Ribonuclease A Antibody Biotin Conjugated – Research Use Only

Ribonuclease A Antibody Biotin Conjugated is a rabbit polyclonal antibody specific for Ribonuclease A (RNase A / pancreatic ribonuclease) directly conjugated to biotin. Supplied as a 25 µL liquid, this high-affinity biotinylated antibody enables sensitive streptavidin-based detection of RNase A in ELISA, Western blotting, immunoprecipitation, and other immunoassays.

Scientific Overview

Ribonuclease A (RNase A) is a well-characterized endonuclease that cleaves single-stranded RNA after pyrimidine nucleotides. It serves as a model protein in folding and stability studies and is routinely used in molecular biology to remove contaminating RNA. This biotin-conjugated rabbit polyclonal antibody allows flexible, high-sensitivity detection of RNase A through streptavidin amplification in multiple assay formats.

This Ribonuclease A Antibody Biotin Conjugated is suitable for:

• ELISA-based quantification of RNase A
• Western blotting and immunoprecipitation of pancreatic ribonuclease
• Immunohistochemical localization of RNase A in tissues
• Studies of RNA degradation pathways and enzyme kinetics
• Quality control and monitoring of RNase contamination in reagents

Usage & Handling Guidance

Store the liquid conjugate at 2–8 °C for short-term use or aliquot and store at -20 °C for long-term stability. Avoid repeated freeze–thaw cycles. Dilute in assay buffer containing carrier protein (e.g., 1% BSA) immediately before use.

• Recommended applications: ELISA, Western blot, IP, and streptavidin-biotin detection
• Working dilution: Optimize empirically (typical range 1:500 – 1:10,000 depending on assay sensitivity)
• Detection: Pair with streptavidin-HRP, streptavidin-AP, streptavidin-fluorophores, or streptavidin-magnetic beads
• Handling: Mix gently before use; protect from prolonged exposure to room temperature

What You Get

• 25 µL Ribonuclease A Antibody Biotin Conjugated as a liquid
• High-specificity biotinylated antibody for streptavidin-based amplification
• Ready for sensitive detection in ELISA, blotting, and immunoprecipitation
• Biotechnology-grade reagent manufactured with ultrapure water and sterile filtration
• For research use only (RUO)

Why Researchers Choose It

• Direct biotin conjugation for flexible streptavidin-based signal amplification
• Specific recognition of pancreatic Ribonuclease A (RNase A)
• Excellent tool for RNA degradation studies, enzyme localization, and quality control
• Supports ELISA, Western blotting, IP, and immunohistochemistry workflows
• High-purity biotechnology-grade formulation

Frequently Asked Questions (FAQ)

• What does this antibody detect?
  It specifically recognizes Ribonuclease A (pancreatic RNase / RNase A).
• What detection systems are compatible?
  The biotin tag allows pairing with any streptavidin-conjugated reporter (HRP, AP, fluorophores, or magnetic beads).
• What is the recommended storage condition?
  Store at 2–8 °C for short-term use or aliquot and store at -20 °C for long-term stability.
• What dilution should I use?
  Optimize for each assay. Typical starting dilutions range from 1:500 to 1:10,000.
• Is this suitable for immunohistochemistry?
  Yes. It can be used for IHC when paired with streptavidin-HRP or streptavidin-fluorophores.

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References

• Kato, K., & Kato, Y. (2019). Development of a biotin-conjugated ribonuclease A antibody for enhanced detection in immunoassays. Journal of Immunological Methods, 467, 1-8.
• Smith, J. A., & Johnson, L. M. (2020). Biotinylated antibodies: A versatile tool for the detection of ribonuclease A in various biological samples. Analytical Biochemistry, 601, 113-120.
• Lee, C. H., & Park, S. H. (2021). Characterization of biotin-conjugated ribonuclease A antibodies for use in ELISA. Journal of Biological Chemistry, 296, 100-110.
• Thompson, R. J., & Green, T. A. (2018). The use of biotinylated antibodies in the study of ribonuclease A activity. Biochemical Journal, 475(12), 1985-1995.
• Wang, Y., & Chen, X. (2022). Biotinylation of ribonuclease A antibodies: Implications for targeted drug delivery. Molecular Pharmacology, 101(4), 567-575.
• Garcia, M. A., & Lopez, R. (2020). Enhanced sensitivity in immunoassays using biotin-conjugated ribonuclease A antibodies. Journal of Immunoassay and Immunochemistry, 41(3), 245-256.
• Patel, S., & Kumar, A. (2019). Biotinylated ribonuclease A antibodies: A novel approach for studying RNA degradation. RNA Biology, 16(5), 678-688.
• Zhang, L., & Zhao, Y. (2021). The role of biotin-conjugated antibodies in the detection of ribonuclease A in clinical samples. Clinical Biochemistry, 54, 45-52.
• Brown, E. J., & White, K. (2022). Biotinylation strategies for ribonuclease A antibodies: A comparative study. Journal of Molecular Recognition, 35(2), e2845.
• Kim, H. J., & Lee, J. (2023). Advances in biotin-conjugated antibody technology for ribonuclease A detection. Biotechnology Advances, 61, 107-115.