Separate ferrous iron from a total-iron assumption
Measure reactive ferrous iron, Fe²⁺, with the HI96776 reagent specified for Hanna’s Iron(II) program. This phenanthroline method forms an orange-red complex for photometric measurement over 0.00–6.00 mg/L as Fe²⁺ on the verified instruments. It is useful when the oxidation state matters to a water or process-control investigation. The analytical target is ferrous iron—not digested total iron and not a result obtained by intentionally reducing all ferric iron first. Correct temperature and a prompt three-minute reading are essential parts of that distinction.
Official HI96776-0 Iron(II) packet artwork. Printed lot and expiry dates are illustrative and do not describe current supplied stock.
Check the exact catalog number and method
Use the exact Iron(II) method on HI83300, HI83305 or HI83399. HI96776 is not a generic replacement for other Iron LR or total-iron kits. The current method manual governs ferrous-only scope despite broader boilerplate on the US product pages.
A single packet for the ferrous-specific method
The current manufacturer manual describes reactive ferrous iron reacting with 1,10-phenanthroline. Zero the instrument with 10 mL of unreacted sample, add one packet of HI96776-0 and mix gently for the prescribed 30 seconds. Read at the specified three-minute point. The reagent pack simplifies dosing, but it does not remove the need to select Iron(II), maintain clean cuvettes and follow the model’s complete measurement procedure.
Treat temperature and timing as measurement conditions
The method warning requires the sample to be between 18 and 22°C. That is a condition for analysis, not a reagent storage-temperature specification. The manual also warns that reaction times beyond three minutes can allow some ferric iron, Fe³⁺, to react and cause falsely high readings. A late result therefore must not be interpreted as a better measurement of ferrous iron. Organize sample preparation so that the required temperature and timer can be met before starting the reaction.
Match the iron fraction to the question
In oxygenated water, iron chemistry can shift toward ferric forms and precipitation, while low-oxygen water may contain dissolved ferrous iron. The HI96776 method addresses the ferrous fraction defined by its prescribed reaction. It is not a digestion workflow or a substitute for a separately validated total-iron method. Check the exact interference table for the sample matrix; the instrument manual identifies concentration-dependent effects from several ions, so a clear-looking sample is not by itself evidence of an interference-free result.
Protect the reagent and the analyst
The linked US HI96776-0 SDS identifies serious eye irritation and supplies handling, protection and disposal guidance. Keep the original container sealed in a ventilated place away from direct sunlight and incompatible materials, as the SDS directs. Do not eat, drink or smoke during use, and avoid release to the environment. No published shipping weight or air-transport eligibility is asserted here. Consult the actual label and SDS rather than applying the sample’s 18–22°C measurement condition to storage.
Frequently Asked Questions
Does HI96776 measure ferrous iron or total iron?
The exact Iron(II) method measures reactive ferrous iron, Fe²⁺. It is not a digested-total-iron determination and does not intentionally reduce all ferric iron to obtain total iron. Use a separate validated method when total iron is the analytical target.
Why does the supplier description mention ferric reduction?
The US product-page boilerplate includes broader ferric-reduction wording. The current exact instrument manuals explicitly define Iron(II) as the ferrous method and warn that late ferric reaction creates a false high result. This listing follows that method-specific scope, not the conflicting boilerplate.
Which photometers and range are verified?
The HI83300, HI83305 and HI83399 manuals list HI96776-01 and HI96776-03 for their Iron(II) method at 0.00–6.00 mg/L as Fe²⁺. Confirm that exact program rather than selecting a different iron range or kit.
Is 18–22°C the storage temperature?
No. It is the required sample-temperature interval during measurement. The SDS separately directs storage in sealed original containers in a ventilated place away from direct sunlight and incompatible materials. Do not turn a method condition into a storage specification.
Why must I read at three minutes?
Timing is critical. The manual warns that after three minutes some ferric iron can also react, giving falsely high measurements. Follow the timer and do not intentionally extend color development to capture more iron.
How much sample and reagent does one analysis use?
The method uses 10 mL of unreacted sample for zeroing, then one HI96776-0 powder packet, gentle mixing for 30 seconds and the prescribed three-minute reading. Instruments, cuvettes and calibration standards are not included in the reagent offer.
Can these packets replace another Hanna Iron LR kit?
Not solely because both tests mention iron or use a powder packet. Match HI96776 to the Iron(II) method in the instrument manual. Other low-range or total-iron kits can use different chemistry, calibration and preparation steps.
What should I review before handling or reporting a result?
Read the HI96776-0 US SDS and the exact instrument method PDF linked in the Manuals/Brochures tab. Use eye protection and other controls specified by the SDS, check the matrix interference table and record the Fe²⁺ reporting basis. Factory image dates are illustrative; inspect supplied packaging for its actual lot and expiry.
