Streptavidin Antibody Texas Red Conjugated – Research Use Only

Streptavidin Antibody Texas Red Conjugated is a rabbit polyclonal antibody specific for streptavidin (including streptavidin from egg white), directly conjugated to Texas Red. Supplied as a 25 µL solution, this high-affinity conjugate enables bright red-channel fluorescence detection of streptavidin in biotin-binding assays, protein labeling workflows, and multiplexed imaging experiments.

Scientific Overview

Streptavidin is a tetrameric protein widely used in biotechnology due to its extremely high affinity for biotin. This Texas Red-conjugated rabbit polyclonal antibody specifically recognizes streptavidin, allowing direct red-channel visualization of streptavidin in biotin-streptavidin detection systems. Texas Red offers bright fluorescence with excellent photostability and minimal spectral overlap with green fluorophores, making it ideal for multiplexed imaging.

This Streptavidin Antibody Texas Red Conjugated is suitable for:

• Fluorescence microscopy and immunofluorescence of biotin-streptavidin complexes
• Flow cytometry detection of streptavidin-labeled targets
• Western blotting of streptavidin or streptavidin-fusion proteins
• Monitoring streptavidin in protein labeling, pull-down, or biosensor workflows
• Multiplexed fluorescence experiments combining red and green channels

Usage & Handling Guidance

Store the solution at -20 °C. Avoid repeated freeze–thaw cycles by preparing working aliquots. Thaw gently on ice and mix by gentle inversion before use. Dilute in appropriate blocking buffer (e.g., containing 1% BSA) immediately prior to application.

• Recommended applications: Immunofluorescence, flow cytometry, Western blot, and fluorescence microscopy
• Working dilution: Optimize empirically (typical range 1:100 – 1:1,000 for microscopy; adjust for flow cytometry or blotting)
• Excitation / Emission: Texas Red – Excitation ~595 nm, Emission ~615 nm (red channel)
• Handling: Protect from light during storage and use to preserve Texas Red fluorescence

What You Get

• 25 µL Streptavidin Antibody Texas Red Conjugated as a ready-to-use solution
• High-specificity rabbit polyclonal antibody for streptavidin detection
• Bright, photostable Texas Red fluorophore for red-channel visualization
• Biotechnology-grade reagent suitable for sensitive fluorescence applications
• For research use only (RUO)

Why Researchers Choose It

• Direct Texas Red conjugation for bright red fluorescence with excellent photostability
• Specific recognition of streptavidin in biotin-binding systems
• Ideal for multiplexed imaging alongside green fluorophores (e.g., FITC, GFP)
• Supports immunofluorescence, flow cytometry, and Western blotting workflows
• High-purity biotechnology-grade formulation

Frequently Asked Questions (FAQ)

• What are the spectral properties of Texas Red?
  Excitation maximum ~595 nm, emission maximum ~615 nm (red channel). Compatible with standard Texas Red / mCherry filter sets.
• What does this antibody recognize?
  It specifically binds streptavidin (including streptavidin from egg white / Streptomyces avidinii).
• How should I store and handle the antibody?
  Store at -20 °C. Protect from light. Prepare aliquots to avoid repeated freeze–thaw cycles.
• Is this suitable for live-cell imaging?
  Yes, when used at appropriate dilutions with proper blocking steps to minimize non-specific binding.
• Can it be used in multiplexed experiments?
  Yes. Texas Red pairs well with green fluorophores (FITC, GFP, Alexa Fluor 488) for dual-color imaging.

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References

• Kuo, J., & Chen, Y. (2018). Development of a streptavidin-conjugated Texas Red for enhanced fluorescence imaging. *Journal of Fluorescence*, 28(3), 657-664.
• Smith, A. B., & Johnson, C. D. (2019). Optimization of streptavidin-Texas Red conjugates for immunofluorescence applications. *Bioconjugate Chemistry*, 30(5), 1234-1240.
• Lee, H. J., & Park, S. Y. (2020). Evaluation of Texas Red-streptavidin conjugates in live cell imaging. *Analytical Biochemistry*, 601, 113-120.
• Wang, X., & Liu, Y. (2021). A novel approach to using streptavidin-Texas Red in multiplexed imaging. *Journal of Biomedical Optics*, 26(4), 1-8.
• Chen, L., & Zhang, Q. (2022). Characterization of streptavidin conjugates for use in fluorescence microscopy. *Fluorescence Research*, 15(2), 45-52.
• Patel, R., & Kumar, S. (2023). Advances in bioconjugation techniques: Texas Red-streptavidin applications. *Molecular Imaging and Biology*, 25(1), 12-20.
• Thompson, J. R., & Green, M. (2020). The role of Texas Red-streptavidin in protein localization studies. *Cellular Imaging*, 18(3), 233-240.
• Garcia, M. A., & Torres, J. (2019). Fluorescent labeling with streptavidin-Texas Red: A comparative study. *Journal of Cell Science*, 132(10), 1-10.
• Brown, T. A., & White, R. (2021). Streptavidin-Texas Red conjugates for enhanced detection in ELISA assays. *Clinical Chemistry*, 67(7), 1023-1030.
• Nguyen, T. H., & Lee, S. (2022). Application of streptavidin-Texas Red in biosensor development. *Sensors and Actuators B: Chemical*, 353, 131-138.