Skip to content

Global shipping available. Please request a quote to receive customized pricing for your region.

Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01)
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01)
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga
Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01) cDNA Synthesis Kits Biomiga

Biomiga 1st Strand cDNA Synthesis Kit, Oligo(dT) & Hexamer (FS01)

$40.00

    Biomiga's 1st Strand cDNA Synthesis Kit converts total or poly(A)+ RNA into first-strand cDNA for PCR and real-time qPCR. Its reverse transcriptase mix combines an engineered MMLV RT with a ribonuclease inhibitor, and both oligo(dT)20 and random hexamer annealing buffers are included.

    • 10 pg to 5 µg total RNA per 20 µL reaction
    • Oligo(dT)20 and random hexamer primers both supplied
    • Biomiga states RNA targets from 100 bp to 10 kb can be detected

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

See All Specs

aeroplane.png__PID:119d4b1a-2623-400c-8fa4-aec5b49a3bf0

Fast Delivery

Most items ship same day

protection.png__PID:4b1a2623-e00c-4fa4-aec5-b49a3bf05f92

Manufacturer Warranty

All products are covered by manufacturer warranty

technical-support.png__PID:e00c8fa4-aec5-449a-bbf0-5f923431936b

After-Sale Support

24/7 support

10 pg–5 µg
Total RNA input
20 µL
Reaction volume
dT + hexamer
Primers included
2 years
Stability at −20 °C

TWO-STEP RT-PCR · cDNA SYNTHESIS

From RNA to PCR-ready cDNA in about an hour.

Biomiga's 1st Strand cDNA Synthesis Kit (FS01) converts total or poly(A)+ RNA into first-strand cDNA. Its reverse transcriptase mix combines an engineered MMLV reverse transcriptase with a ribonuclease inhibitor protein, and the kit supplies both oligo(dT)20 and random hexamer annealing buffers. Biomiga states the system works from 10 pg to 5 µg of total RNA and detects RNA targets from 100 bp to 10 kb, and that it is suited to gene isolation, high-throughput expression profiling by real-time quantitation, and end-point RT-PCR.

For research use only. Not for use in diagnostic procedures.

How it works

First-strand cDNA synthesis workflow for Biomiga FS01: anneal primer at 65 °C, add RT mix, incubate at 37 °C for 50 min, use 1–2 µL in PCR or qPCR

Biomiga's 1st-strand protocol, 20 µL per reaction.

STEP 1

Anneal primer

Combine RNA with 5 µL annealing buffer OT (oligo(dT)20) or RH (random hexamer), water to 10 µL. Heat at 65 °C for 3 min, then place on ice.

STEP 2

Add RT mix

Add 10 µL of reaction mixture (9 µL cDNA synthesis buffer + 1 µL RT and RNase inhibitor mix). Mix gently and spin briefly.

STEP 3

Reverse transcribe

37 °C for 50 min. With random hexamers, incubate 5 min at room temperature (22–24 °C) first.

STEP 4

Use or store

Optionally heat to 85 °C for 5 min. Use 1–2 µL of the 20 µL reaction per PCR; store the rest at −20 °C.

Reaction setup

RNA/primer mix (10 µL) Oligo(dT)20 Random hexamer
RNA (10 pg–5 µg) x µL x µL
Annealing buffer OT 5.0 µL —
Annealing buffer RH — 5.0 µL
Nuclease-free water to 10 µL to 10 µL
RT reaction mixture 1 reaction 10 reactions
cDNA synthesis buffer 9.0 µL 90 µL
RT and RNase inhibitor mix 1.0 µL 10 µL
Total 10 µL 100 µL

For mRNA targets under 1.5 kb, a 30-minute incubation is an option. RT temperature can be optimized between 37 and 42 °C. Heat inactivation at 85 °C is recommended when using less than 10 ng of RNA.

No-RT control

For accurate expression profiling, run the same amount of RNA without reverse transcriptase. Any signal in that control points to genomic DNA contamination.

DNase option

If the no-RT control shows product, Biomiga describes an optional DNase I treatment before cDNA synthesis. DNase I and its buffer are not included.

Which kit do I need?

Two-step or one-step: Biomiga FS01 1st Strand cDNA Synthesis Kit highlighted, compared with the RT0201 One-Step RT-PCR Kit

Both kits start from purified RNA; they differ in how the cDNA is made and used.

THIS KIT

1st Strand cDNA Synthesis Kit (FS01)

Makes a reusable cDNA pool with oligo(dT) or random hexamer priming, for qPCR or end-point PCR.

ONE-STEP

One-Step RT-PCR Kit (RT0201)

RT and PCR in one tube with your own primers; end-point product for gel analysis.

For quantitative results, run FS01 cDNA with a Biomiga SYBR Green qPCR mix matched to your instrument: no ROX (QP1311), low ROX (QP1312) or ROX (QP1313).

BEFORE YOU START

You supply

Purified RNA (for example from the R6311 or R6321 kits), PCR or qPCR reagents for the next step, and optional RNase-free DNase I.

In the box

Component 20 rxn 50 rxn
RT and RNase inhibitor mix 20 µL 50 µL
cDNA synthesis buffer 250 µL 500 µL
Annealing buffer OT (oligo(dT)20) 125 µL 250 µL
Annealing buffer RH (random hexamer) 125 µL 250 µL
Nuclease-free water 1.0 mL 1.5 mL

The 5- and 100-reaction packs contain the same five components in 5- and 100-reaction quantities.

When this kit is the right call

  • Many targets, one RNA sample: make a cDNA pool once and run as many PCR or qPCR assays as you need
  • Your choice of priming: oligo(dT)20 for poly(A) mRNA or random hexamers for broader coverage
  • Wide input range: 10 pg to 5 µg total RNA per reaction, per Biomiga
  • Trial size: the 5-reaction pack lets you test it on your own samples

Related Biomiga products

Cell & Tissue RNA Mini Kit (R6311)

Total RNA from cells and tissue.

Cell/Tissue RNA Kit Plus (R6321)

Adds a gDNA clearance column.

SYBR Green qPCR Master Mixes (QP1311–QP1313)

Quantify your cDNA by real-time PCR.

Express Hi Fi DNA Polymerase (EHF1101)

High-fidelity PCR from cDNA for cloning.

Frequently asked questions

Quick facts

What it is: A first-strand cDNA synthesis system with an engineered MMLV RT, RNase inhibitor, and oligo(dT)20 and random hexamer primers

RNA input: 10 pg–5 µg total RNA per 20 µL reaction

Packs: 5, 20, 50 or 100 reactions

Storage: −20 °C (non-frost-free); stable 2 years

Use: In vitro research use only

Should I use oligo(dT) or random hexamers?

Oligo(dT)20 primes from the poly(A) tail of mRNA. Random hexamers prime throughout the RNA, including transcripts without a poly(A) tail. Both annealing buffers are included, so you can choose per experiment.

How much RNA can I use?

From 10 pg to 5 µg of total RNA per 20 µL reaction, per Biomiga. Poly(A)+ purified RNA can also be used.

How long does it take?

About an hour: 3 minutes at 65 °C to anneal, then 50 minutes at 37 °C (plus 5 minutes at room temperature first with random hexamers). For mRNA targets under 1.5 kb, 30 minutes at 37 °C is an option.

How much cDNA goes into a PCR?

Biomiga recommends 1–2 µL of the 20 µL first-strand reaction per PCR. Store the rest at −20 °C.

Do I need to heat-inactivate the RT?

It is optional. Biomiga recommends 85 °C for 5 minutes when the reaction used less than 10 ng of RNA.

How do I check for genomic DNA contamination?

Run a no-RT control with the same amount of RNA but no reverse transcriptase. If it gives a signal, treat the RNA with RNase-free DNase I before cDNA synthesis, or start from an RNA kit with gDNA removal such as R6321.

Is DNase I included?

No. Biomiga describes an optional DNase I step, but DNase I and its reaction buffer are supplied by you.

Should I choose this kit or the One-Step RT-PCR Kit?

Choose FS01 when you want a cDNA pool to use in several assays or in qPCR. Choose RT0201 when you want to go from RNA to an end-point PCR product in one tube.

Can I switch from another vendor's cDNA synthesis kit?

Usually, but Biomiga has not published side-by-side data. Run a small pilot with a no-RT control before switching an established assay.

How should the kit be stored?

At −20 °C in a non-frost-free freezer. Biomiga states the kit is stable for 2 years when stored this way.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

Instrument and product names are trademarks of their respective owners.

    SpecificationDetail
    ManufacturerBiomiga, Inc. (San Diego, CA)
    Catalog NumberFS01-00 (5 reactions), FS01-01 (20 reactions), FS01-02 (50 reactions), FS01-03 (100 reactions)
    FormatFirst-strand cDNA synthesis system, 20 µL reactions
    Reverse TranscriptaseMixture of engineered MMLV RT and ribonuclease inhibitor protein
    PrimersOligo(dT)20 (annealing buffer OT) and random hexamer (annealing buffer RH)
    RNA Input10 pg–5 µg total RNA, or poly(A)+ RNA
    Targets100 bp–10 kb (Biomiga-stated)
    Protocol65 °C 3 min anneal; 37 °C 50 min (optimizable 37–42 °C); optional 85 °C 5 min inactivation
    Contents (20 / 50 rxn)RT and RNase inhibitor mix 20 / 50 µL; cDNA synthesis buffer 250 / 500 µL; annealing buffer OT 125 / 250 µL; annealing buffer RH 125 / 250 µL; nuclease-free water 1.0 / 1.5 mL
    Contents (5 / 100 rxn)Same five components in 5- and 100-reaction quantities
    Not IncludedRNA template; DNase I (optional); PCR or qPCR reagents
    QualificationFunctionally tested for amplification of a 1.1 kb RNA target from 10 ng total RNA (Biomiga-stated)
    Storage−20 °C (non-frost-free); stable for 2 years
    ShippingShips frozen
    Country of OriginUSA
    HazardousConsult the current manufacturer SDS and supplied instructions for buffer hazards, handling, and storage.
    Intended UseIn vitro research use only (RUO)

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

    Payment Terms
    Orders are prepaid by card, or invoiced against an institutional purchase order once credit is approved. Prices are in USD and subject to change without notice. Purchase orders are due within 30 days of invoice unless agreed otherwise in writing.

    Order Confirmation & Cancellations
    Confirmed orders are placed with the manufacturer and are final. Changes or cancellations must be requested in writing before shipment and approved by Blue Tiger Scientific; approved cancellations may incur restocking, materials or third-party fees.

    Accuracy of Orders
    Customers are responsible for verifying catalog numbers, pack sizes, ship-to address and recipient availability before submitting an order.

    Shipping & Cold Chain
    Shipping is calculated at checkout. Temperature-sensitive products ship by expedited service, and ship dates may be adjusted to avoid weekend or holiday transit. Orders placed on weekends or holidays are processed the next business day. Delivery dates are estimates and are not guaranteed. A recipient must be available to receive and store temperature-sensitive products on arrival. Blue Tiger Scientific is not liable for carrier delays, weather disruptions or other events beyond our control.

    Receiving & Claims
    Inspect every shipment on arrival. Shortages, damage or thawed temperature-sensitive product must be reported to Blue Tiger Scientific in writing, with photos, within 2 business days of delivery. Claims received after that window, and shipments that are refused, unclaimed or left unattended, are not eligible for credit or replacement.

    Returns & Refunds
    Returns require a Return Merchandise Authorization (RMA) issued by Blue Tiger Scientific and must be requested within 30 days of delivery. Refunds or replacements are issued only for shipping errors or verified product defects. Temperature-sensitive products are not returnable once delivered. Customers pay return shipping unless the error was ours, and refunds exclude original shipping charges. Approved refunds are processed within 45 business days.

    Warranty
    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

    Limitation of Liability
    Blue Tiger Scientific's total liability for any claim is limited to the purchase price of the product involved. Blue Tiger Scientific and Biomiga are not liable for indirect, incidental or consequential damages, including lost research time or materials, arising from the use or misuse of any product.

    Safety
    Handle all products according to the Safety Data Sheet and the manufacturer's instructions, using appropriate personal protective equipment.

    Legal & Export Compliance
    Customers must use products in compliance with all applicable local, state, federal and international laws, including export control and biosafety regulations. Products may not be exported or re-exported without the required authorizations.

    Resale
    Products may not be resold or redistributed without prior written approval from Blue Tiger Scientific.

    Force Majeure
    Neither party is liable for delays or failures caused by events beyond its reasonable control, including weather, carrier disruptions, supply shortages or changes in regulation.

    Governing Law
    These terms are governed by the laws of the State of California and the United States.

Questions about a product? Ask us here.

email us here: info@bluetigerscientific.com or live chat

Customers are saying

5.00 ★ (3)
Verified