Collagenase D Enzyme – Research Use Only

Collagenase D Enzyme (Catalog #B2016642) is a high-purity collagenase derived from Clostridium histolyticum, supplied as a 50 mg lyophilized powder. This biotechnology-grade enzyme is optimized for selective degradation of collagen in the extracellular matrix (ECM), making it an essential tool for tissue dissociation, cell isolation, and matrix remodeling studies.

Scientific Overview

Collagenase D is a purified collagenase preparation from Clostridium histolyticum that specifically targets native collagen in the extracellular matrix. It is widely used for gentle yet efficient dissociation of tissues rich in collagen (such as skin, lung, liver, and tumors) while preserving cell viability. Unlike crude collagenase mixtures, Collagenase D offers a more defined activity profile, making it suitable for reproducible cell isolation, matrix remodeling studies, and preparation of single-cell suspensions for downstream applications including flow cytometry, primary cell culture, and single-cell sequencing.

This Collagenase D Enzyme is suitable for:

• Enzymatic dissociation of collagen-rich tissues
• Isolation of primary cells (fibroblasts, epithelial cells, hepatocytes, etc.)
• Studies of extracellular matrix degradation and remodeling
• Preparation of tissue samples for proteomics or histological analysis
• Biochemical assays involving collagen turnover

Usage & Handling Guidance

Store the lyophilized powder at 2–8 °C. Reconstitute in sterile ultrapure water or appropriate buffer (e.g., PBS or HBSS) to the desired concentration immediately before use. Typical working concentrations range from 0.5–2 mg/mL depending on tissue type and desired dissociation time. Incubate at 37 °C with gentle agitation. Prepare fresh working solutions for best results.

• Recommended applications: Tissue dissociation and ECM degradation
• Working concentration: 0.5–2 mg/mL (optimize for tissue type and incubation time)
• Stability: Lyophilized form is stable at 2–8 °C; reconstituted enzyme should be used the same day
• Handling: Use sterile techniques; avoid repeated freeze–thaw of reconstituted solutions

What You Get

• 50 mg Collagenase D Enzyme as lyophilized powder
• High-purity collagenase from Clostridium histolyticum optimized for ECM degradation
• Biotechnology-grade reagent with low non-specific proteolytic activity
• Convenient format for reproducible tissue dissociation protocols
• For research use only (RUO)

Why Researchers Choose It

• Highly pure Collagenase D with strong collagenolytic activity
• Effective for dissociation of dense, collagen-rich tissues while preserving cell viability
• Lyophilized format ensures long-term stability and flexibility in reconstitution
• Biotechnology-grade quality provides consistent lot-to-lot performance
• Ideal for primary cell isolation, ECM studies, and downstream single-cell applications

Frequently Asked Questions (FAQ)

• What is Collagenase D used for?
  It is primarily used for enzymatic dissociation of collagen-rich tissues and isolation of primary cells.
• How do I reconstitute the powder?
  Dissolve in sterile PBS, HBSS, or ultrapure water to the desired concentration (typically 0.5–2 mg/mL). Use immediately or prepare single-use aliquots.
• What tissues is it best suited for?
  It performs well on skin, lung, liver, adipose, and tumor tissues where collagen is abundant.
• Can it be used for single-cell RNA sequencing?
  Yes, when used under optimized gentle dissociation conditions to preserve cell viability and RNA integrity.
• How should I store the product?
  Store the lyophilized powder at 2–8 °C. Avoid repeated freeze–thaw of reconstituted enzyme.

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