Immobilized DNase I – Research Use Only

Immobilized DNase I is a high-purity, matrix-bound deoxyribonuclease I supplied as a 1 mL suspension (Catalog #B2016385). The enzyme is covalently immobilized on agarose beads, allowing easy removal after use, repeated application, and reduced contamination of samples with free enzyme. It is an excellent tool for complete removal of genomic DNA from RNA preparations, elimination of plasmid DNA in protein samples, prevention of viscosity in cell lysates, and many other nucleic acid decontamination workflows.

Scientific Overview

DNase I is an endonuclease that nonspecifically cleaves single- and double-stranded DNA, producing 5'-phosphorylated oligonucleotides. When immobilized on a solid support, the enzyme retains its activity while offering major practical advantages: it can be easily removed by centrifugation or filtration, reused in multiple cycles, and prevents carry-over of free nuclease into sensitive downstream applications. Immobilized DNase I is particularly valuable for RNA purification (removal of genomic DNA contamination), protein sample cleanup, and viscosity reduction in cell lysates without introducing soluble enzyme that could interfere with subsequent steps.

This Immobilized DNase I is suitable for:

• Complete removal of DNA from RNA preparations
• Elimination of plasmid or genomic DNA from protein samples
• Reduction of viscosity in bacterial or mammalian cell lysates
• Reusable DNA decontamination workflows
• Prevention of DNA contamination in sensitive molecular biology assays

Usage & Handling Guidance

Store at room temperature (RT). Before use, gently resuspend the immobilized enzyme. Add an appropriate amount of the suspension to your sample and incubate with gentle mixing (typically 15–60 min at room temperature or 37 °C). Remove the immobilized enzyme by centrifugation or filtration. The support can often be washed and reused.

• Recommended applications: DNA removal from RNA or protein samples, lysate viscosity reduction
• Typical usage: 10–50 µL of suspension per mL of sample (optimize for complete DNA digestion)
• Stability: Stable at room temperature; avoid extreme pH or high salt during storage
• Handling: Resuspend gently before pipetting; use in DNase-free tubes

What You Get

• 1 mL Immobilized DNase I suspension
• Ready-to-use matrix-bound deoxyribonuclease for efficient DNA removal
• Biotechnology-grade preparation with high enzymatic activity
• Convenient format that allows easy removal and potential reuse
• For research use only (RUO)

Why Researchers Choose It

• Immobilized format allows easy removal by centrifugation or filtration, preventing carry-over contamination
• Highly effective for removing DNA from RNA preparations and protein samples
• Reduces lysate viscosity without introducing soluble nuclease
• Reusable in many protocols, offering cost-effectiveness and consistency
• Biotechnology-grade quality ensures reliable performance in sensitive workflows

Frequently Asked Questions (FAQ)

• What is the advantage of immobilized DNase I?
  The enzyme can be easily removed after digestion, preventing contamination of the sample with free nuclease.
• What is it primarily used for?
  Removal of genomic or plasmid DNA from RNA preps, protein samples, and reduction of lysate viscosity.
• How do I use the product?
  Resuspend gently, add to your sample, incubate, then remove the beads by centrifugation or filtration.
• Can the immobilized enzyme be reused?
  Yes, in many protocols the support can be washed and reused several times.
• How should I store the product?
  Store at room temperature (RT) as recommended.

References

• Du Y, Chen Y, Li F, Mao Z, Ding Y, Wang W. Genetically Engineered Cellular Nanovesicle as Targeted DNase I Delivery System for the Clearance of Neutrophil Extracellular Traps in Acute Lung Injury. Adv Sci (Weinh). 2023 Nov;10(32):e2303053.
• Wang CJ, Ko GR, Lee YY, Park J, Park W, Park TE, Jin Y, Kim SN, Lee JS, Park CG. Polymeric DNase-I nanozymes targeting neutrophil extracellular traps for the treatment of bowel inflammation. Nano Converg. 2024 Feb 8;11(1):6.
• Ye J, Shao C, Zhang X, Guo X, Gao P, Cen Y, Ma S, Liu Y. Effects of DNase I coating of titanium on bacteria adhesion and biofilm formation. Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:738-747.
• Sato S, Fujita K, Kanazawa M, Mukumoto K, Ohtsuka K, Takenaka S. Reliable ferrocenyloligonucleotide-immobilized electrodes and their application to electrochemical DNase I assay. Anal Chim Acta. 2009 Jul 10;645(1-2):30-5.
• Rittich B, Spanová A, Ohlashennyy Y, Lenfeld J, Rudolf I, Horák D, Benes MJ. Characterization of deoxyribonuclease I immobilized on magnetic hydrophilic polymer particles. J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Jul 5;774(1):25-31.
• Krasnorutskii MA, Buneva VN, Nevinsky GA. Immunization of rabbits with DNase I produces polyclonal antibodies with DNase and RNase activities. J Mol Recognit. 2008 Jul-Aug;21(4):233-42.
• Zhang Y, Ying JY. Homogeneous Immunochemical Assay on the Lateral Flow Strip for Measurement of DNase I Activity. Anal Chem. 2015 Oct 20;87(20):10193-8.
• Yuan Z, Lin C, Dai L, He Y, Hu J, Xu K, Tao B, Liu P, Cai K. Near-Infrared Light-Activatable Dual-Action Nanoparticle Combats the Established Biofilms of Methicillin-Resistant Staphylococcus aureus and Its Accompanying Inflammation. Small. 2021 Apr;17(13):e2007522.
• Lu M, Guo Q, Seeman