Jones Reagent (Highly Pure) – Catalog Number: B2012852 (25 mL)

Jones Reagent (Highly Pure) is supplied as a biotechnology-grade solution in a defined 25 mL fill (Catalog B2012852) with storage specified at 2–8 °C. As described by the supplier, the reagent is a solution of chromium trioxide in aqueous sulfuric acid and is intended as a molecular tool for various biochemical applications. In classical synthetic workflows, Jones reagent is widely used for the oxidation of primary alcohols and secondary alcohols under straightforward conditions, providing a practical option when a strongly oxidizing, chromic acid–type medium is appropriate. 

The liquid, ready-to-use format reduces variability associated with in-house preparation and supports rapid method development: researchers can titrate the reagent into their chosen solvent system, monitor qualitative endpoints, and document performance windows without the added steps of compounding and standardizing fresh solutions. Because the MD listing does not declare a working concentration, good practice is to establish conditions empirically for each substrate class—recording solvent, temperature history, and quench/neutralization details so outcomes are reproducible across runs and operators. The concise specification set (amount, supplied-as, storage, and keyword identity) simplifies SOP inclusion and inventory traceability, while the biotechnology-grade note (Type I ultrapure water; 0.22 um filtration for solutions) aligns with expectations for low background during optimization experiments.

Typical research scenarios include small-scale substrate screens to compare oxidation behavior, preparation of control materials for qualitative checks, and workflow benchmarking where a robust, well-characterized oxidant is required. When integrating into new protocols, it is sensible to run a short series of pilot reactions to map selectivity and to confirm compatibility with co-solvents, functional groups, and downstream workup steps. Within these research-only boundaries, this clearly specified Jones reagent offering provides a dependable, easy-to-document component for laboratories standardizing alcohol oxidation procedures in exploratory chemical and biochemical contexts.

Why researchers choose this product:

• Defined 25 mL fill in a ready-to-use solution format for consistent method setup
• Listed as chromium trioxide in aqueous sulfuric acid; widely used to oxidize primary and secondary alcohols
• Specified storage at 2–8 °C supports routine cold-chain handling
• Biotechnology-grade preparation (Type I water; 0.22 um filtration for solutions)
• Clear keyword identity (e.g., “Jone′s reagent”) aids searchability and SOP alignment

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Jones LJ, Yue ST, Cheung CY, Singer VL. RNA quantitation by fluorescence-based solution assay: RiboGreen reagent characterization Anal Biochem. 1998 Dec 15;265(2):368-74.
• 2: Aricescu AR, Lu W, Jones EY. A time- and cost-efficient system for high-level protein production in mammalian cells Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1243-50.
• 3: Compton SJ, Jones CG. Mechanism of dye response and interference in the Bradford protein assay Anal Biochem. 1985 Dec;151(2):369-74.
• 4: Chung EM, Pavio M. Pediatric Extranodal Lymphoma Radiol Clin North Am. 2016 Jul;54(4):727-46.
• 5: Jones DH. Panhandle PCR PCR Methods Appl. 1995 Apr;4(5):S195-201.
• 6: PLoS Medicine Editors. Speed and convenience aren’t everything with diagnostics PLoS Med. 2011 Oct;8(10):e1001113.
• 7: Solaja BA, Milić DR, Dosen-Mićović LI. Oxidation of steroidal 5-en-3 beta-ols with Jones reagent in ether Steroids. 1994 May;59(5):330-4.
• 8: Jones LM. Mass spectrometry-based methods for structural biology on a proteome-wide scale Biochem Soc Trans. 2020 Jun 30;48(3):945-954.
• 9: Gilroy S, Bethke PC, Jones RL. Calcium homeostasis in plants J Cell Sci. 1993 Oct;106 ( Pt 2):453-61.
• 10: Davies JS, Enjalbal C, Wise C, Jones G, Handa BK. Further studies on cyclic RGD analogues Biochem Soc Trans. 1994 Nov;22(4):1049-52. pubmed.ncbi.nlm.nih.gov