See total iron, not just the readily reacting fraction
Choose digested total iron when suspended material or complexed iron matters to your water result. This matched pre-dosed reagent set supports 0.00–7.00 mg/L as Fe on the documented HI83314 and HI83399 methods.
Factory photograph of the matched reagent format; packaging is illustrative, not a physical contents count.
Select the right method
For surface water, drinking water, groundwater, process control and wastewater workflows requiring total iron. This is not a ferrous-only test or a dissolved-only result: digestion releases iron from organic and inorganic complexes so the phenanthroline reaction can measure the broader fraction.
Matched chemistry simplifies replenishment
The prepared digestion vials and matched additions keep replenishment tied to the total-iron method instead of an unrelated iron reagent. The method uses HI96778V-0 digestion vial, HI96778A-0 reagent A, HI96778B-0 reagent B and PERSULFATE/I potassium persulfate. The set is specified for 25 nominal tests; the US page does not itemize the physical bottle and packet counts, so per-test doses below are not a packing list.
Digest first, then develop the color
Add 8 mL sample to a digestion vial, mix, add one PERSULFATE/I packet and shake vigorously for 60 seconds. Digest at 150 °C for 30 minutes in the specified reactor, cool to room temperature and mix. Add 1 mL reagent A and mix, then zero the same vial. Before adding one reagent B packet, the vial must be 18–22 °C. Shake gently for 30 seconds; allow 3 minutes for color development before reading. Use the complete instrument procedure, not this overview, as the operating instructions.
Keep the total-iron scope intact
The current manuals include ferrous, ferric, dissolved, suspended and complexed iron. Homogenize samples containing suspended solids before digestion; do not routinely remove the suspended fraction to obtain a total-iron result. If turbidity forms after digestion, follow the manual’s filtration direction. Check pH below 1 after sample addition to the digestion vial and 3.8–5.5 after reagent A.
Plan separate equipment
Provide an HI83314 or HI83399 with the method-appropriate vial adapter, HI839800 reactor, cooling rack, accurate 8 mL and 1 mL transfer tools and the required handling setup. The HI839800-01 is a separately sold digestion heater, not an optical reader. Use the manual’s fume-hood and protective-equipment instructions; acidification can release toxic gases from reactive samples. Never use an oven or microwave.
Capacity and method limits
The same sample vial is used for zero and read, so a separate sacrificial blank is not deducted from the 25 nominal tests. QC and repeats consume capacity. The primary range is 0.00–7.00 mg/L as Fe with 0.01 mg/L resolution. Review the full interference list, including chloride above 185000 mg/L and molybdate molybdenum above 50 mg/L. Store unused vials in their packaging in a cool, dark place.
Compatibility and responsible handling
The named vial procedures are documented for HI83314 and HI83399. Use the method and adapter specified for your instrument. Review every matching component SDS and your actual lot instructions. Photometer, accessories and handling equipment are separate.
Frequently Asked Questions
Why choose this instead of a ferrous-iron kit?
This is the digested total-iron method. It includes ferric, complexed and suspended forms as well as ferrous and dissolved iron; a ferrous-only kit answers a different question.
What range and reporting basis does it use?
0.00–7.00 mg/L as total iron, Fe, with 0.01 mg/L resolution on the supported methods.
Is the pack for 25 or 50 tests?
25 nominal tests. The US URL contains an outdated 50-tests slug, but the current product and instrument procedures specify 25. QC and repeats consume reagents.
Is potassium persulfate part of the method?
Yes. The matched chemistry includes PERSULFATE/I alongside the HI96778V-0 vial, reagent A and reagent B. Use one persulfate packet per test as instructed; this is a dose, not an inferred physical packet count in the box.
What heating and cooling are required?
Digest at 150 °C for 30 minutes in the specified reactor, cool to room temperature, then follow the A/zero/B sequence. The vial must be 18–22 °C before adding B. The reactor is separate equipment.
Should suspended iron be filtered out first?
Not as a generic total-iron preparation. Homogenize suspended-solids samples before digestion. If turbidity forms after digestion, follow the manual’s filtration instructions.
Which meters and adapter should I use?
The HI83314 and HI83399 manuals directly document this kit. Select the instrument’s Iron Total vial method and its specified adapter; do not infer compatibility merely from vial fit.
Do I lose a test to a separate blank?
No separate sacrificial vial is specified: zero the same digested sample vial after reagent A, then add B and read. QC and repeat measurements still consume reagents.
