the volume of Buffer HLY you'll use, as described in the user manual; once added, Buffer HLY keeps at room temperature for up to 1 month. Add 100% ethanol to the RNA Wash Buffer: 8 mL for the 4-prep kit, 48 mL for the 50-prep kit, or 200 mL for the 250-prep kit. If crystals form in Buffer HLY, dissolve them at 37 °C before use.

How long does a prep take?

Biomiga states the protocol can be completed in under 30 minutes. Tissue homogenization time varies by sample.

How should the kit be stored and handled?

All components store at room temperature. Store purified RNA at −20 °C or colder. The expiry date is printed on the label. Biomiga's manual advises gloves and eye protection when handling Buffers HLY and HRB, and not adding bleach or acidic solutions to the preparation waste.

How does it compare with the QIAGEN RNeasy Plus Mini Kit?

Both are silica spin-column kits that remove genomic DNA on a dedicated column before RNA binding. Both retain RNA longer than about 200 nucleotides, and both list a maximum of 1 × 10⁷ cells or 30 mg tissue per prep. QIAGEN publishes up to 100 µg total RNA per RNeasy Plus Mini column and a protocol under 25 minutes. Biomiga states under 30 minutes for R6321 and doesn't publish a column capacity, so we don't compare capacity or yield. Buffers and protocols differ, so validate on your own samples; the 4-prep trial is sized for that. RNeasy and QIAGEN are trademarks of QIAGEN; Blue Tiger Scientific is not affiliated with QIAGEN.

Can this kit be used for clinical or diagnostic work?

No. It is intended for in vitro research use only and is not for use in diagnostic procedures or in humans.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

    SpecificationDetail
    Catalog NumbersR6321-00 (4 preps), R6321-01 (50 preps), R6321-02 (250 preps)
    FormatDNA Clearance column plus ezBind RNA spin column, microcentrifuge protocol
    Sample TypesCultured cells; animal tissue
    Max Input1 × 10⁷ cells or 30 mg tissue per prep
    RNA RetainedRNA longer than about 200 nt
    Genomic DNARemoved on the DNA Clearance column; optional DNase I digest for sensitive applications (not included)
    Elution50–100 µL DEPC-treated water
    Prep TimeUnder 30 minutes (Biomiga-stated)
    User-Supplied100% ethanol, β-mercaptoethanol or 1 M DTT, RNase-free 1.5 mL tubes
    StorageRoom temperature; Buffer HLY with β-ME or DTT keeps up to 1 month at room temperature
    Shelf LifeSee label
    Country of OriginUSA
    HazardsConsult the current manufacturer SDS and supplied instructions for buffer hazards, handling, and storage.
    Intended UseIn vitro research use only (RUO); not for use in humans

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

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    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

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    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

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