Keep the conditional ozone correction ready.
Hanna HI93703-52 supplies pre-measured glycine powder for the additional ozone procedure when chlorine is present or suspected. The 100-test pack is a separate optional helper for compatible benchtop photometers—not a standalone ozone assay and not a routine addition to chlorine-free samples. Keep it available where chlorine interference is a realistic part of the testing workflow, alongside the separate HI93757 ozone reagents.
Hanna’s generic powder-reagent reference photo supplied for HI93703-52. It does not show a glycine-specific label or establish physical packet inventory. Printed expiry is illustrative only.
Match the refill to the complete method
Use HI93703-52-0 glycine only for the additional reading in the documented ozone procedure. HI83326 is the primary reference here; HI83300 and HI83399 also document the conditional procedure. The ozone assay range belongs to the instrument method, not to glycine as a separate measurement.
A helper with a specific job
The glycine step belongs inside a two-reading ozone correction. Having a separate refill avoids confusing an optional interference-handling material with the ozone color reagent itself. HI93703-52 is the orderable pack; the instrument method identifies its powder as HI93703-52-0.
Decide from the sample, not from habit
If free or total chlorine is present or suspected, follow the additional procedure in your exact-model ozone manual. Chlorine-free samples use the standard ozone procedure without glycine. A routine glycine addition is not a substitute for identifying the relevant sample conditions or for performing the required standard reading.
Record A, prepare a fresh portion, then record B
When free or total chlorine is present or suspected, first perform the standard ozone measurement and record A. On a fresh 10 mL sample, zero again, add one HI93703-52-0 glycine packet and dissolve completely, then add one HI93757-0 ozone packet. Shake gently for 20 seconds and wait 2 minutes before reading B. Report ozone as A − B, not B alone.
Budget for ozone reagent as well
The completed correction consumes two HI93757-0 ozone portions and one glycine packet. This glycine pack is labeled 100 tests, but it contains neither the separate HI93757 ozone assay refill nor a photometer. The 100/300 nominal ozone pack labels must not be read as 100/300 corrected results, and nominal test labels alone do not establish an exact physical packet inventory.
Keep the reaction order and timing intact
For the additional reading on HI83326, dissolve glycine completely before adding the ozone reagent. After the ozone packet, shake gently for 20 seconds and wait 2 minutes or use the instrument timer. Report A − B. Do not report the additional reading alone as the corrected ozone result or improvise a different mixing sequence.
The correction does not replace matrix checks
Bromine, chlorine dioxide and iodine interfere. Above 500 mg/L hardness as CaCO3, the HI83326 manual calls for approximately 2 minutes of shaking after powder addition. Alkalinity above 250 mg/L as CaCO3 can prevent full color development or cause fading; follow the manual’s dilute-HCl neutralization guidance.
Prepare the complete setup
Provide a compatible photometer, suitable clean cuvettes with caps and stoppers, HI93757 ozone reagent and any other sample-treatment materials called for by the method. Check received lot/expiry markings and read the glycine US Safety Data Sheet. The linked ozone instrument manuals explain its legitimate procedural context; there is no separate glycine instrument assay being offered here.
Frequently Asked Questions
Is HI93703-52 a standalone ozone test?
No. It is an optional glycine helper pack. The ozone determination still requires separate HI93757-0 ozone reagent and a compatible photometer, plus the complete standard and additional measurement procedure.
When should I use it?
Only when free or total chlorine is present or suspected in the ozone sample, as directed by the instrument’s ozone method. It is not routinely required for chlorine-free water.
What do HI93703-52 and HI93703-52-0 mean?
HI93703-52 is the orderable 100-test glycine pack. HI93703-52-0 is the glycine powder component code printed in the ozone method and in its US Safety Data Sheet.
How do I obtain the corrected ozone result?
When free or total chlorine is present or suspected, first perform the standard ozone measurement and record A. On a fresh 10 mL sample, zero again, add one HI93703-52-0 glycine packet and dissolve completely, then add one HI93757-0 ozone packet. Shake gently for 20 seconds and wait 2 minutes before reading B. Report ozone as A − B, not B alone.
How much reagent does one corrected result consume?
Two ozone-reagent portions and one glycine packet: ozone reagent for A and ozone reagent plus glycine for B. The ozone reagent is sold separately. Do not treat the ozone pack’s nominal test count as the corrected-result count.
Can I change the order or shorten the wait?
Follow the manual: dissolve glycine completely on the fresh zeroed portion, add ozone reagent, shake gently for 20 seconds and wait 2 minutes or use the timer. Do not replace that sequence with an assumed chemical masking mechanism.
Which instrument manuals support this use?
The HI83326, HI83300 and HI83399 Ozone sections identify optional HI93703-52-0 glycine and the A − B correction. They are instrument method references, not standalone glycine manuals.
Does glycine resolve every ozone interference?
Bromine, chlorine dioxide and iodine interfere. Above 500 mg/L hardness as CaCO3, the HI83326 manual calls for approximately 2 minutes of shaking after powder addition. Alkalinity above 250 mg/L as CaCO3 can prevent full color development or cause fading; follow the manual’s dilute-HCl neutralization guidance. Follow all matrix precautions; this helper is for the specified chlorine-containing procedure, not a universal sample treatment.
