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Biomiga Recombinant Proteinase K, 25 mg/mL, 100 mL (PK2020-02)
Biomiga recombinant Proteinase K PK2020-02, 25 mg/mL in a 100 mL bottle (2.5 g of enzyme), ≥90% purity by SDS-PAGE
Proteinase K retains activity with SDS, urea and guanidine salts and is inactivated by PMSF, per Biomiga
Where Proteinase K fits in DNA and RNA prep: lyse the sample, digest proteins including nucleases, purify the nucleic acid
Biomiga PK2020-02 product facts: 100 mL, 25 mg/mL, recombinant liquid, ≥90% purity, room-temperature stable for at least 60 days, store at −20 °C long term
Biomiga Recombinant Proteinase K, 25 mg/mL, 100 mL (PK2020-02)
Biomiga recombinant Proteinase K PK2020-02, 25 mg/mL in a 100 mL bottle (2.5 g of enzyme), ≥90% purity by SDS-PAGE
Proteinase K retains activity with SDS, urea and guanidine salts and is inactivated by PMSF, per Biomiga
Where Proteinase K fits in DNA and RNA prep: lyse the sample, digest proteins including nucleases, purify the nucleic acid
Biomiga PK2020-02 product facts: 100 mL, 25 mg/mL, recombinant liquid, ≥90% purity, room-temperature stable for at least 60 days, store at −20 °C long term

Biomiga Recombinant Proteinase K, 25 mg/mL, 100 mL (PK2020-02)

$750.00

    Biomiga's recombinant Proteinase K is a serine protease supplied ready to use at 25 mg/mL, in a 100 mL bottle for high-volume DNA and RNA purification. Biomiga states it digests native proteins, inactivating nucleases such as DNase and RNase without a separate denaturation step, and retains activity in the presence of SDS, urea and guanidine salts.

    • 100 mL at 25 mg/mL: 2.5 g of enzyme per bottle
    • ≥90% purity by SDS-PAGE
    • Stable at room temperature for at least 60 days; store at −20 °C long term

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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25 mg/mL
Ready-to-use liquid
100 mL
2.5 g enzyme per bottle
≥90%
Purity by SDS-PAGE
Recombinant
Serine protease

PROTEINASE K · DNA AND RNA PREP

Bulk, ready-to-use Proteinase K for DNA and RNA prep.

Biomiga's recombinant Proteinase K (PK2020-02) is a serine protease supplied as a liquid at 25 mg/mL, in a 100 mL bottle: 2.5 g of enzyme, ready to pipette with no powder to reconstitute. Biomiga states it digests native proteins, inactivating enzymes such as DNase and RNase without a separate denaturation step, and that it retains its activity in the presence of SDS, urea and guanidine salts. Biomiga lists DNA and RNA purification as its application.

For research use only. Not for use in diagnostic procedures.

Where it fits

Where Proteinase K fits in DNA and RNA purification: lyse the sample, digest proteins including nucleases, purify the nucleic acid

Use the Proteinase K amount, temperature and time specified by your kit or lab protocol.

STEP 1

Lyse the sample

Cells or tissue in your lysis buffer, which may contain SDS, urea or guanidine salts.

STEP 2

Digest proteins

Proteinase K breaks down proteins, including DNases and RNases, protecting the nucleic acid.

STEP 3

Purify nucleic acid

Continue with your column, bead or precipitation method.

Works in denaturing lysis buffers

Biomiga Proteinase K retains activity with SDS, urea and guanidine salts and is inactivated by PMSF

Per Biomiga's product specification.

Condition Per Biomiga
SDS Retains activity
Urea Retains activity
Guanidine salts Retains activity
PMSF (phenylmethanesulfonyl fluoride) Inactivates the enzyme

Product specification

Specification Detail
Catalog number PK2020-02
Volume 100 mL
Concentration 25 mg/mL in buffer
Form Liquid; recombinant
Purity ≥90% by SDS-PAGE
Alternate names Protease K, Endopeptidase K, Tritirachium alkaline proteinase
Grade Molecular biology grade
Storage Stable at room temperature for at least 60 days; store at −20 °C long term
Application DNA and RNA purification

Built for

Core and high-throughput labs

Enough enzyme for large extraction runs from a single bottle.

Labs that mix their own buffers

Add Proteinase K to in-house lysis buffers containing SDS, urea or guanidine.

Kit-based workflows

Top up Proteinase K for kits that call for it as a separate reagent.

When this bottle is the right call

  • Volume: 100 mL at 25 mg/mL; no reconstituting powder
  • Denaturing buffers: Biomiga states it stays active with SDS, urea and guanidine salts
  • Room-temperature tolerance: stable for at least 60 days at room temperature, per Biomiga
  • Recombinant: a recombinant enzyme, per Biomiga

Related Biomiga products

Cell & Tissue RNA Mini Kit (R6311)

Total RNA from cells and tissue.

Cell/Tissue RNA Kit Plus (R6321)

Adds a gDNA clearance column.

Tissue and bacterial DNA kits (GD2211, GD2411, MD2528)

Ask us for details and pricing.

Email info@bluetigerscientific.com or text (833) 928-4437 for a quote on any Biomiga product.

Frequently asked questions

Quick facts

What it is: Recombinant Proteinase K, a serine protease, as a ready-to-use liquid

Concentration: 25 mg/mL

Bottle: 100 mL (2.5 g of enzyme)

Purity: ≥90% by SDS-PAGE

Use: In vitro research use only

What concentration and purity is it?

25 mg/mL in buffer, with purity of at least 90% by SDS-PAGE, per Biomiga.

How much enzyme is in the bottle?

100 mL at 25 mg/mL is 2.5 g of Proteinase K.

Does it work in buffers with SDS, urea or guanidine?

Yes. Biomiga states it retains its activity in the presence of SDS, urea and guanidine salts, which are common in DNA and RNA lysis and binding buffers.

How should I store Proteinase K?

Biomiga states it is stable at room temperature for at least 60 days and recommends −20 °C for long-term storage. Check the label for the lot's expiry date.

How do I inactivate it?

Biomiga lists phenylmethanesulfonyl fluoride (PMSF) as an inactivator.

How much should I use per prep?

Follow your extraction kit or lab protocol. Biomiga does not specify a dose for this stock solution.

Is it recombinant?

Yes. Biomiga describes it as recombinant Proteinase K.

Can it be used for clinical or diagnostic work?

No. It is for in vitro research use only.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

    SpecificationDetail
    ManufacturerBiomiga, Inc. (San Diego, CA)
    Catalog NumberPK2020-02
    ProductRecombinant Proteinase K (serine protease)
    Alternate NamesProtease K, Endopeptidase K, Tritirachium alkaline proteinase
    GradeMolecular biology grade
    FormLiquid; dissolved in buffer
    Concentration25 mg/mL
    Volume100 mL (2.5 g of enzyme)
    Purity≥90% by SDS-PAGE
    Activity NotesRetains activity with SDS, urea and guanidine salts; inactivated by PMSF (Biomiga-stated)
    ApplicationDNA and RNA purification
    StorageStable at room temperature for at least 60 days; store at −20 °C long term
    ShippingAmbient temperature, per Biomiga
    Country of OriginUSA
    HazardousConsult the current manufacturer SDS and supplied instructions for buffer hazards, handling, and storage.
    Intended UseIn vitro research use only (RUO)

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

    Payment Terms
    Orders are prepaid by card, or invoiced against an institutional purchase order once credit is approved. Prices are in USD and subject to change without notice. Purchase orders are due within 30 days of invoice unless agreed otherwise in writing.

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    Confirmed orders are placed with the manufacturer and are final. Changes or cancellations must be requested in writing before shipment and approved by Blue Tiger Scientific; approved cancellations may incur restocking, materials or third-party fees.

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    Receiving & Claims
    Inspect every shipment on arrival. Shortages, damage or thawed temperature-sensitive product must be reported to Blue Tiger Scientific in writing, with photos, within 2 business days of delivery. Claims received after that window, and shipments that are refused, unclaimed or left unattended, are not eligible for credit or replacement.

    Returns & Refunds
    Returns require a Return Merchandise Authorization (RMA) issued by Blue Tiger Scientific and must be requested within 30 days of delivery. Refunds or replacements are issued only for shipping errors or verified product defects. Temperature-sensitive products are not returnable once delivered. Customers pay return shipping unless the error was ours, and refunds exclude original shipping charges. Approved refunds are processed within 45 business days.

    Warranty
    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

    Limitation of Liability
    Blue Tiger Scientific's total liability for any claim is limited to the purchase price of the product involved. Blue Tiger Scientific and Biomiga are not liable for indirect, incidental or consequential damages, including lost research time or materials, arising from the use or misuse of any product.

    Safety
    Handle all products according to the Safety Data Sheet and the manufacturer's instructions, using appropriate personal protective equipment.

    Legal & Export Compliance
    Customers must use products in compliance with all applicable local, state, federal and international laws, including export control and biosafety regulations. Products may not be exported or re-exported without the required authorizations.

    Resale
    Products may not be resold or redistributed without prior written approval from Blue Tiger Scientific.

    Force Majeure
    Neither party is liable for delays or failures caused by events beyond its reasonable control, including weather, carrier disruptions, supply shortages or changes in regulation.

    Governing Law
    These terms are governed by the laws of the State of California and the United States.

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