Human CIRBP Protein – Research Use Only

Human CIRBP Protein is a biotechnology-grade RNA-binding protein reagent supplied as a 100 µg solution at 0.5 mg/mL for research applications involving RNA biology, stress-response signaling, protein–RNA interaction studies, and biochemical assay development. CIRBP (Cold Inducible RNA-Binding Protein), also known as CIRP, is widely studied for its role in cellular stress adaptation, RNA regulation, inflammation-associated pathways, and gene expression control. This ready-to-use protein solution is well suited for laboratories investigating RNA-binding proteins, cellular stress mechanisms, and protein function in molecular and biochemical systems.

Scientific Overview

CIRBP is a cold-inducible RNA-binding protein involved in the cellular response to environmental and physiological stress. It has been widely studied for its role in RNA stability, transcriptional and post-transcriptional regulation, stress granule biology, inflammatory signaling, and cellular adaptation pathways.

In molecular biology and protein research, CIRBP is commonly used to investigate RNA–protein interactions, stress-regulated gene expression, protein phase behavior, and pathway-level responses to cellular stress conditions. Its relevance across inflammation, cancer biology, RNA regulation, and stress signaling makes it a useful tool for a broad range of mechanistic laboratory studies.

Applications

• Protein–RNA interaction and RNA-binding studies
• Stress-response and cellular adaptation research
• Biochemical assay development involving RNA regulatory proteins
• Inflammation- and signaling-related mechanistic studies
• Molecular biology workflows involving RNA processing and protein function

Usage & Handling Guidance

Store at -20°C as specified. Because this product is supplied in solution, aliquoting is recommended where appropriate to reduce repeated freeze–thaw exposure. Working concentration and assay conditions should be optimized according to the intended experimental system, especially in workflows involving RNA binding or protein interaction studies.

• Preparation: Use directly or dilute in an appropriate buffer for the intended assay
• Handling: Minimize repeated freeze–thaw cycles to help preserve protein integrity
• Optimization: Adjust concentration based on assay design and interaction conditions
• Workflow note: Validate performance within the intended molecular or biochemical system

What You Get

• 100 µg of Human CIRBP Protein supplied at 0.5 mg/mL
• Ready-to-use solution format for convenient experimental setup
• Biotechnology-grade protein reagent for RNA and stress biology workflows
• Suitable for protein interaction and mechanistic molecular studies
• For research use only (RUO)

Why Researchers Choose It

• Relevant to RNA biology, cellular stress, and inflammation-related research
• Useful for protein–RNA interaction and mechanistic pathway studies
• Solution format supports convenient assay integration
• Applicable to molecular biology, protein chemistry, and signaling workflows
• Suitable for exploratory and hypothesis-driven laboratory studies

Frequently Asked Questions (FAQ)

• What is CIRBP used for in research?
  CIRBP is commonly used in studies involving RNA binding, stress-response signaling, gene regulation, inflammation-associated pathways, and molecular interaction analysis.
• What does CIRBP stand for?
  CIRBP stands for Cold Inducible RNA-Binding Protein, also commonly referred to as CIRP.
• What form is this product supplied in?
  This product is supplied as a protein solution at 0.5 mg/mL, allowing convenient use in biochemical and molecular biology workflows.
• How should this protein be handled after opening?
  Aliquoting is generally recommended to minimize repeated freeze–thaw cycles and help preserve protein quality during long-term use.
• Can this product be used for therapeutic or diagnostic purposes?
  No. This product is supplied strictly for research use only and is not intended for diagnostic or therapeutic use.

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References

• Lujan DA, Ochoa JL, Hartley RS. Cold-inducible RNA binding protein in cancer and inflammation. Wiley Interdiscip Rev RNA. 2018 Mar;9(2):e1462.
• Kim YM, Hong S. Controversial roles of cold-inducible RNA-binding protein in human cancer (Review). Int J Oncol. 2021 Nov;59(5):91.
• Aziz M, Brenner M, Wang P. Extracellular CIRP (eCIRP) and inflammation. J Leukoc Biol. 2019 Jul;106(1):133-146.
• Robichaud S, Fairman G, Vijithakumar V, Mak E, Cook DP, Pelletier AR, Huard S, Vanderhyden BC, Figeys D, Lavallée-Adam M, Baetz K, Ouimet M. Identification of novel lipid droplet factors that regulate lipophagy and cholesterol efflux in macrophage foam cells. Autophagy. 2021 Nov;17(11):3671-3689.
• Lu M, Ge Q, Wang G, Luo Y, Wang X, Jiang W, Liu X, Wu CL, Xiao Y, Wang X. CIRBP is a novel oncogene in human bladder cancer inducing expression of HIF-1α. Cell Death Dis. 2018 Oct 12;9(10):1046.
• Chongsathidkiet P, Fecci PE. Cold-inducible RNA-binding protein (CIRBP) as a biomarker to predict recurrence of brain metastases. Neuro Oncol. 2021 Sep 1;23(9):1419-1420.
• Gokhale NS, McIntyre ABR, Mattocks MD, Holley CL, Lazear HM, Mason CE, Horner SM. Altered m(6)A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection. Mol Cell. 2020 Feb 6;77(3):542-555.e8.
• Hozumi H, Kataoka K, Kondoh Y, Isayama T, Okada J, Sugiura K, Mori K, Kono M, Suzuki Y, Karayama M, Furuhashi K, Enomoto N, Fujisawa T, Inui N, Nakamura Y, Suda T. Clinical Significance of Cold-Inducible RNA-Binding Protein in Idiopathic Pulmonary Fibrosis. Chest. 2021 Dec;160(6):2149-2157.
• Zhou Q, Usluer S, Zhang F, Lenard AJ, Bourgeois BMR, Madl T. ATP regulates RNA-driven cold inducible RNA binding protein phase separation. Protein Sci. 2021 Jul;30(7):1438-1453.