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Biomiga Cell & Tissue Total RNA Extraction Kit, R6311
R6311 key specs: about 30 minutes Biomiga-stated prep time, 50–100 µL elution, RNA over 200 nt retained, 4, 50 or 250 preps
R6311 workflow overview: cell or tissue RNA preparation, spin-column purification, purified RNA for RT-PCR, real-time PCR, Northern and microarray
Kit chooser: R6311 with RNA columns and optional DNase I versus R6321 Plus with gDNA clearance columns
R6311 components for 50-prep and 250-prep kits: Buffer LY, Buffer RB, RNA Wash Buffer, DEPC-treated water, RNA columns
Biomiga R6311 Cell & Tissue RNA Mini Kit, additional product view
Biomiga Cell & Tissue Total RNA Extraction Kit, R6311
R6311 key specs: about 30 minutes Biomiga-stated prep time, 50–100 µL elution, RNA over 200 nt retained, 4, 50 or 250 preps
R6311 workflow overview: cell or tissue RNA preparation, spin-column purification, purified RNA for RT-PCR, real-time PCR, Northern and microarray
Kit chooser: R6311 with RNA columns and optional DNase I versus R6321 Plus with gDNA clearance columns
R6311 components for 50-prep and 250-prep kits: Buffer LY, Buffer RB, RNA Wash Buffer, DEPC-treated water, RNA columns
Biomiga R6311 Cell & Tissue RNA Mini Kit, additional product view

Biomiga Cell & Tissue Total RNA Extraction Kit, Spin Column (R6311)

$20.00

    Purify total RNA from cultured cells and animal tissue on a silica spin column, eluted in 50–100 µL of RNase-free water for RT-PCR, real-time PCR, Northern and microarray work. Start with the 4-prep trial size.

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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All products are covered by manufacturer warranty

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After-Sale Support

24/7 support

~30 min
Biomiga-stated prep time
50–100 µL
Elution volume
>200 nt
RNA retained by the column
4 / 50 / 250
Preps per kit

TOTAL RNA, CELLS AND TISSUE

Total RNA from cells and tissue in a single spin-column run.

The Biomiga Cell & Tissue Total RNA Extraction Kit (R6311) isolates total RNA from cultured cells and animal tissue without phenol/chloroform extraction. Samples are lysed in Buffer LY, bound to an RNA column with ethanol, washed, and eluted in 50–100 µL of DEPC-treated water. Biomiga describes the RNA as ready for RT-PCR, real-time PCR, Northern analysis and microarray work, and especially suited to quantitative gene-expression studies.

For research use only. Not for use in diagnostic procedures.

How it works

R6311 workflow overview: cell or tissue RNA preparation, spin-column purification, purified RNA for RT-PCR, real-time PCR, Northern and microarray

Workflow overview; follow the R6311 user manual for volumes, speeds and incubation times.

STEP 1

Lyse

Add 500 µL Buffer LY (with β-mercaptoethanol added) to the cell pellet or well. Homogenize tissue in Buffer LY with a rotor-stator or ultrasonic homogenizer.

STEP 2

Bind

Add half a volume of 100% ethanol to the lysate, then spin it through the RNA column.

STEP 3

Wash

Wash with Buffer RB, then RNA Wash Buffer. An optional on-column DNase I step fits in between. Finish with a dry spin, lid open.

STEP 4

Elute

Add 50–100 µL of DEPC-treated water and spin. Store the RNA at −20 °C.

Full protocol, volumes and speeds are in the user manual.

Input and expected yield

Biomiga's recommended maximum per prep, with reported total RNA yields. Yields vary with sample quality and handling.

Sample Max per prep Total RNA yield (µg)
Liver 30 mg 130
Pancreas 30 mg 100
Spleen 15 mg 80
Brain 120 mg 80
Lung 100 mg 70
Heart 100 mg 50
Muscle 80 mg 50
Kidney 20 mg 45
Tomato leaves 200 mg 30
HeLa cells 5 × 10⁶ 120

Using more tissue than recommended can exceed the column's capacity, which lowers yield and raises genomic DNA carryover.

What to expect from the RNA

Size range

RNA shorter than about 200 bases doesn't bind efficiently, so this kit isn't suited to miRNA or other small-RNA work.

Genomic DNA

Biomiga notes that only trace genomic DNA remains. The optional on-column DNase I step removes it when your assay needs that. DNase I is not included; Biomiga sells it separately.

Getting the most RNA

Do

  • Add 1% β-mercaptoethanol to Buffer LY before use, then keep it at 4 °C
  • Add ethanol to the RNA Wash Buffer before first use
  • Snap-freeze tissue you can't process right away
  • Check RNA integrity on a denaturing gel before critical downstream work

Don't

  • Exceed the input in the table above
  • Skip the lid-open dry spin
  • Load excess RNA into RT-PCR if genomic DNA signal is a problem; Biomiga suggests 50–100 ng

BEFORE YOU START

You supply

100% ethanol, β-mercaptoethanol, a microcentrifuge, a tissue homogenizer (for tissue samples), RNase-free DNase I (optional), and pipettes with RNase-free filter tips.

What's in the box

Component 50 preps 250 preps
Buffer LY 30 mL 135 mL
Buffer RB 30 mL 135 mL
RNA Wash Buffer (concentrate) 12 mL 50 mL
DEPC-treated water 10 mL 30 mL
RNA columns 50 250

Biomiga also lists collection tubes and 1.5 mL RNase-free microfuge tubes. The 4-prep trial contains the same components in trial quantities.

Built for

Gene expression

RT-qPCR from cell and tissue samples.

Tissue panels

Input guidance for ten sample types.

Northern and microarray

Total RNA in a small elution volume.

Method switching

Validate on your own samples with the 4-prep trial.

Which Biomiga RNA kit do I need?

Kit chooser: R6311 with RNA columns and optional DNase I versus R6321 Plus with gDNA clearance columns

Documented kit contents differ; we don't compare yields between kits.

THIS KIT

Cell & Tissue RNA Mini (R6311)

Total RNA, with an optional DNase step for gDNA.

Cell/Tissue RNA Kit Plus (R6321)

Adds a gDNA clearance column to remove genomic DNA without the DNase step.

96-well Tissue RNA Kit (R6811)

The same job in a 96-well plate format.

1st cDNA Synthesis Kit (FS01)

The next step after extraction.

Need one of the others? Email info@bluetigerscientific.com or text (833) 928-4437 for a quote.

When this kit is the right call

  • Routine total RNA: cells and animal tissue for RT-qPCR, Northern or microarray.
  • gDNA under your control: trace levels by default, with an on-column DNase step when needed.
  • Simple storage: kit components store at room temperature.
  • Low-risk trial: 4 preps to test your own samples before committing to 50 or 250.

Frequently asked questions

Quick facts

What it is: A silica spin-column kit for total RNA from cultured cells and animal tissue.

Elution: 50–100 µL DEPC-treated water.

RNA size: RNA longer than about 200 nt is retained.

Pack sizes: 4, 50 and 250 preps.

Use: In vitro research use only.

Does it recover miRNA or other small RNAs?

No. Biomiga notes that RNA shorter than about 200 bases doesn't bind the column efficiently, so this kit isn't suited to small-RNA work.

Is the RNA free of genomic DNA?

Not guaranteed. Biomiga notes trace genomic DNA may remain. You can add the optional on-column DNase I step (DNase sold separately), or choose R6321 Plus, which includes a gDNA clearance column.

What sample types does it handle?

Suspension and adherent cultured cells, and animal tissues such as liver, kidney, spleen, lung, heart, muscle, brain and pancreas. Biomiga's yield table also includes tomato leaves. Ask us before using other plant tissues.

What do I need to prepare before first use?

Add 1% β-mercaptoethanol to Buffer LY and store it at 4 °C. Add 100% ethanol to the RNA Wash Buffer: 48 mL for the 50-prep kit, 200 mL for the 250-prep kit.

How long does a prep take?

Biomiga describes the method as taking about 30 minutes. The optional DNase step adds a 15-minute incubation, and tissue homogenization time varies by sample.

How should the kit be stored?

Kit components store at room temperature. Once β-mercaptoethanol is added, keep Buffer LY at 4 °C. Store purified RNA at −20 °C or colder. The expiry date is printed on the label.

How does it compare with the QIAGEN RNeasy Mini Kit?

Both are silica spin-column kits for total RNA from cells and animal tissue, and both are designed around RNA longer than about 200 nucleotides, so neither is a small-RNA kit. Neither removes genomic DNA in its standard protocol without an optional DNase step. QIAGEN publishes a binding capacity of up to 100 µg per RNeasy Mini column; Biomiga's R6311 manual doesn't state a column capacity, so we don't compare capacity or yield. The two kits use different buffers and protocols, so validate R6311 on your own samples first; the 4-prep trial pack is sized for that. RNeasy and QIAGEN are trademarks of QIAGEN; Blue Tiger Scientific is not affiliated with QIAGEN.

Can this kit be used for clinical or diagnostic work?

No. It is intended for in vitro research use only and is not for use in diagnostic procedures or in humans.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

    SpecificationDetail
    Catalog NumbersR6311-00 (4 preps), R6311-01 (50 preps), R6311-02 (250 preps)
    FormatSilica spin column, microcentrifuge protocol
    Sample TypesCultured cells; animal tissue (see input table)
    Max Input (examples)5 × 10⁶ HeLa cells; 30 mg liver; up to 120 mg brain
    RNA RetainedRNA longer than about 200 nt
    Genomic DNATrace levels; optional on-column DNase I (not included)
    Elution50–100 µL DEPC-treated water
    Prep TimeAbout 30 minutes (Biomiga-stated)
    User-Supplied100% ethanol, β-mercaptoethanol
    StorageRoom temperature; Buffer LY at 4 °C after β-ME is added
    Shelf LifeSee label
    Country of OriginUSA
    HazardsConsult the current manufacturer SDS and supplied instructions for buffer hazards, handling, and storage.
    Intended UseIn vitro research use only (RUO); not for use in humans

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

    Payment Terms
    Orders are prepaid by card, or invoiced against an institutional purchase order once credit is approved. Prices are in USD and subject to change without notice. Purchase orders are due within 30 days of invoice unless agreed otherwise in writing.

    Order Confirmation & Cancellations
    Confirmed orders are placed with the manufacturer and are final. Changes or cancellations must be requested in writing before shipment and approved by Blue Tiger Scientific; approved cancellations may incur restocking, materials or third-party fees.

    Accuracy of Orders
    Customers are responsible for verifying catalog numbers, pack sizes, ship-to address and recipient availability before submitting an order.

    Shipping & Cold Chain
    Shipping is calculated at checkout. Temperature-sensitive products ship by expedited service, and ship dates may be adjusted to avoid weekend or holiday transit. Orders placed on weekends or holidays are processed the next business day. Delivery dates are estimates and are not guaranteed. A recipient must be available to receive and store temperature-sensitive products on arrival. Blue Tiger Scientific is not liable for carrier delays, weather disruptions or other events beyond our control.

    Receiving & Claims
    Inspect every shipment on arrival. Shortages, damage or thawed temperature-sensitive product must be reported to Blue Tiger Scientific in writing, with photos, within 2 business days of delivery. Claims received after that window, and shipments that are refused, unclaimed or left unattended, are not eligible for credit or replacement.

    Returns & Refunds
    Returns require a Return Merchandise Authorization (RMA) issued by Blue Tiger Scientific and must be requested within 30 days of delivery. Refunds or replacements are issued only for shipping errors or verified product defects. Temperature-sensitive products are not returnable once delivered. Customers pay return shipping unless the error was ours, and refunds exclude original shipping charges. Approved refunds are processed within 45 business days.

    Warranty
    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

    Limitation of Liability
    Blue Tiger Scientific's total liability for any claim is limited to the purchase price of the product involved. Blue Tiger Scientific and Biomiga are not liable for indirect, incidental or consequential damages, including lost research time or materials, arising from the use or misuse of any product.

    Safety
    Handle all products according to the Safety Data Sheet and the manufacturer's instructions, using appropriate personal protective equipment.

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    Customers must use products in compliance with all applicable local, state, federal and international laws, including export control and biosafety regulations. Products may not be exported or re-exported without the required authorizations.

    Resale
    Products may not be resold or redistributed without prior written approval from Blue Tiger Scientific.

    Force Majeure
    Neither party is liable for delays or failures caused by events beyond its reasonable control, including weather, carrier disruptions, supply shortages or changes in regulation.

    Governing Law
    These terms are governed by the laws of the State of California and the United States.

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