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Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno
Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope Microscope Meiji Techno

Meiji Techno TC-5400L LED Trinocular Inverted Phase-Contrast Microscope

$7,530.00

    Observe transparent living specimens with a clear setup plan. The Meiji TC-5400L is an LED trinocular inverted microscope with supplied 10× and 20× phase optics for routine culture observation.

    • 100× / 200× visual magnification; two 10× eyepieces included.
    • Phase slider, centering telescope and plain stage supplied.
    • Mechanical stage, vessel holders and camera system are optional—choose exact parts below.

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MEIJI TECHNO · INVERTED PHASE CONTRAST

See living cells.
Keep your culture in its vessel.

The TC-5400L combines transmitted LED illumination, 10× and 20× phase objectives and a trinocular head for routine observation of transparent specimens. Start with the supplied optical system, then choose positioning and imaging accessories around your workflow.

100× and 200× visual magnification included. Mechanical stage, vessel holders, camera and camera adapter are separate.

TC-5400L manufacturer photograph with standard plain stage.
TC-5400L manufacturer photograph with standard plain stage. · Select image to enlarge.

100× / 200× supplied

Two MA817 10× eyepieces plus MA826 10× and MA827 20× phase objectives. The five-position nosepiece is not a five-objective package.

73 mm condenser clearance

Working distance of the supplied MA853 condenser. Objective working distances are different: 7.6 mm at 10× and 7.0 mm at 20×.

Trinocular + front camera port

Two distinct imaging connection paths. Neither includes a camera or adapter; select one matched path rather than buying both by default.

A usable phase microscope—not an accessory guessing game

Included optical system

TC-5400L stand and MA816/10 trinocular head; two MA817 10×/FN22 eyepieces; MA826 10× and MA827 20× phase objectives; MA853 condenser; MA855 phase slider; MA458/05 centering telescope; MA861 green phase filter; plain stage with MA878 glass insert.

Optional workflow upgrades

MA380/05 mechanical stage and a matching vessel holder; MA825 4× phase objective; camera and appropriate adapter. The standard plain stage is already supplied. No fluorescence module, heater, incubator or environmental control is included.

Choose the microscope above for the standard configuration. Use the component grids for individual extras; photographs and application illustrations do not change what is supplied.

Why inverted geometry matters for culture work

TC-5400L side view: illumination above the specimen and objectives below the stage.
TC-5400L side view: illumination above the specimen and objectives below the stage. · Select image to enlarge.

Observe through the vessel bottom. The objectives sit below the stage while transmitted illumination comes from above. That arrangement lets you examine suitable cells and specimens in dishes or culture vessels rather than transferring every sample onto a conventional slide.

The long-working-distance optics help accommodate culture work, but vessel fit is not just a diameter question. Bottom thickness and optical quality, vessel height, stage support and access to the region of interest all matter.

Do not use 73 mm as a universal vessel-height limit. It is a condenser specification—not a guarantee that every flask or multi-well plate fits or images well.

Choose your TC-5400L setup by the work you do

Choose by what happens at your bench—not by magnification alone.

Application illustration: Routine culture checks, not an image captured through the TC-5400L.

APPLICATION ILLUSTRATION

Routine culture checks

Observe morphology and growth changes. Inspect transparent living cells in suitable culture vessels without routine fixation or staining. The supplied 100×/200× visual settings are the starting point.

Plan around the vessel: verify its footprint, bottom optics and access before selecting a holder. This is not an incubator or a validated viability/sterility assay.

Application illustration: Controlled specimen positioning, not an image captured through the TC-5400L.

APPLICATION ILLUSTRATION

Controlled specimen positioning

Choose the stage around the sample. Add MA380/05 when repeatable X–Y positioning is useful, then choose a holder for the actual dish, slide, counting chamber or Terasaki plate.

The holder does not replace the mechanical stage. A nominal dish size alone does not establish optical compatibility.

Application illustration: Capture and compare observations, not an image captured through the TC-5400L.

APPLICATION ILLUSTRATION

Capture and compare observations

Plan the camera connection first. For a compatible C-mount camera on the front port, select MA877. A top-phototube installation uses a different adapter route and needs a camera-specific check.

Camera, software and adapter are not supplied. Image scale and field coverage depend on the selected camera and optical path.

Original generated application illustrations, not manufacturer photographs, actual microscope images or promises of vessel/camera compatibility. Authentic instrument and accessory photographs are identified separately.

Phase contrast or brightfield? Use the mode that reveals the specimen

Phase contrast · Transparent specimens

Select the matching phase annulus to turn optical phase differences into visible brightness differences. Useful for unstained living cells and thin transparent specimens. Check annulus centering with the supplied telescope; thick specimens, thick vessel bottoms and phase halos can limit the result.

Brightfield · Absorbing or colored detail

Move the phase slider to its empty brightfield position. This is useful when the specimen already creates visible contrast. Transparent unstained cells may remain difficult to distinguish; closing the aperture excessively trades away brightness and resolution.

The supplied MA855 is one slider: a 4× annulus, a shared 10×/20×/40× annulus and a brightfield position. The 4× position is ready for an optional MA825 objective; it does not mean a 4× objective is already included.

Start with one of three buying paths

1 · Standard observation

Choose TC-5400L alone when the supplied plain stage suits your vessel and positioning needs. Eyepieces, phase objectives, condenser, slider and centering telescope are already part of this configuration.

2 · Add controlled positioning

Choose TC-5400L + MA380/05 + a holder matched to your vessel. For example, the 35 mm dish route uses MA384. Do not order every holder as a required set.

3 · Add documentation

Choose the microscope and the imaging path your camera requires. MA877 is the front-port C-mount attachment; a suitable camera is separate. A top-port adapter is not interchangeable by assumption.

These are component shopping lists, not new bundled SKUs or a claim that optional parts are included in the microscope price.

Positioning and vessel holders · Select the stage, then the holder

MA380/05 adds 112 × 72 mm X–Y travel with drop-down coaxial controls. A suitable holder must be selected separately. The MA878 glass insert supplied with the plain stage does not fit MA380/05.

Need a different vessel? Ask us to check it before ordering. Meiji also lists other holder formats; the options here are a focused selection, not a universal vessel-compatibility chart.

Three fit checks worth making before checkout

Mechanical fit

Tell us the vessel manufacturer/model, outside footprint and height. A 56 × 87 mm Terasaki holder is not a general-purpose holder for full-size SBS plates.

Optical fit

Confirm bottom thickness and optical quality against the objective and specimen. A physically supported dish can still produce poor phase contrast if its bottom is unsuitable.

Workflow fit

Decide whether hand positioning is sufficient, controlled X–Y motion is needed, and whether the work requires imaging or environmental control beyond the supplied microscope.

Choose the field you need—not just a bigger number

Objective Visual magnification with supplied 10× eyepieces Numerical aperture Objective working distance
MA826 · included 10× phase / 100× total 0.25 7.6 mm
MA827 · included 20× phase / 200× total 0.40 7.0 mm
MA825 · optional 4× phase / 40× total 0.13 17.3 mm

The optional MA825 provides a lower-power view for finding a region before switching to detail. Use the slider’s 4× annulus with that objective. The supplied MA853 condenser has NA 0.30 and 73 mm working distance; those are not the objective values in this table.

A 40× phase objective is another possible expansion, but it reduces objective working distance to 2.8 mm. Meiji recommends the MA854 NA 0.55 condenser for improved 40× phase observation; its working distance is 20.5 mm. Request a complete vessel/optics check rather than treating this as a no-compromise upgrade.

Low-power optics and front-port camera connection

Two camera paths. One matched imaging plan.

Front port: MA877 connects a compatible C-mount camera to the TC-5000 front port. It has no internal lens; the documented 0.66× factor belongs to the front-port optical system. It is not a 0.66× lens for the top phototube.

Trinocular phototube: the MA816/10 head offers a selectable split of 100% to the eyetubes, or 80% to the photo path and 20% to the eyetubes. This route requires its own matched adapter and camera.

Before selecting a camera, confirm mount, sensor size, required field coverage, computer/connection requirements and whether calibrated measurements are needed. Neither port makes a camera, software or measurement calibration part of this order.

MA877 manufacturer photograph: optional front-port C-mount attachment, camera not included.
MA877 manufacturer photograph: optional front-port C-mount attachment, camera not included. · Select image to enlarge.

Set up the phase system deliberately

1 · Start with low power

Secure the vessel and begin with a supplied low-power objective. Watch objective-to-stage clearance when changing objectives; long working distance does not prevent collisions.

2 · Match and center the annulus

Use the shared annulus for the supplied 10×/20× phase objectives. Check the phase ring alignment with the supplied centering telescope and follow the manual’s alignment procedure.

3 · Adjust for the specimen

Focus on the specimen plane, adjust illumination and use the supplied green phase filter as appropriate. If contrast is poor, check annulus, centering, bottom optics and specimen thickness before assuming more magnification will help.

Choose a different instrument when the job changes

TC-5400L is a transmitted brightfield/phase-contrast culture-observation microscope. It is not a supplied fluorescence, heated live-cell imaging or micromanipulation workstation. Do not choose it on the assumption that GFP imaging, CO₂ control, time-lapse automation or clinical validation is included.

For low-power dissection, specimen sorting or electronics surface inspection, compare the Meiji EMZ-5 stereo microscope setup guide. It serves a different task and is not an accessory for TC-5400L.

TC-5400L buying questions

Is this TC-5400L binocular or trinocular?

This is the trinocular TC-5400L configuration with the manufacturer-listed MA816/10 head. It has two viewing eyepieces plus a top phototube. Meiji’s dealer model matrix distinguishes it from the binocular TC-5300L, even though one manufacturer webpage title incorrectly says binocular.

What magnifications are included?

100× and 200× visual magnification: two 10× eyepieces with the supplied 10× and 20× phase objectives. The nosepiece has five positions, but only those two objectives are included. MA825 4× is optional and gives 40× total with the same eyepieces.

Are the phase slider and centering telescope supplied?

Yes. The standard phase configuration lists MA855 slider, MA458/05 centering telescope and MA861 green phase filter. There is one slider with a 4× position, a shared 10×/20×/40× position and a brightfield opening—not three separately supplied sliders.

Do I need to add a mechanical stage?

Not for every application: the microscope includes a plain stage with glass insert. If controlled X–Y movement is needed, add MA380/05 and a suitable holder. The included MA878 insert fits the plain stage, not the optional MA380/05.

Will my dish, flask or multi-well plate fit?

We need the exact vessel model and dimensions to check. Footprint, height, bottom thickness/optical quality, stage support and access all matter. The condenser’s 73 mm working distance is not a universal maximum vessel height, and a named dish holder does not guarantee optical performance through every dish bottom.

Does the trinocular head mean a camera is included?

No. Camera and adapter are separate. MA877 is the front-port C-mount attachment, with no internal lens; the documented 0.66× is the TC front-port system factor. A top-phototube setup needs its own camera/adapter check.

Can it perform fluorescence or incubated live-cell imaging as supplied?

No fluorescence module, incubator, heater or CO₂ control is included in this configuration. Phase contrast can help observe suitable living cells without fixation or staining, but it is not a guarantee of environmental control, viability measurement or sterility testing.

Is the manual specific to the LED version?

The official TC5000 manual covers series optics, phase alignment and mechanical operation, but contains legacy halogen lamp instructions. This listing is for the LED TC-5400L; do not use legacy halogen relamping steps for LED service. Ask us to coordinate current LED service guidance.

Can I request one quote for the microscope and selected accessories?

Yes. Add the microscope and chosen components to the Quote Cart and check exact quantities before submitting. Each is a separate line; a stage may also need a selected holder, and a camera adapter does not include a camera. Availability, shipping and the requested configuration are confirmed with the quote.

    SpecificationTC-5400L
    Manufacturer / modelMeiji Techno TC-5400L
    Instrument typeInverted biological microscope; transmitted brightfield and phase contrast
    Optical systemInfinity corrected; F = 200 mm
    IlluminationTransmitted LED with intensity adjustment; not a supplied fluorescence system
    Viewing headMA816/10 Siedentopf trinocular; 30° inclination; 53–75 mm interpupillary adjustment
    Head beam splitterSelectable 100% to eyetubes, or 80% photo / 20% eyetubes
    Eyepieces suppliedTwo MA817 SWH10X high-eyepoint eyepieces; FN22
    Objectives suppliedMA826 10× phase, NA 0.25, WD 7.6 mm; MA827 20× phase, NA 0.40, WD 7.0 mm
    Visual magnification supplied100× and 200× with supplied 10× eyepieces
    NosepieceQuintuple; two objectives supplied
    CondenserMA853 ELWD; NA 0.30; working distance 73 mm
    Phase sliderOne MA855: 4× annulus, shared 10×/20×/40× annulus, brightfield opening
    Phase alignment / filterMA458/05 centering telescope and MA861 546 nm green interference filter
    Standard stagePlain stage, 180 × 245 mm; MA878 glass insert, 109 mm diameter and 44.3 mm opening
    Optional positioningMA380/05 mechanical stage; 112 × 72 mm travel; suitable holder selected separately
    Camera connectionFront camera port and trinocular phototube; camera and adapters separate
    Electrical configurationAutomatic voltage-sensing supply; localized power cord. Confirm destination requirements before ordering.
    Not includedMechanical stage, optional holders/objectives, camera/adapter/software, fluorescence module, heater or incubator

    Ordering information

    Catalog numberOrder scope
    TC-5400LStandard LED trinocular phase-contrast microscope with the supplied optics and plain stage listed above
    MA380/05Mechanical stage — optional; Drop-down coaxial controls
    MA378Slide / hemocytometer holder — optional; Requires optional MA380/05
    MA38435 mm dish holder — optional; Requires optional MA380/05
    MA38555 mm dish holder — optional; Requires optional MA380/05
    MA864Terasaki plate holder — optional; Requires optional MA380/05
    MA8254× phase objective — optional; NA 0.13 · 17.3 mm working distance
    MA877Front-port C-mount adapter — optional; No internal lens · camera separate

    Blue Tiger Scientific is an authorized reseller of Meiji Techno products. We manage your purchase and order-return requests. Meiji Techno handles its manufacturer warranty directly.

    Ordering & delivery

    Availability and dispatch timing depend on the selected microscope and accessories. Delivery dates shown on this page are estimates, not guarantees; supplier lead time, processing and carrier transit can affect arrival. Contact Blue Tiger before ordering if you have a firm deadline or specific destination requirements.

    Inspect your delivery

    Check your shipment as soon as it arrives. Keep all original cartons and packing materials. Report damage, shortages or an incorrect item to Blue Tiger immediately, with photographs of the item, packaging and shipping label, so we can meet Meiji’s five-day damage/shortage reporting requirement.

    Order returns — start with Blue Tiger

    All order-return requests must be initiated through Blue Tiger Scientific at info@bluetigerscientific.com. For eligible, non-defective products, request authorization within 14 days of delivery. A request does not guarantee acceptance; eligibility and written authorization must be confirmed before shipment.

    • Include your Blue Tiger order number, manufacturer part number, serial number if applicable, quantity and reason for return.
    • Wait for written authorization and the designated return address. Do not send an order return to our business address or directly to Meiji without these instructions.
    • Return approved items complete, unused and in new, resalable condition, with all original packaging, components, manuals and accessories. Follow the return instructions and use prepaid, tracked and fully insured shipping.

    Approved non-defective returns are subject to a 15% restocking fee. Original outbound shipping is non-refundable, including shipping costs incurred on orders advertised with free shipping. Return shipping and insurance are the customer’s responsibility. Damage, incorrect-item and defect reports are reviewed separately from unwanted-product returns.

    Custom-made or special-order items and products identified as non-returnable are excluded from standard non-defective returns. Used, incomplete or non-resalable items are not eligible. Contact us before purchase if return eligibility is important to your selection.

    Manufacturer warranty — contact Meiji directly

    Meiji Techno provides and administers the manufacturer warranty. Contact Meiji directly for warranty coverage questions, registration, claims and repair authorization: 1-800-832-0060, Product Service Department.

    Blue Tiger Scientific does not process warranty claims, authorize warranty repairs or provide warranty service. Have your model, serial number and proof of purchase available when contacting Meiji. Do not send an item for warranty service until Meiji provides authorization and shipping instructions.

    Coverage is governed by Meiji’s applicable written warranty, including exclusions, registration requirements and any separate electrical-component coverage. A limited lifetime instrument warranty does not mean every component is covered for life.

    International orders

    International customers should request a quote to confirm the configuration, destination and shipping arrangements. Import duties, taxes and brokerage charges are the customer’s responsibility unless expressly included in the quote. International returns are not permitted under Blue Tiger’s published return policy. Confirm warranty availability and service arrangements for your country directly with Meiji.

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