G2 Peptide – Research Use Only

Scientific Overview

G2 Peptide is a synthetic glycopeptide featuring a complex N‑linked biantennary glycan (Galβ1‑4GlcNAcβ1‑2Manα1‑3[Galβ1‑4GlcNAcβ1‑2Manα1‑6]Manβ1‑4GlcNAcβ1‑4GlcNAc) covalently attached to a peptide backbone. This structure closely resembles naturally occurring N‑glycans found on many mammalian glycoproteins, making the G2 Peptide a valuable tool for studying glycan–protein interactions, receptor recognition, enzymatic processing, and glycosylation‑dependent biological mechanisms.

Due to its defined glycan architecture and high purity, the G2 Peptide is widely used in glycobiology, structural biology, immunology, and biochemical assay development. It serves as a model substrate for glycosidases, glycosyltransferases, and lectin binding studies, as well as a reference standard for analytical method development.

Common Research Applications

• Glycan–protein interaction studies
• Lectin binding assays
• Glycosidase and glycosyltransferase substrate research
• Structural and functional glycobiology
• Analytical method development (LC‑MS, HPLC)
• Peptide‑based biochemical assays

Usage & Handling Guidance

This product is supplied as a lyophilized powder. For experimental use, researchers typically reconstitute the G2 Peptide in sterile water, dilute acetic acid, or buffer depending on assay requirements. Glycopeptides may require gentle warming or sonication to fully dissolve. Aliquoting after reconstitution is recommended to prevent degradation from repeated freeze–thaw cycles.

• Reconstitution: Sterile water, dilute acetic acid, or assay‑compatible buffer
• Storage: −20 °C (powder); −80 °C recommended for long‑term storage of solutions
• Handling: Avoid repeated freeze–thaw cycles

What You Get

• 1 mg high‑purity G2 glycopeptide
• Defined N‑linked glycan structure
• Stable powder form for flexible experimental use
• For research use only (RUO)

Frequently Asked Questions

• Is this product suitable for clinical or diagnostic use? No. This product is for research use only.
• Can I obtain a Certificate of Analysis (COA) or Technical Data Sheet (TDS)? COA and TDS availability depends on the manufacturer’s current active batch. In many cases, documentation can be provided upon request. Please contact us to inquire about documentation for the specific lot being shipped.
• How should I reconstitute this glycopeptide? Sterile water, dilute acetic acid, or assay‑specific buffers are commonly used.
• Is this product suitable for lectin or glycosidase assays? Yes. The G2 glycan structure is frequently used in enzymatic and binding studies.

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References

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• Aldughaim MS, Alsaffar F, Barker MD. Coupling of a Novel TIMP3 Peptide to Carboxypeptidase G2 for Pro-Drug Activation at the Tumour Site. Molecules. 2021 Jan 25;26(3):625.
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• Hu YX, Liu Z, Zhang Z, Deng Z, Huang Z, Feng T, Zhou QH, Mei S, Yi C, Zhou Q, Zeng PH, Pei G, Tian S, Tian XF. Antihepatoma peptide, scolopentide, derived from the centipede scolopendra subspinipes mutilans. World J Gastroenterol. 2023 Mar 28;29(12):1875-1898.
• Okada J, Yamada E, Saito T, Okada K, Yamada M, Ohshima K, Okada S. FAM83G-based Peptide Induces Apoptosis on Cultured Liver Cancer Cell. Protein Pept Lett. 2022;29(12):1082-1087.
• Tang Y, Xu H, Dai Y, Wang F, Huang W, Liu P, Gu Y. A novel peptide targeting c-Met for hepatocellular carcinoma diagnosis. J Mater Chem B. 2021 Jun 2;9(22):4577-4586.