MST2 Protein, Active – Research Use Only

MST2 Protein, Active is a biotechnology-grade active serine/threonine kinase reagent supplied as 10 µg recombinant protein for research applications involving Hippo pathway signaling, kinase assays, phosphorylation studies, and biochemical pathway analysis. MST2 (also known as STK3) is a core upstream kinase in the Hippo signaling pathway and plays a major role in regulating cell growth, apoptosis, tissue homeostasis, regeneration, and tumor suppression. This active kinase is especially useful in workflows related to cancer biology, cell signaling, pathway modulation, kinase inhibitor screening, and mechanistic molecular biology research.

Scientific Overview

MST2 (STK3) is a central serine/threonine kinase in the Hippo signaling pathway, where it contributes to the regulation of cell proliferation, apoptosis, organ size control, stress response, and tissue regeneration. Because MST2 functions upstream of key pathway effectors such as LATS kinases and YAP/TAZ-associated signaling networks, it is widely studied in experimental systems involving signal transduction, tumor suppression, regenerative biology, and kinase-dependent pathway regulation.

In laboratory workflows, active MST2 protein is commonly used for in vitro kinase assays, phosphorylation studies, substrate evaluation, signaling cascade reconstruction, inhibitor screening, and mechanistic pathway analysis. It is particularly relevant to researchers investigating Hippo pathway biology, cancer signaling, tissue repair pathways, stem cell regulation, and kinase-targeted therapeutic strategies.

Applications

• Hippo pathway signaling and pathway reconstruction studies
• In vitro kinase assays and phosphorylation workflows
• Kinase inhibitor screening and target validation
• Cancer biology and tumor suppression research
• Regeneration, stem cell, and mechanistic signaling studies

Usage & Handling Guidance

Store at -20°C as specified. Researchers should thaw and handle under conditions appropriate for active kinase preservation and biochemical assay consistency. Working concentration, reaction conditions, cofactors, and substrate compatibility should be optimized according to the intended kinase assay or signaling workflow.

• Preparation: Prepare according to the intended kinase assay or signaling protocol
• Handling: Minimize unnecessary freeze-thaw exposure to help preserve activity
• Optimization: Adjust concentration and reaction conditions based on substrate and assay format
• Workflow note: Validate activity and assay compatibility in the intended biochemical system

What You Get

• 10 µg of active MST2 protein
• Biotechnology-grade kinase reagent for signaling research
• Suitable for Hippo pathway and phosphorylation workflows
• Useful in kinase assays and mechanistic biochemical studies
• For research use only (RUO)

Why Researchers Choose It

• Directly relevant to Hippo pathway and kinase signaling studies
• Useful for phosphorylation assays and inhibitor screening workflows
• Supports mechanistic cancer and regeneration research
• Applicable to target validation and pathway analysis experiments
• High-value active protein format for controlled biochemical use

Frequently Asked Questions (FAQ)

• What is MST2 Protein used for in research?
  It is commonly used in Hippo pathway signaling studies, kinase assays, phosphorylation workflows, inhibitor screening, and mechanistic biochemical research.
• What is MST2?
  MST2 (also known as STK3) is a serine/threonine kinase that functions as a core upstream regulator in the Hippo signaling pathway.
• Why is MST2 important in signaling biology?
  MST2 plays an important role in regulating cell growth, apoptosis, tissue homeostasis, regeneration, and tumor suppression through Hippo pathway signaling networks.
• Can this protein be used in kinase inhibitor screening workflows?
  Yes. Active MST2 protein is commonly relevant to inhibitor testing, substrate evaluation, and pathway-targeting assay development.
• Is this product intended for diagnostic or therapeutic use?
  No. This product is supplied strictly for research use only and is not intended for diagnostic or therapeutic applications.

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References

• Hong AW, Meng Z, Guan KL. The Hippo pathway in intestinal regeneration and disease Nat Rev Gastroenterol Hepatol. 2016 Jun;13(6):324-37.
• Galan JA, Avruch J. MST1/MST2 Protein Kinases: Regulation and Physiologic Roles Biochemistry. 2016 Oct 4;55(39):5507-5519.
• Qi S, Zhu Y, Liu X, Li P, Wang Y, Zeng Y, Yu A, Wang Y, Sha Z, Zhong Z, Zhu R, Yuan H, Ye D, Huang S, Ling C, Xu Y, Zhou D, Zhang L, Yu FX. WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy Mol Cell. 2022 May 19;82(10):1850-1864.e7.
• Xu C, Wang L, Zhang Y, Li W, Li J, Wang Y, Meng C, Qin J, Zheng ZH, Lan HY, Mak KK, Huang Y, Xia Y. Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms J Am Soc Nephrol. 2020 May;31(5):946-961.
• Snchez-Sanz G, Matallanas D, Nguyen LK, Kholodenko BN, Rosta E, Kolch W, Buchete NV. MST2-RASSF protein-protein interactions through SARAH domains Brief Bioinform. 2016 Jul;17(4):593-602.
• Wang P, Geng J, Gao J, Zhao H, Li J, Shi Y, Yang B, Xiao C, Linghu Y, Sun X, Chen X, Hong L, Qin F, Li X, Yu JS, You H, Yuan Z, Zhou D, Johnson RL, Chen L. Macrophage achieves self-protection against oxidative stress-induced ageing through the Mst-Nrf2 axis Nat Commun. 2019 Feb 14;10(1):755.
• Fan F, He Z, Kong LL, Chen Q, Yuan Q, Zhang S, Ye J, Liu H, Sun X, Geng J, Yuan L, Hong L, Xiao C, Zhang W, Sun X, Li Y, Wang P, Huang L, Wu X, Ji Z, Wu Q, Xia NS, Gray NS, Chen L, Yun CH, Deng X, Zhou D. Pharmacological targeting of kinases MST1 and MST2 augments tissue repair and regeneration Sci Transl Med. 2016 Aug 17;8(352):352ra108.
• Sun X, Ren Z, Cun Y, Zhao C, Huang X, Zhou J, Hu R, Su X, Ji L, Li P, Mak KLK, Gao F, Yang Y, Xu H, Ding J, Cao N, Li S, Zhang W, Lan P, Sun H, Wang J, Yuan P. Hippo-YAP signaling controls lineage differentiation of mouse embryonic stem cells through modulating the formation of super-enhancers Nucleic Acids Res. 2020 Jul 27;48(13):7182-7196.
• Kudryashova TV, Dabral S, Nayakanti S, Ray A, Goncharov DA, Avolio T, Shen Y, Rode A, Pena A, Jiang L, Lin D, Baust J, Bachman TN, Graumann J, Ruppert C, Guenther A, Schmoranzer M, Grobs Y, Eve Lemay S, Tremblay E, Breuils-Bonnet S, Boucherat O, Mora AL, DeLisser H, Zhao J, Zhao Y, Bonnet S, Seeger W, Pullamsetti SS, Goncharova EA. Noncanonical HIPPO/MST Signaling via BUB3 and FOXO Drives Pulmonary Vascular Cell Growth and Survival Circ Res. 2022 Mar 4;130(5):760-778.