Human Hemopexin Antibody – Research Use Only

Human Hemopexin Antibody is a high-affinity polyclonal antibody supplied as 1.0 mg in solution (Catalog #B2011269, concentration >4.5 mg/mL). Hemopexin is the primary plasma protein responsible for binding free heme with extremely high affinity, thereby preventing heme-induced oxidative damage and facilitating safe heme recycling in the liver. This antibody is an excellent tool for detecting and quantifying hemopexin in human samples, studying heme metabolism, acute-phase responses, oxidative stress, and inflammation-related pathways.

Scientific Overview

Hemopexin is a 60 kDa plasma glycoprotein that binds free heme with the highest known affinity of any protein. By sequestering heme, it prevents heme-catalyzed formation of reactive oxygen species and lipid peroxidation while delivering heme to hepatocytes for safe catabolism via heme oxygenase-1. Hemopexin levels increase during the acute-phase response to inflammation, infection, or tissue injury, making it both a protective factor and a potential biomarker. This polyclonal antibody enables sensitive detection and quantification of human hemopexin in serum, plasma, and tissues.

This Human Hemopexin Antibody is suitable for:

• Western blot and ELISA quantification of hemopexin in human samples
• Immunohistochemistry and immunofluorescence localization in tissues
• Studies of heme metabolism, oxidative stress, and acute-phase responses
• Investigation of hemopexin as a biomarker in inflammation and hemolytic conditions
• Research on heme scavenging and protection against heme toxicity

Usage & Handling Guidance

Store at 2–8 °C. For long-term storage, aliquot and freeze at –20 °C or below. Typical working dilutions are 1:1000–1:10,000 for Western blot and 1:200–1:2000 for ELISA (optimize for your specific system). Centrifuge briefly before use if any precipitate is observed.

• Recommended applications: WB, ELISA, IHC, and IF
• Working dilution: Optimize empirically (commonly 1:1000–1:10,000 for WB; 1:200–1:2000 for ELISA)
• Stability: Stable at 2–8 °C for short term; aliquot and freeze for long-term storage
• Handling: Use in a clean environment; validate specificity with appropriate controls

What You Get

• 1.0 mg Human Hemopexin Antibody in solution (>4.5 mg/mL)
• High-affinity polyclonal antibody for sensitive detection of human hemopexin
• Biotechnology-grade reagent suitable for multiple immunoassay techniques
• Convenient quantity for multiple experiments and protocol optimization
• For research use only (RUO)

Why Researchers Choose It

• High-affinity antibody specific for human hemopexin
• Excellent for quantifying hemopexin as an acute-phase reactant and heme scavenger
• Useful in studies of oxidative stress, inflammation, hemolysis, and tissue injury
• High concentration (>4.5 mg/mL) allows flexible dilution for different applications
• Reliable performance in Western blot, ELISA, IHC, and related techniques

Frequently Asked Questions (FAQ)

• What does this antibody detect?
  It specifically recognizes human hemopexin, the primary plasma heme-binding protein.
• What are the main applications?
  Western blot, ELISA, immunohistochemistry, and immunofluorescence for hemopexin detection and quantification.
• What dilution should I start with?
  Typical starting dilutions are 1:1000–1:10,000 for Western blot and 1:200–1:2000 for ELISA. Optimize for your system.
• How should I store the antibody?
  Store at 2–8 °C for short-term use. For long-term storage, aliquot and freeze at –20 °C or below.
• Is this antibody suitable for rat or mouse samples?
  It is raised against human hemopexin; cross-reactivity with rodent hemopexin should be validated experimentally.

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References

• Winter NA, Gibson PG, Fricker M, Simpson JL, Wark PA, McDonald VM. Hemopexin: A Novel Anti-inflammatory Marker for Distinguishing COPD From Asthma. Allergy Asthma Immunol Res. 2021 May;13(3):450-467.
• Bakker WW, Melgert BN, Faas MM. Hemopexin: anti-inflammatory, pro-inflammatory, or both? J Leukoc Biol. 2010 Jan;87(1):1-2; author reply 3, 5.
• Ingoglia G, Sag CM, Rex N, De Franceschi L, Vinchi F, Cimino J, Petrillo S, Wagner S, Kreitmeier K, Silengo L, Altruda F, Maier LS, Hirsch E, Ghigo A, Tolosano E. Data demonstrating the anti-oxidant role of hemopexin in the heart. Data Brief. 2017 May 13;13:69-76.
• Vandooren J, Van den Steen PE, Opdenakker G. Biochemistry and molecular biology of gelatinase B or matrix metalloproteinase-9 (MMP-9): the next decade. Crit Rev Biochem Mol Biol. 2013 May-Jun;48(3):222-72.
• Franklin JL, Bennett WL, Messina JL. Insulin attenuates TNFα-induced hemopexin mRNA: An anti-inflammatory action of insulin in rat H4IIE hepatoma cells. Biochem Biophys Rep. 2017 Jan 5;9:211-216.
• Fink MP. Editorial: Hemopexin: newest member of the anti-inflammatory mediator club. J Leukoc Biol. 2009 Aug;86(2):203-4.
• Mauk MR, Smith A, Mauk AG. An alternative view of the proposed alternative activities of hemopexin. Protein Sci. 2011 May;20(5):791-805.
• Liu W, Lu L, Pan H, He X, Zhang M, Wang N, Zhu J, Yi H, Tang S. Heme oxygenase-1 and hemopexin gene polymorphisms and the risk of anti-tuberculosis drug-induced hepatotoxicity in China. Pharmacogenomics. 2022 May;23(7):431-441.
• Dong BB, Zhu FY, Wei HD, Dong HL, Xiong LZ. [Progress in biochemical characteristics of hemopexin and its clinical application]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Apr;21(2):513-6.
• Yalamanoglu A, Dubach IL, Schulthess N, Ingoglia G, Swindle DC, Humar R, Schaer DJ, Buehler PW, Irwin DC, Vallelian F. Agonistic Anti-CD40 Antibody Triggers an Acute Liver Crisis With Systemic Inflammation in Humanized Sickle Cell Disease Mice. Front Immunol. 2021 Mar 4;12:627944.