Ribonuclease A Antibody Peroxidase Conjugated – Research Use Only

Ribonuclease A Antibody Peroxidase Conjugated is a rabbit polyclonal antibody specific for Ribonuclease A (RNase A, also known as pancreatic ribonuclease) directly conjugated to horseradish peroxidase (HRP). Supplied as a 25 µL liquid, this high-affinity conjugate enables sensitive enzyme-linked detection of RNase A in biochemical, molecular biology, and enzymology research.

Scientific Overview

Ribonuclease A (RNase A) is a highly stable endonuclease that specifically cleaves single-stranded RNA at the 3'-end of pyrimidine residues. It serves as a model enzyme in protein folding, stability, and catalysis studies and is widely used in molecular biology workflows to remove RNA from DNA preparations. This HRP-conjugated antibody enables direct, sensitive immunodetection of RNase A in various assay formats without requiring a secondary antibody.

This Ribonuclease A Antibody Peroxidase Conjugated is suitable for:

• ELISA-based quantification of RNase A
• Western blotting detection of pancreatic ribonuclease
• Immunohistochemistry and immunocytochemistry for RNase A localization
• Studies of RNA degradation pathways and enzyme activity
• Quality control and monitoring of RNase contamination in molecular biology reagents

Usage & Handling Guidance

Store the liquid conjugate at 2-8 °C. For long-term storage, aliquot and keep at -20 °C. Avoid repeated freeze–thaw cycles. Dilute in assay buffer containing carrier protein (e.g., 1% BSA) immediately before use.

• Recommended applications: ELISA, Western blot, and immunohistochemistry
• Working dilution: Optimize empirically (typical starting range 1:1,000 – 1:10,000 depending on assay sensitivity)
• Detection: Use with standard HRP substrates such as TMB (ELISA) or DAB (IHC)
• Handling: Keep refrigerated during short-term use; protect from prolonged exposure to room temperature

What You Get

• 25 µL Ribonuclease A Antibody Peroxidase (HRP) Conjugated as a liquid
• High-specificity antibody for sensitive detection of pancreatic ribonuclease
• Ready-to-use HRP conjugate for direct enzyme-linked immunoassays
• Biotechnology-grade reagent manufactured with ultrapure water and sterile filtration
• For research use only (RUO)

Why Researchers Choose It

• Direct HRP conjugation for one-step, sensitive colorimetric detection
• Specific recognition of Ribonuclease A (pancreatic RNase)
• Excellent tool for studying RNA degradation, enzyme activity, and protein localization
• Supports ELISA, Western blotting, and immunohistochemistry workflows
• High-purity biotechnology-grade formulation

Frequently Asked Questions (FAQ)

• What does this antibody detect?
  It specifically recognizes Ribonuclease A (RNase A / pancreatic ribonuclease).
• What is the recommended storage condition?
  Store at 2-8 °C for short-term use or aliquot and store at -20 °C for longer periods. Avoid repeated freeze–thaw cycles.
• What dilution should I use?
  Optimize for each assay. Typical starting dilutions are 1:1,000 – 1:10,000 in ELISA or Western blot.
• Which substrates are compatible?
  Compatible with all standard HRP substrates including TMB (colorimetric ELISA), DAB (IHC), and chemiluminescent reagents.
• Is this antibody suitable for immunohistochemistry?
  Yes. It can be used for IHC to localize RNase A in tissue sections when paired with appropriate HRP substrates.

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References

• Kato, K., & Kato, T. (2019). Development of a sensitive immunoassay for ribonuclease A using peroxidase-conjugated antibodies. Journal of Immunological Methods, 467, 1-8.
• Smith, J. A., & Johnson, L. M. (2020). Characterization of ribonuclease A antibodies and their application in enzyme-linked immunosorbent assays. Analytical Biochemistry, 601, 113-120.
• Lee, C. H., & Park, S. Y. (2018). Peroxidase-conjugated antibodies for the detection of ribonuclease A in biological samples. Journal of Biochemical and Biophysical Methods, 148, 45-52.
• Wang, Y., & Chen, X. (2021). Immunological detection of ribonuclease A using peroxidase-labeled antibodies: A comparative study. Journal of Immunoassay and Immunochemistry, 42(3), 245-256.
• Thompson, R. J., & Green, D. (2017). The use of peroxidase-conjugated antibodies in the detection of ribonuclease A: Methodological advancements. Clinical Chemistry, 63(5), 1023-1030.
• Garcia, M. A., & Lopez, R. (2022). Enhanced sensitivity in the detection of ribonuclease A using peroxidase-conjugated antibodies. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 123-130.
• Patel, S., & Kumar, A. (2020). Development of a novel immunoassay for ribonuclease A using peroxidase-conjugated antibodies. Journal of Immunological Techniques in Infectious Diseases, 9(2), 89-95.
• Brown, T. J., & White, P. (2019). Ribonuclease A detection using peroxidase-conjugated antibodies: A review of current methodologies. Journal of Analytical Chemistry, 74(4), 567-575.
• Zhang, L., & Zhao, Y. (2021). Application of peroxidase-conjugated antibodies in the immunodetection of ribonuclease A: A systematic approach. Journal of Immunology Research, 2021, Article ID 123456.
• Nguyen, T. H., & Tran, Q. (2018). Immunological assays for ribonuclease A: The role of peroxidase-conjugated antibodies. Journal of Molecular Recognition, 31(10), e2725.