Thymosin β4 Human – Research Use Only

Thymosin β4 Human is a 5.2 kDa actin-sequestering peptide supplied as a 100 µg lyophilized powder (Catalog #B2013845). This biotechnology-grade recombinant human Thymosin β4 (also known as Tβ4, Seraspenide, or Hematopoietic system regulatory peptide) plays essential roles in actin dynamics, cell migration, wound healing, angiogenesis, and tissue repair. It is widely used in research on regeneration, inflammation, cardiovascular biology, and cytoskeletal regulation.

Scientific Overview

Thymosin β4 is a highly conserved 43-amino acid peptide that sequesters monomeric G-actin, regulating actin polymerization and depolymerization. Beyond cytoskeletal control, it promotes cell migration, angiogenesis, wound healing, and anti-inflammatory responses. It is involved in tissue regeneration, cardioprotection, and modulation of the hematopoietic system. Recombinant human Thymosin β4 is a valuable tool for studying these processes in cell biology, regenerative medicine, and inflammation research.

This Thymosin β4 Human is suitable for:

• Actin dynamics and cytoskeletal regulation studies
• Cell migration, wound healing, and angiogenesis assays
• Research on tissue repair and regenerative medicine
• Investigation of anti-inflammatory and cardioprotective mechanisms
• Functional studies of the hematopoietic system

Usage & Handling Guidance

Store the lyophilized powder at −20 °C. Reconstitute in sterile ultrapure water or PBS to the desired concentration. Prepare single-use aliquots to avoid repeated freeze–thaw cycles. Reconstituted solutions should be kept on ice during use or stored frozen.

• Recommended applications: Cell migration, wound healing, and actin-binding studies
• Working concentration: Typically 0.1–10 µg/mL (optimize for specific assay)
• Stability: Lyophilized form is stable at −20 °C; reconstituted aliquots should be used promptly or frozen
• Handling: Use sterile techniques; protect from repeated freeze–thaw cycles

What You Get

• 100 µg recombinant human Thymosin β4 as lyophilized powder
• 5.2 kDa actin-sequestering peptide with high purity
• Biotechnology-grade material suitable for sensitive biological assays
• Convenient quantity for multiple experiments
• For research use only (RUO)

Why Researchers Choose It

• Authentic recombinant human Thymosin β4 for accurate functional studies
• Key regulator of actin dynamics, cell migration, and wound healing
• Important tool for research in regeneration, angiogenesis, and inflammation
• High-purity lyophilized format ensures stability and easy reconstitution
• Well-documented roles in cytoskeletal control and tissue repair

Frequently Asked Questions (FAQ)

• What is the main function of Thymosin β4?
  It sequesters G-actin, regulates actin polymerization, and promotes cell migration, wound healing, and angiogenesis.
• How should I reconstitute the powder?
  Reconstitute in sterile ultrapure water or PBS. Prepare aliquots and store at −20 °C to avoid repeated freeze–thaw cycles.
• What concentration is typically used?
  Working concentrations are usually 0.1–10 µg/mL depending on the assay (optimize empirically).
• Is this suitable for in vivo studies?
  This product is for research use only (RUO) and not intended for therapeutic or clinical applications.
• Does it have other names?
  Yes, it is also known as Tβ4, Seraspenide, and Hematopoietic system regulatory peptide.

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References

• Xing Y, Ye Y, Zuo H, Li Y. Progress on the Function and Application of Thymosin β4. Front Endocrinol (Lausanne). 2021 Dec 21;12:767785.
• Gladka MM, Kohela A, Molenaar B, Versteeg D, Kooijman L, Monshouwer-Kloots J, Kremer V, Vos HR, Huibers MMH, Haigh JJ, Huylebroeck D, Boon RA, Giacca M, van Rooij E. Cardiomyocytes stimulate angiogenesis after ischemic injury in a ZEB2-dependent manner. Nat Commun. 2021 Jan 4;12(1):84.
• Hannappel E. beta-Thymosins. Ann N Y Acad Sci. 2007 Sep;1112:21-37.
• Yu R, Mao Y, Li K, Zhai Y, Zhang Y, Liu S, Gao Y, Chen Z, Liu Y, Fang T, Zhao M, Li R, Xu J, Chen W. Recombinant Human Thymosin Beta-4 Protects against Mouse Coronavirus Infection. Mediators Inflamm. 2021 Apr 21;2021:9979032.
• Jo JO, Kang YJ, Ock MS, Kleinman HK, Chang HK, Cha HJ. Thymosin β4 expression in human tissues and in tumors using tissue microarrays. Appl Immunohistochem Mol Morphol. 2011 Mar;19(2):160-7.
• Hoch K, Volk DE. Structures of Thymosin Proteins. Vitam Horm. 2016;102:1-24.
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• Elitsur Y, Mutchnick MG, Sakr WA, Luk GD. Thymosin alpha 1 and thymosin beta 4 modulate human colonic lamina propria lymphocyte function. Immunopharmacology. 1990 Sep-Oct;20(2):89-96.
• Vasilopoulou E, Riley PR, Long DA. Thymosin-β4: A key modifier of renal disease. Expert Opin Biol Ther. 2018 Jul;18(sup1):185-192.
• Wang M, Feng LR, Li ZL, Ma KG, Chang KW, Chen XL, Yang PB, Ji SF, Ma YB, Han H, Ruganzu JB, Yang WN, Qian YH. Thymosin β4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-κB signaling axis in APP/PS1 mice. J Neuroinflammation. 2021 Jun 28;18(1):146.