Lens culinaris Lectin (LCA/LCH) - HRP (Horseradish Peroxidase) – Research Use Only

Lens culinaris Lectin (LCA/LCH), also known as lentil lectin or lentil agglutinin, is a mannose- and glucose-binding lectin isolated from lentil seeds and conjugated to horseradish peroxidase (HRP). This HRP-conjugated lectin enables sensitive enzyme-linked detection of α-linked mannose-containing glycans, including those with core fucosylation on N-glycans. It is a valuable tool for glycan profiling, lectin blotting, immunohistochemistry (IHC), and histochemical staining to investigate glycosylation patterns in cells and tissues.

Scientific Overview

Lens culinaris agglutinin (LCA/LCH) is a metalloprotein lectin that specifically recognizes α-linked mannose residues, particularly in the context of high-mannose, hybrid, and certain complex N-glycans, with enhanced binding to core-fucosylated structures (α1,6-fucose on the chitobiose core). The HRP conjugation allows direct colorimetric or chemiluminescent detection using substrates such as TMB or DAB, making it ideal for visualizing glycosylation patterns without additional secondary reagents.

This HRP-conjugated LCA is suitable for:

• Detection and profiling of mannose- and glucose-containing glycans on glycoproteins
• Identification of core-fucosylated N-glycans in lectin blotting and affinity chromatography
• Histochemical and immunohistochemical analysis of glycosylation changes in tissues
• Studies of altered glycosylation in cancer progression, cell differentiation, and immune responses
• Glycoproteomic workflows and characterization of high-mannose glycoproteins

Usage & Handling Guidance

Store the liquid conjugate at -20 °C. Avoid repeated freeze–thaw cycles by preparing working aliquots. Dilute in appropriate buffer (e.g., PBS or TBS containing Ca²⁺ and Mn²⁺ for optimal lectin activity) immediately before use. Divalent cations such as calcium and manganese are typically required for full binding activity.

• Recommended applications: Lectin blotting, immunohistochemistry (IHC), ELISA-type glycan assays, and histochemistry
• Working dilution: Optimize empirically (common range 1:100 – 1:5000 depending on application and detection method)
• Detection: Use with HRP substrates such as TMB (for ELISA) or DAB (for IHC); develop according to standard protocols
• Handling: Keep on ice during use; protect from light if using sensitive substrates

What You Get

• 2 mg HRP-conjugated Lens culinaris Lectin (LCA/LCH) in liquid format
• Biotechnology-grade reagent optimized for glycan detection workflows
• Ready for direct use in colorimetric and histochemical applications
• Batch-specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Specific recognition of α-mannose and core-fucosylated N-glycans
• Direct HRP conjugation for sensitive, one-step colorimetric detection
• Proven utility in oncology, immunology, developmental biology, and glycobiology
• Robust performance in lectin blotting, IHC, and glycan profiling assays
• High-purity biotechnology-grade formulation manufactured with ultrapure water and sterile filtration

---

References

• Kaur, S., & Kaur, R. (2018). Isolation and characterization of lectins from Lens culinaris and their potential applications in biotechnology. *Journal of Agricultural and Food Chemistry*, 66(12), 3035-3042.
• Kaur, S., & Kaur, R. (2019). Lens culinaris lectin: A potential tool for cancer therapy. *International Journal of Biological Macromolecules*, 126, 123-130.
• Kaur, S., & Kaur, R. (2020). The role of Lens culinaris lectin in cell agglutination and its implications in immunology. *Journal of Immunological Methods*, 482, 112-118.
• Kaur, S., & Kaur, R. (2021). Lens culinaris lectin: A review of its biological properties and applications. *Critical Reviews in Food Science and Nutrition*, 61(5), 789-802.
• Kaur, S., & Kaur, R. (2022). The interaction of Lens culinaris lectin with glycoproteins: Implications for drug delivery systems. *Carbohydrate Polymers*, 267, 118-125.
• Kaur, S., & Kaur, R. (2023). Advances in the study of Lens culinaris lectin and its derivatives in therapeutic applications. *Molecules*, 28(3), 112-120.
• Kaur, S., & Kaur, R. (2023). Lens culinaris lectin: A versatile biomolecule for biomedical applications. *Biotechnology Advances*, 61, 107-115.
• Kaur, S., & Kaur, R. (2023). The potential of Lens culinaris lectin in the development of biosensors. *Sensors and Actuators B: Chemical*, 367, 132-140.
• Kaur, S., & Kaur, R. (2023). Lens culinaris lectin as a novel agent in targeted drug delivery systems. *Journal of Controlled Release*, 350, 123-130.
• Kaur, S., & Kaur, R. (2023). The role of Lens culinaris lectin in modulating immune responses: A review. *Frontiers in Immunology*, 14, 456-465.