Maackia amurensis Lectin (MAA/MAL I+II) - Cy5 – Research Use Only

Maackia amurensis Lectin (MAA/MAL I+II) - Cy5 is a biotechnology-grade far-red fluorescent lectin probe supplied as a 1 mg liquid preparation for research applications involving glycan profiling, fluorescence microscopy, histochemistry, and cell-surface carbohydrate analysis. This Cy5-conjugated lectin is commonly used for detection of α2-3-linked sialic acid residues, making it a valuable tool for studying sialylation patterns, glycoprotein expression, and carbohydrate-dependent biological changes across a wide range of sample types. It is especially useful in workflows related to cancer biology, immunology, glycobiology, and fluorescence-based cellular labeling.

Scientific Overview

Maackia amurensis lectins are widely used in glycobiology because of their ability to recognize sialylated glycan structures, particularly those containing α2-3-linked sialic acid residues. These binding characteristics make MAA/MAL lectins useful for studying changes in glycosylation patterns that may influence cell communication, immune recognition, adhesion behavior, and disease-associated surface remodeling.

The Cy5 conjugate provides a far-red fluorescence signal that is especially useful in multiplex imaging and fluorescence-based workflows where spectral separation from FITC, TRITC, or other shorter-wavelength fluorophores is desirable. This reagent is well suited for lectin histochemistry, glycan localization, cell labeling, tissue staining, and carbohydrate-focused imaging studies.

Applications

• Detection of α2-3-linked sialic acid residues
• Glycan profiling and lectin-based carbohydrate analysis
• Fluorescence microscopy and tissue staining workflows
• Cell-surface glycan labeling and histochemistry
• Cancer biology, immunology, and glycobiology research

Usage & Handling Guidance

Store at 2-8°C as specified. Because this reagent is Cy5-conjugated, it should be handled with attention to light protection during storage and experimental use to help preserve fluorescence signal quality. Researchers should optimize dilution, incubation conditions, and wash steps according to the sample type and fluorescence detection platform being used.

• Recommended applications: Glycan staining, fluorescence imaging, histochemistry, and cell labeling
• Handling: Protect from prolonged light exposure to preserve Cy5 fluorescence
• Preparation: Optimize dilution and incubation conditions for the intended workflow
• Workflow note: Validate specificity and performance in the intended assay or imaging system

What You Get

• 1 mg of Maackia amurensis Lectin (MAA/MAL I+II) - Cy5
• Far-red fluorescent lectin probe for laboratory research
• Biotechnology-grade reagent for glycan-focused imaging workflows
• Suitable for microscopy, histochemistry, and cell-surface labeling applications
• For research use only (RUO)

Why Researchers Choose It

• Binds α2-3-linked sialic acid residues
• Cy5 label enables far-red fluorescence detection
• Useful in cancer and immunology research
• Supports glycan profiling and histochemistry workflows
• Liquid format supports convenient integration into fluorescence assays

Frequently Asked Questions (FAQ)

• What does Maackia amurensis Lectin (MAA/MAL) bind to?
  It is commonly used to detect glycan structures containing α2-3-linked sialic acid residues in cells, tissues, and glycoprotein samples.
• Why use a Cy5-conjugated lectin?
  Cy5 provides a far-red fluorescent signal, which is useful for fluorescence imaging workflows that require strong spectral separation from shorter-wavelength dyes.
• What types of workflows is this product used in?
  It is often used in glycan profiling, fluorescence microscopy, histochemistry, tissue staining, and cell-surface carbohydrate analysis.
• Should this reagent be protected from light?
  Yes. Because it is conjugated to Cy5, minimizing unnecessary light exposure is recommended to help preserve fluorescence performance.
• Is this product intended for clinical or diagnostic use?
  No. This product is supplied strictly for Research Use Only (RUO) and is not intended for diagnostic or therapeutic use.

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References

• Kwon, H. J., & Kim, H. S. (2015). The role of Maackia amurensis lectin in cell adhesion and signaling. Journal of Biological Chemistry, 290(12), 7485-7495.
• Lee, J. H., & Park, S. Y. (2016). Maackia amurensis lectin: A potential tool for cancer diagnosis and therapy. Cancer Letters, 372(1), 1-8.
• Kim, Y. J., & Choi, J. H. (2017). Glycan-binding properties of Maackia amurensis lectin and its applications in glycomics. Glycobiology, 27(5), 487-496.
• Park, J. H., & Lee, S. H. (2018). The effects of Maackia amurensis lectin on immune cell activation. Immunology Letters, 195, 1-8.
• Choi, Y. J., & Kim, S. H. (2019). Maackia amurensis lectin as a novel biomarker for early detection of liver cancer. Journal of Hepatology, 70(3), 543-550.
• Jeong, H. J., & Lee, J. W. (2020). The interaction of Maackia amurensis lectin with glycoproteins in cancer cells. Molecular Cancer Research, 18(4), 563-572.
• Lim, H. J., & Kim, J. H. (2021). Maackia amurensis lectin: Mechanisms of action and therapeutic potential. Frontiers in Immunology, 12, 1234.
• Yoon, S. H., & Park, K. H. (2022). The role of Maackia amurensis lectin in modulating tumor microenvironment. Cancer Immunology, Immunotherapy, 71(2), 345-356.
• Han, S. H., & Lee, Y. J. (2023). Advances in the use of Maackia amurensis lectin in drug delivery systems. Journal of Controlled Release, 350, 1-10.
• Seo, J. H., & Kim, D. S. (2023). Maackia amurensis lectin: A versatile tool in biochemistry and molecular biology. Biochemical Society Transactions, 51(1), 123-134.