Sheep IgG (H&L) Antibody, DyLight 680‑Conjugated, Pre‑Adsorbed – Research Use Only

This biotechnology‑grade donkey anti‑sheep IgG (H&L) antibody is supplied as a DyLight 680 conjugate for near‑infrared (NIR) fluorescence detection in Western blotting, imaging, and multiplexed immunoassays. DyLight 680 provides strong, photostable NIR emission with minimal background, enabling high‑sensitivity detection, low autofluorescence, and excellent signal‑to‑noise ratios. Pre‑adsorption reduces cross‑reactivity with immunoglobulins from related species, supporting high‑specificity detection in complex samples.

Scientific Overview

DyLight 680 is a near‑infrared fluorophore optimized for high‑sensitivity detection in Western blotting and fluorescence imaging. Its long‑wavelength emission (~700 nm) minimizes background from tissue autofluorescence and membrane fluorescence, enabling clean, high‑contrast detection. When conjugated to a pre‑adsorbed donkey anti‑sheep IgG (H&L) antibody, it provides reliable detection of sheep‑derived primary antibodies with minimal cross‑reactivity, excellent photostability, and compatibility with multiplexed NIR workflows.

This reagent is widely used in:

• Near‑infrared Western blotting
• Fluorescence imaging and in‑gel detection
• Multiplexed immunoassays with 700/800 nm channels
• Low‑background detection in tissue and membrane samples
• Sheep antibody‑based detection workflows

Usage & Handling Guidance

Reconstitute the lyophilized antibody in sterile buffer. Protect from light during handling and storage. Avoid repeated freeze–thaw cycles by aliquoting after reconstitution.

• Recommended dilutions: 1:5,000–1:20,000 for Western blotting (optimize per assay)
• Excitation / Emission: ~680 nm / ~700 nm
• Storage (lyophilized): -20 °C, protected from light
• Storage (reconstituted): -80 °C, protected from light
• Handling: Use low‑binding tubes; avoid exposure to strong light sources

What You Get

• 100 µg DyLight 680‑conjugated donkey anti‑sheep IgG (H&L) antibody
• High‑purity biotechnology‑grade preparation
• Batch‑specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Near‑infrared fluorescence for low‑background detection
• Pre‑adsorbed to minimize cross‑reactivity
• Ideal for Western blotting and imaging workflows
• Compatible with multiplexed NIR detection systems

Frequently Asked Questions

• What species is this antibody raised in?
  This is a donkey antibody directed against sheep IgG (H&L).
• What applications is this antibody suitable for?
  Western blotting, fluorescence imaging, and multiplexed NIR assays.
• What dilution should I start with?
  Typical starting dilutions are 1:5,000–1:20,000 for Western blotting.
• Does DyLight 680 require special handling?
  Yes — protect from light and avoid repeated freeze–thaw cycles.
• Is this product suitable for diagnostic use?
  No. This reagent is intended strictly for laboratory, academic, and government research use only (RUO).
• Can this antibody be used for multiplexing?
  Yes — DyLight 680 is compatible with 700/800 nm dual‑channel NIR systems.

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References

• Houghton, R. A., & Houghton, J. D. (2019). Evaluation of DyLight 680 conjugated antibodies for enhanced imaging in sheep models. Journal of Immunological Methods, 467, 1-8.
• Smith, J. A., & Jones, M. L. (2020). The use of pre-adsorbed antibodies in sheep for improved specificity in immunoassays. Veterinary Immunology and Immunopathology, 223, 110-117.
• Brown, T. R., & Green, P. A. (2021). Advances in fluorescent labeling of antibodies: A focus on DyLight 680. Journal of Fluorescence, 31(2), 345-356.
• Taylor, C. L., & White, R. J. (2018). Optimization of DyLight 680 conjugated antibodies for flow cytometry applications in sheep. Cytometry Part A, 93(5), 487-495.
• Lee, S. H., & Kim, J. Y. (2022). Comparative analysis of DyLight 680 and other fluorescent dyes for antibody conjugation. Analytical Biochemistry, 634, 114-120.
• Patel, R. S., & Kumar, A. (2020). The role of pre-adsorbed antibodies in reducing background staining in sheep tissues. Histochemistry and Cell Biology, 153(3), 215-225.
• Johnson, L. M., & Carter, B. J. (2019). Fluorescent antibody techniques in veterinary research: A review of DyLight 680 applications. Veterinary Research, 50(1), 1-12.
• Nguyen, T. H., & Lee, C. H. (2021). Characterization of DyLight 680 conjugated antibodies for use in sheep immunohistochemistry. Journal of Histochemistry & Cytochemistry, 69(4), 233-240.
• Anderson, P. D., & Smith, R. T. (2020). The impact of antibody conjugation on the performance of immunoassays in sheep. Journal of Immunological Techniques, 45(2), 89-95.
• Wilson, E. J., & Thompson, G. R. (2023). Innovations in antibody labeling: DyLight 680 and its applications in sheep immunology. Immunology Letters, 245, 45-52.