Refill low-range nickel testing without changing chemistry.
Choose HI93740-01 for 50 tests or HI93740-03 for 150 tests. This mixed powder-and-liquid PAN reagent family replenishes an established nickel low range photometer workflow. The larger pack increases test capacity, not measuring range; these are not 100- or 300-test packs.
Manufacturer’s generic reagent-packet photograph, not the full mixed powder/liquid set or an itemized contents image. Printed 09/2022 date is illustrative, not the expiration of supplied stock.
Match the instrument, method and reporting basis
Use the HI97740 nickel low range method, reporting 0.000 to 1.000 mg/L as Ni. Match the nickel parameter and low-range PAN procedure: this is not an iron reagent or an interchangeable high-range nickel refill.
PAN chemistry with a prepared blank
An adaptation of the 1-(2-pyridylazo)-2-naphthol (PAN) method develops an orange tint. A reacted deionized-water blank and a separately reacted sample are both required; an untreated sample is not the method blank.
Reagent consumption per complete test
HI93740A-0 and HI93740C-0 powders are used with HI93740B-0 liquid. Each complete determination consumes two A packets, 2 mL B and two C packets in total, shared across a 25 mL deionized-water blank and a separate 25 mL sample preparation.
Check the dispersant before testing
HI93703-51 is not a routine addition to the nickel method. If turbidity develops above 30 °C, the HI97740 manual calls for two to three drops per cuvette before zeroing and reading. Confirm the selected pack’s contents and your dispersant supply if that treatment is needed.
Follow the measurement sequence
Prepare 25 mL deionized water and 25 mL sample in separate graduated beakers. Add one A packet to each and dissolve fully, especially if ferric iron is present. Add 1 mL B to each, mix and allow the 15-minute reaction before adding one C packet to each and dissolving. Transfer 10 mL of the prepared blank and reacted sample to separate cuvettes, zero with the blank and read the sample. Treat turbidity as directed before zeroing.
Prepare the rest of the setup
Have a compatible photometer, clean cuvettes with caps and stoppers, deionized water and suitable volume-measuring equipment available separately. The nickel procedure also needs two graduated beakers. CAL Check standards verify the instrument; they do not replace sample-reaction reagents. Plan pack use around the supplied lot’s expiration date, repeat measurements and quality-control needs.
Check the sample matrix
The nickel method lists matrix restrictions, including chloride above 8000 mg/L and ferric iron above 10 mg/L; cobalt and ferrous iron must not be present. Dissolve reagent A completely when ferric iron is present. Check all listed limits in the manual rather than assuming suitability for every mixed-metal or seawater sample.
Frequently Asked Questions
Which pack size should I order?
HI93740-01 provides 50 tests; HI93740-03 provides 150 tests. Order quantity 1 for one selected sellable pack. Capacity refers to complete determinations, not the number of individual powder packets or bottles.
Which instrument and mode are verified?
Use the HI97740 nickel low range method, reporting 0.000 to 1.000 mg/L as Ni. Match the nickel parameter and low-range PAN procedure: this is not an iron reagent or an interchangeable high-range nickel refill.
What chemistry and units does the method use?
An adaptation of the 1-(2-pyridylazo)-2-naphthol (PAN) method develops an orange tint. A reacted deionized-water blank and a separately reacted sample are both required; an untreated sample is not the method blank. The HI97740 method range is 0.000 to 1.000 mg/L as Ni.
How much reagent is used per determination?
HI93740A-0 and HI93740C-0 powders are used with HI93740B-0 liquid. Each complete determination consumes two A packets, 2 mL B and two C packets in total, shared across a 25 mL deionized-water blank and a separate 25 mL sample preparation.
Is dispersing agent needed, and is it included?
HI93703-51 is not a routine addition to the nickel method. If turbidity develops above 30 °C, the HI97740 manual calls for two to three drops per cuvette before zeroing and reading. Confirm the selected pack’s contents and your dispersant supply if that treatment is needed.
What is the blank and reaction sequence?
Prepare 25 mL deionized water and 25 mL sample in separate graduated beakers. Add one A packet to each and dissolve fully, especially if ferric iron is present. Add 1 mL B to each, mix and allow the 15-minute reaction before adding one C packet to each and dissolving. Transfer 10 mL of the prepared blank and reacted sample to separate cuvettes, zero with the blank and read the sample. Treat turbidity as directed before zeroing.
Can other metals or sample salts affect the reading?
The nickel method lists matrix restrictions, including chloride above 8000 mg/L and ferric iron above 10 mg/L; cobalt and ferrous iron must not be present. Dissolve reagent A completely when ferric iron is present. Check all listed limits in the manual rather than assuming suitability for every mixed-metal or seawater sample.
What should I have ready before opening a pack?
Have a compatible photometer, clean cuvettes with caps and stoppers, deionized water and suitable volume-measuring equipment available separately. The nickel procedure also needs two graduated beakers. CAL Check standards verify the instrument; they do not replace sample-reaction reagents. Plan pack use around the supplied lot’s expiration date, repeat measurements and quality-control needs. Review the component-specific US Safety Data Sheets and the instrument method manual before handling or disposal. This listing is reagent replenishment, not a promise that every startup item is supplied.
