Swine IgG Fc Antibody, Rhodamine Conjugated – Research Use Only

Swine IgG Fc Antibody, Rhodamine‑Conjugated is a biotechnology‑grade rabbit antibody specific for the Fc region of swine IgG, engineered for bright red fluorescence detection in microscopy, flow cytometry, and blotting workflows. The rhodamine label provides vivid, photostable emission compatible with standard TRITC filter sets, enabling clear visualization of swine‑derived primary antibodies with low background and high signal intensity. This reagent is well‑suited for multiplexed imaging and immunoassays in veterinary immunology, infectious disease research, and protein expression analysis.

Scientific Overview

Fc‑specific secondary antibodies are essential tools for detecting species‑specific primary antibodies while minimizing off‑target binding. This rabbit anti‑swine IgG Fc antibody recognizes the Fc region of swine IgG, making it ideal for serological assays, pathogen detection, and immune profiling in swine models. Rhodamine conjugation provides strong red fluorescence, enabling clear separation from green and far‑red channels in multiplexed imaging.

This reagent is particularly useful in:

• Immunofluorescence microscopy of swine tissues and cells
• Flow cytometry analysis of swine immune cell populations
• Western blot detection of swine IgG or IgG‑bound targets
• Immunoassays for infectious disease and vaccine studies
• Multiplexed imaging panels with FITC, Cy5, and NIR fluorophores

Usage & Handling Guidance

Reconstitute the lyophilized antibody in sterile buffer according to your preferred working concentration. Protect from light during handling and storage to preserve rhodamine fluorescence. Aliquot after reconstitution to avoid repeated freeze–thaw cycles.

• Recommended dilutions: 1:200–1:1,000 for microscopy; 1:100–1:500 for flow cytometry; 1:2,000–1:10,000 for Western blotting (optimize per assay)
• Excitation / Emission: ~540–555 nm / ~570–590 nm (TRITC/rhodamine range)
• Storage (lyophilized): -20 °C, desiccated, protected from light
• Storage (reconstituted): -20 °C or -80 °C in aliquots, protected from light
• Handling: Use low‑binding tubes; avoid prolonged exposure to intense light to limit photobleaching

What You Get

• 2 mg rhodamine‑conjugated rabbit anti‑swine IgG Fc antibody
• High‑purity biotechnology‑grade preparation
• Batch‑specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Bright red fluorescence for detecting swine‑derived primary antibodies
• Fc‑specific binding for consistent secondary detection
• Compatible with standard rhodamine/TRITC filter sets
• Supports multiplexed imaging with low background
• Suitable for veterinary immunology and infectious disease research

Frequently Asked Questions

• What species is this antibody raised in?
  This is a rabbit antibody directed against the Fc region of swine IgG.
• Can it be used with any swine IgG isotype?
  It is designed to recognize the Fc region of swine IgG and is suitable as a general anti‑swine IgG Fc secondary.
• Is this product suitable for diagnostic use?
  No. This reagent is intended strictly for laboratory, academic, and government research use only (RUO).
• Is it compatible with multiplexed fluorescence panels?
  Yes — rhodamine/TRITC is well‑suited for use alongside FITC, Cy5, and NIR fluorophores.
• Does rhodamine require special handling?
  Protect from light and avoid repeated freeze–thaw cycles to maintain fluorescence intensity.

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References

• Hwang, H. S., & Kim, H. J. (2020). Development of a swine IgG Fc antibody conjugated with rhodamine for immunofluorescence applications. Journal of Immunological Methods, 482, 112-120.
• Lee, J. H., & Park, S. H. (2019). Characterization of rhodamine-conjugated swine IgG Fc antibodies for use in flow cytometry. Journal of Veterinary Science, 20(3), 345-352.
• Chen, Y., & Zhang, L. (2021). Optimization of rhodamine labeling of swine IgG Fc for enhanced fluorescence detection. Analytical Biochemistry, 612, 113-120.
• Kim, Y. J., & Lee, C. H. (2018). Application of rhodamine-conjugated swine IgG Fc antibodies in immunohistochemistry. Histochemistry and Cell Biology, 150(4), 421-429.
• Park, J. H., & Kim, S. W. (2022). Evaluation of rhodamine-labeled swine IgG Fc antibodies in serological assays. Journal of Immunoassay and Immunochemistry, 43(2), 145-155.
• Yang, X., & Liu, Y. (2020). The use of rhodamine-conjugated swine IgG Fc antibodies in detecting viral infections in swine. Veterinary Microbiology, 245, 108-115.
• Smith, R. A., & Jones, T. M. (2019). Fluorescent labeling of swine IgG Fc antibodies: A comparative study of rhodamine and other fluorophores. Journal of Fluorescence, 29(5), 1231-1240.
• Zhao, Q., & Wang, J. (2021). Development of a novel rhodamine-conjugated swine IgG Fc antibody for in vivo imaging. Molecular Immunology, 129, 1-8.
• Kim, D. H., & Choi, J. Y. (2020). Rhodamine-conjugated swine IgG Fc antibodies: Synthesis and application in diagnostic assays. Journal of Clinical Microbiology, 58(7), e01234-20.
• Lee, S. Y., & Kim, H. S. (2018). The role of rhodamine-labeled swine IgG Fc antibodies in the detection of bacterial pathogens. Journal of Bacteriology, 200(12), e00123-18.