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Phosphorylase Enzyme (Highly Pure) Proteins Molecular Depot
Phosphorylase Enzyme (Highly Pure) Proteins Molecular Depot
Phosphorylase Enzyme (Highly Pure) Proteins Molecular Depot
Phosphorylase Enzyme (Highly Pure) Proteins Molecular Depot

Phosphorylase Enzyme (Highly Pure)

$907.00

    Catalog Number: B2013048 (0.1 mg)

    Phosphorylase Enzyme (Highly Pure) is a high quality enzyme that catalyses the conversion of glycogen to glucose-1-phosphate and glucose. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Phosphorylase Enzyme (Highly Pure) – Catalog Number: B2013048 (0.1 mg)

Catalog number: B2013048
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 0.1 mg
Molecular Weight or Concentration: N/A
Supplied as: Lyophilized Powder
Applications: molecular tool for various biochemical applications
Storage: −20 °C
Keywords: glucose-6-phosphate
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity > 18 MΩ-cm) and are filtered through 0.22 um.

Scientific Description

The Phosphorylase Enzyme (Highly Pure) (Catalog B2013048, 0.1 mg, lyophilized powder) catalyzes the phosphorolytic conversion of glycogen into glucose-1-phosphate and free glucose. Delivered as a high purity lyophilized reagent, this enzyme provides exceptional stability and dosage control, making it well-suited for sensitive biochemical workflows and enzymology applications requiring traceability.

Its activity in cleaving glycogen is extensively utilized in metabolic assays, pathway elucidation, and biosensor development linked to carbohydrate metabolism. Researchers value this enzyme for constructing reproducible assay platforms, modeling glycogenolytic flux, and calibrating phosphate or glucose detection systems. The lyophilized format facilitates convenience in storage, aliquoting, and multi-lab coordination.

Common applications include quantitative glucose-1-phosphate generation studies, optimization of glycogen degradation assays, and validation of enzymatic cascades involving phosphorylases. Combined with the product’s high-grade purification and safe cold-chain preservation, this enzyme upgrades standard operating protocols and integrates seamlessly with laboratory information management systems (LIMS).

Why researchers choose this product:

  • Lyophilized powder ensures stable preservation and accurate reconstitution
  • Efficient catalytic conversion of glycogen to glucose-1-phosphate and glucose supports metabolic and detection assays
  • Biotechnology-grade purity ensures consistency across high-sensitivity workflows
  • Reliable performance in SOP-driven environments and multi-user lab systems

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • Johnson LN, Barford D, Owen DJ, Noble ME, Garman EF. From phosphorylase to phosphorylase kinase Adv Second Messenger Phosphoprotein Res. 1997;31:11-28.
  • Krause EG, Rabitzsch G, Noll F, Mair J, Puschendorf B. Glycogen phosphorylase isoenzyme BB in diagnosis of myocardial ischaemic injury and infarction Mol Cell Biochem. 1996 Jul-Aug;160-161:289-95.
  • Johnson LN, Hu SH, Barford D. Catalytic mechanism of glycogen phosphorylase Faraday Discuss. 1992;(93):131-42.
  • Gäde G. Glycogen phosphorylase in the fat body of two cockroach species, _Periplaneta americana_ and _Nauphoeta cinerea_: isolation, partial characterization of three forms and activation by hypertrehalosaemic hormones Z Naturforsch C J Biosci. 1991 Jan-Feb;46(1-2):149-62.
  • Vul’fson PL. [Glycogen phosphorylase of skeletal muscles] _Biokhimiia_. 1986 Dec;51(12):1974-92.
  • Leonidas DD, Zographos SE, Tsitsanou KE, Skamnaki VT, Stravodimos G, Kyriakis E. Glycogen phosphorylase revisited: extending the resolution of the R- and T-state structures of the free enzyme and in complex with allosteric activators _Acta Crystallogr F Struct Biol Commun_. 2021 Sep 1;77(Pt 9):303-311.
  • Livanova NB, Chebotareva NA, Eronina TB, Kurganov BI. Pyridoxal 5′-phosphate as a catalytic and conformational cofactor of muscle glycogen phosphorylase B _Biochemistry (Mosc)_. 2002 Oct;67(10):1089-98.
  • Eronina TB, Mikhaylova VV, Chebotareva NA, Kleymenov SY, Pivovarova AV, Kurganov BI. Combined action of chemical chaperones on stability, aggregation and oligomeric state of muscle glycogen phosphorylase b _Int J Biol Macromol_. 2022 Apr 1;203:406-416.
  • Fischer EH. Phosphorylase and the origin of reversible protein phosphorylation _Biol Chem_. 2010 Feb-Mar;391(2-3):131-137.
  • Migocka-Patrzałek M, Lewicka A, Elias M, Daczewska M. The effect of muscle glycogen phosphorylase (Pygm) knockdown on zebrafish morphology _Int J Biochem Cell Biol_. 2020 Jan;118:105658.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

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    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

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    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
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    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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