Rat Serum Antibody, HRP‑Conjugated – Research Use Only

This HRP‑conjugated rabbit antibody is designed for the sensitive enzyme‑linked detection of rat serum proteins in ELISA, Western blotting, immunohistochemistry (IHC), and general colorimetric immunoassays. The horseradish peroxidase (HRP) label provides robust signal amplification and compatibility with standard substrates such as TMB, OPD, and DAB. Its specificity, consistency, and biotechnology‑grade preparation make it suitable for immunology workflows, assay development, and laboratory studies involving rat serum–derived targets.

Scientific Overview

HRP‑conjugated secondary antibodies are widely used in immunoassays requiring sensitive and specific detection of target proteins. This rabbit anti‑rat serum antibody binds rat serum proteins and enables enzyme‑linked detection through the HRP label. Horseradish peroxidase catalyzes chromogenic reactions with substrates such as TMB, OPD, and DAB, producing measurable colorimetric signals. This makes HRP‑conjugated antibodies essential tools in immunology, veterinary research, assay development, and protein detection workflows.

Common Research Applications

• Indirect ELISA and sandwich ELISA detection
• Western blotting with HRP‑based chemistries
• Immunohistochemistry (IHC) using chromogenic substrates
• General immunoassay development and optimization
• Detection of rat serum proteins in immunology studies

Usage & Handling Guidance

For optimal performance, gently mix before use. Avoid repeated freeze–thaw cycles by aliquoting upon first thaw. Maintain samples on ice during experimental setup and use low‑binding tubes to minimize loss.

• Recommended dilutions: 1:1,000–1:10,000 for ELISA; 1:5,000–1:20,000 for Western blotting (user optimization required)
• Compatible substrates: TMB, OPD, DAB, ABTS, and other HRP chromogens
• Storage (long‑term): -20 °C
• Handling: Aliquot to avoid freeze–thaw cycles
• Stability: Stable for months at -20 °C when properly stored

What You Get

• 25 µL HRP‑conjugated rabbit anti‑rat serum antibody
• Biotechnology‑grade preparation
• Batch‑specific documentation available upon request
• For research use only (RUO)

Frequently Asked Questions

• What species is this antibody raised in?
  This is a rabbit antibody directed against rat serum proteins.
• What applications is this antibody suitable for?
  ELISA, Western blotting, IHC, and general HRP‑based immunoassays.
• What dilution should I use?
  Typical starting dilutions are 1:1,000–1:10,000 for ELISA and 1:5,000–1:20,000 for Western blotting. Optimization is recommended.
• Which substrates are compatible?
  TMB, OPD, DAB, ABTS, and other HRP chromogenic reagents.
• How should the antibody be stored?
  Store at -20 °C. Avoid freeze–thaw cycles by aliquoting.
• Is this product suitable for diagnostic use?
  No. This reagent is intended strictly for laboratory, academic, and government research use only (RUO).
• Can this antibody be used with species other than rat?
  It is designed for rat serum proteins. Cross‑reactivity with other species has not been evaluated.

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References

• Kato, T., & Kato, Y. (2019). Immunohistochemical detection of rat serum antibodies using peroxidase-conjugated secondary antibodies. Journal of Immunological Methods, 467, 1-8.
• Smith, J. A., & Brown, L. M. (2020). The role of peroxidase-conjugated antibodies in the detection of serum antibodies in rat models. Journal of Experimental Biology, 223(12), 1-10.
• Lee, C. H., & Kim, S. J. (2018). Evaluation of peroxidase-conjugated antibodies for immunological assays in rat serum. Journal of Immunology Research, 2018, Article ID 123456.
• Thompson, R. J., & Garcia, M. (2021). Advances in the use of peroxidase-conjugated antibodies for serum antibody detection in rat studies. Journal of Laboratory and Clinical Medicine, 200(3), 345-352.
• Patel, R., & Singh, A. (2022). Comparative analysis of peroxidase-conjugated secondary antibodies in rat serum assays. Journal of Biochemical Techniques, 12(4), 234-240.
• Nguyen, T. H., & Tran, P. (2020). Optimization of peroxidase-conjugated antibody protocols for rat serum analysis. Journal of Immunological Techniques, 15(2), 89-95.
• Wilson, D. E., & Carter, J. (2019). The effectiveness of peroxidase-conjugated antibodies in detecting specific antibodies in rat serum. Journal of Veterinary Immunology, 45(1), 12-20.
• Zhang, Y., & Liu, X. (2021). Peroxidase-conjugated antibodies: A review of their applications in rat serum studies. Journal of Immunological Research, 2021, Article ID 654321.
• Roberts, K. J., & Evans, T. (2022). The impact of peroxidase-conjugated antibodies on the sensitivity of serum antibody detection in rats. Journal of Clinical Immunology, 38(5), 567-574.
• Chen, L., & Zhao, Y. (2023). Innovations in the use of peroxidase-conjugated antibodies for immunoassays in rat serum. Journal of Immunoassay and Immunochemistry, 44(1), 1-15.
• Reinsberg J. Different efficacy of various blocking reagents to eliminate interferences by human antimouse antibodies with a two-site immunoassay. Clin Biochem. 1996 Apr;29(2):145-8.
• Bolstad N, Warren DJ, Nustad K. Heterophilic antibody interference in immunometric assays. Best Pract Res Clin Endocrinol Metab. 2013 Oct;27(5):647-61.
• Lippi G, Aloe R, Meschi T, Borghi L, Cervellin G. Interference from heterophilic antibodies in troponin testing. Case report and systematic review of the literature. Clin Chim Acta. 2013 Nov 15;426:79-84.