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Biomiga Lentivirus Concentration Reagent V2001 product packaging
Biomiga Lentivirus Concentration Reagent V2001: Buffer LP and Buffer LS, 50–100-fold concentration and about 60–70% recovery per Biomiga, 3,000 rpm spins with no ultracentrifuge
Five-step lentivirus concentration workflow: clarify and filter supernatant, add Buffer LP 1:3 at 4 °C, pellet at 3,000 rpm, resuspend in Buffer LS, aliquot at −80 °C
V2001 pack sizes by supernatant capacity: 10 mL treats up to 30 mL, 100 mL up to 300 mL, 500 mL up to 1.5 L, 2,000 mL up to 6 L
V2001 kit contents, Buffer LP and Buffer LS, and what the user supplies: supernatant, 0.45 µm filter, refrigerated centrifuge, BSL-2 workspace
Biomiga Lentivirus Concentration Reagent V2001 product packaging
Biomiga Lentivirus Concentration Reagent V2001: Buffer LP and Buffer LS, 50–100-fold concentration and about 60–70% recovery per Biomiga, 3,000 rpm spins with no ultracentrifuge
Five-step lentivirus concentration workflow: clarify and filter supernatant, add Buffer LP 1:3 at 4 °C, pellet at 3,000 rpm, resuspend in Buffer LS, aliquot at −80 °C
V2001 pack sizes by supernatant capacity: 10 mL treats up to 30 mL, 100 mL up to 300 mL, 500 mL up to 1.5 L, 2,000 mL up to 6 L
V2001 kit contents, Buffer LP and Buffer LS, and what the user supplies: supernatant, 0.45 µm filter, refrigerated centrifuge, BSL-2 workspace

Biomiga Lentivirus Concentration Reagent, No Ultracentrifuge (V2001)

$24.00

    Biomiga's Lentivirus Concentration Reagent concentrates recombinant lentivirus from transfected-cell culture supernatant without ultracentrifugation. Mix one volume of Buffer LP with three volumes of clarified supernatant, chill, pellet at 3,000 rpm, and resuspend in Buffer LS. Biomiga reports 50–100-fold concentration with about 60–70% recovery.

    • Low-speed workflow: a refrigerated benchtop centrifuge replaces the ultracentrifuge
    • Up to 30 mL of supernatant (1–2 T75 flasks) per prep; scale by pack size
    • Both buffers store at room temperature (15–25 °C)

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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50–100×
Biomiga-stated concentration
~60–70%
Biomiga-stated recovery
30 mL
Supernatant per prep
15–25 °C
Buffer storage

LENTIVIRUS CONCENTRATION

Concentrate lentivirus on a benchtop centrifuge, not an ultracentrifuge.

Ultracentrifugation has been the traditional way to separate lentivirus from cellular proteins and media, but Biomiga notes it is time-consuming and limits how much material you can process. The Biomiga Lentivirus Concentration Reagent (V2001) concentrates recombinant lentivirus from transfected-cell culture supernatant with two buffers and low-speed spins. Biomiga reports the virus can be concentrated 50–100-fold, with a recovery rate of around 60–70%. Biomiga states the virus is stable in Buffer LP, so the incubation can run overnight.

For research use only. Not for use in humans. All steps require at least Biosafety Level 2 conditions.

How it works

Lentivirus concentration workflow with Biomiga V2001: clarify, add Buffer LP, pellet at 3,000 rpm, resuspend in Buffer LS, store at −80 °C

Conditions from Biomiga's instructions for use; up to 30 mL of supernatant per prep.

STEP 1

Clarify

Spin the culture media 10 min at 3,000 rpm, 4 °C, and pass the supernatant through a 0.45 µm filter. Clarified supernatant can be stored at −80 °C until you're ready.

STEP 2

Add Buffer LP

Mix 1 volume of Buffer LP with 3 volumes of supernatant (for example, 5 mL into 15 mL). Incubate at 4 °C for at least 4 hours or overnight.

STEP 3

Pellet

Spin 30 min at 3,000 rpm, 4 °C. Remove all the supernatant; the pellet may look hazy. Keep it on ice.

STEP 4

Resuspend and store

Dissolve the pellet in 300–500 µL Buffer LS, spin 2 min at 8,000 rpm, and transfer the supernatant to a clean vial. Aliquot and store at −80 °C.

Protocol at a glance

Step Speed / temperature Time
Clarify 3,000 rpm, 4 °C, then 0.45 µm filter 10 min
Buffer LP incubation 4 °C 4 h to overnight
Pellet 3,000 rpm, 4 °C 30 min
Resuspend and clear 300–500 µL Buffer LS; 8,000 rpm, 4 °C 2 min
Store −80 °C —

Which pack do I need?

Which V2001 pack to choose: supernatant capacity for each pack size, three times the Buffer LP volume

Supernatant capacity is 3 × the Buffer LP volume, from Biomiga's 1 : 3 ratio.

Pack Buffer LP Buffer LS Supernatant it can treat
V2001-00 10 mL 1 mL up to 30 mL
V2001-01 100 mL 10 mL up to 300 mL
V2001-02 500 mL 50 mL up to 1.5 L
V2001-03 2,000 mL included up to 6 L
TRIAL SIZE

V2001-00 · 10 mL

One full 30 mL prep, or a first trial on your own supernatant.

V2001-01 · 100 mL

Regular small-scale production.

V2001-02 · 500 mL

Large or pooled harvests.

V2001-03 · 2,000 mL

Core facilities and scale-up.

BEFORE YOU START

You supply

Clarified virus supernatant, 0.45 µm filters, sterile conical and microcentrifuge tubes, a refrigerated centrifuge (3,000 rpm), a microcentrifuge (8,000 rpm, 4 °C), cryovials and a BSL-2 workspace.

In the kit

Buffer LP: added at 1 volume per 3 volumes of supernatant.

Buffer LS: 300–500 µL per prep to resuspend the virus pellet.

Volumes for each pack are in the table above.

Built for

Transduction stocks

Raise titer before infecting hard-to-transduce cells.

Labs without an ultracentrifuge

A refrigerated benchtop centrifuge is enough.

Viral vector cores

Scale up with the 500 mL and 2,000 mL packs.

When this reagent is the right call

  • No ultracentrifuge access: low-speed spins at 3,000 rpm.
  • Overnight-friendly: Biomiga states the virus is stable in Buffer LP, so the incubation can run overnight.
  • Simple storage: both buffers store at room temperature.
  • Try before scaling: the 10 mL pack treats one full 30 mL prep.

Related Biomiga kits

EndoFree ezFilter Plasmid Maxiprep Kit (PD1522)

Endotoxin-free plasmid prep for the transfection step upstream.

Lentivirus Purification Mini and Maxi Kits (V1170, V1270)

Biomiga's lentivirus purification kits. Ask us for details.

Need one of the others? Email info@bluetigerscientific.com or text (833) 928-4437 for a quote.

Frequently asked questions

Quick facts

What it is: A two-buffer reagent set for concentrating recombinant lentivirus from culture supernatant without ultracentrifugation.

Ratio: 1 volume Buffer LP to 3 volumes supernatant.

Per prep: up to 30 mL supernatant; 300–500 µL Buffer LS.

Pack sizes: 10, 100, 500 and 2,000 mL.

Use: In vitro research use only, under BSL-2 conditions.

How much does it concentrate the virus?

Biomiga reports 50–100-fold concentration with a recovery rate of around 60–70%. Results depend on your construct, packaging system and harvest.

Do I need an ultracentrifuge?

No. Biomiga's protocol uses a refrigerated centrifuge at 3,000 rpm and a microcentrifuge at 8,000 rpm, both at 4 °C. Biomiga gives speeds in rpm, so check your rotor's g-force.

How much supernatant can I process?

Up to 30 mL per prep, about 1–2 T75 flasks. Each prep takes one third of its volume in Buffer LP, so 30 mL of supernatant needs 10 mL. Across a whole pack, the supernatant you can treat is three times the Buffer LP volume.

How long does it take?

Plan for an incubation of at least 4 hours, or overnight, at 4 °C, plus about 45 minutes of spins. Clarified supernatant can be stored at −80 °C before you start.

How should the reagent and the virus be stored?

Store both buffers at room temperature (15–25 °C). Aliquot the concentrated virus and store it at −80 °C. The expiry date is on the label.

What biosafety level is required?

Biomiga warns that lentivirus-containing media and concentrated virus can be infectious to humans and animals. All steps must be performed under at least Biosafety Level 2 conditions.

How does it compare with Takara's Lenti-X Concentrator?

Both use 1 volume of reagent per 3 volumes of clarified, 0.45 µm-filtered supernatant, followed by a low-speed spin at 4 °C instead of ultracentrifugation. Takara's published protocol calls for 30 minutes to overnight at 4 °C and a 1,500 × g spin for 45 minutes. Biomiga specifies at least 4 hours to overnight and 30 minutes at 3,000 rpm. We don't compare recovery between the two products; validate on your own supernatant, and the 10 mL pack is sized for a trial. Lenti-X is a trademark of Takara Bio Inc.; Blue Tiger Scientific is not affiliated with Takara Bio.

Can it be used for clinical or in-human work?

No. It is for in vitro research use only and not for use in humans.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

    SpecificationDetail
    ManufacturerBiomiga, Inc. (San Diego, CA)
    Catalog NumberV2001-00 (10 mL), V2001-01 (100 mL), V2001-02 (500 mL), V2001-03 (2,000 mL)
    ComponentsBuffer LP; Buffer LS
    Contents by PackV2001-00: Buffer LP 10 mL, Buffer LS 1 mL; V2001-01: LP 100 mL, LS 10 mL; V2001-02: LP 500 mL, LS 50 mL; V2001-03: 2,000 mL size, Buffer LP and Buffer LS
    SampleRecombinant lentivirus in transfected-cell culture supernatant, clarified and 0.45 µm filtered
    Input per PrepUp to 30 mL supernatant (1–2 T75 flasks)
    Mixing Ratio1 volume Buffer LP : 3 volumes supernatant
    Concentration / Recovery50–100-fold; around 60–70% recovery (Biomiga-stated)
    Centrifugation3,000 rpm at 4 °C; 8,000 rpm at 4 °C for final clearing
    Resuspension300–500 µL Buffer LS
    StorageStore all components at room temperature (15–25 °C); store concentrated virus at −80 °C
    ShippingShips at ambient temperature
    BiosafetyPerform all steps under at least BSL-2 conditions
    Country of OriginUSA
    HazardousConsult the current manufacturer SDS and supplied instructions for buffer hazards, handling, and storage.
    Intended UseIn vitro research use only (RUO); not for use in humans

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

    Payment Terms
    Orders are prepaid by card, or invoiced against an institutional purchase order once credit is approved. Prices are in USD and subject to change without notice. Purchase orders are due within 30 days of invoice unless agreed otherwise in writing.

    Order Confirmation & Cancellations
    Confirmed orders are placed with the manufacturer and are final. Changes or cancellations must be requested in writing before shipment and approved by Blue Tiger Scientific; approved cancellations may incur restocking, materials or third-party fees.

    Accuracy of Orders
    Customers are responsible for verifying catalog numbers, pack sizes, ship-to address and recipient availability before submitting an order.

    Shipping & Cold Chain
    Shipping is calculated at checkout. Temperature-sensitive products ship by expedited service, and ship dates may be adjusted to avoid weekend or holiday transit. Orders placed on weekends or holidays are processed the next business day. Delivery dates are estimates and are not guaranteed. A recipient must be available to receive and store temperature-sensitive products on arrival. Blue Tiger Scientific is not liable for carrier delays, weather disruptions or other events beyond our control.

    Receiving & Claims
    Inspect every shipment on arrival. Shortages, damage or thawed temperature-sensitive product must be reported to Blue Tiger Scientific in writing, with photos, within 2 business days of delivery. Claims received after that window, and shipments that are refused, unclaimed or left unattended, are not eligible for credit or replacement.

    Returns & Refunds
    Returns require a Return Merchandise Authorization (RMA) issued by Blue Tiger Scientific and must be requested within 30 days of delivery. Refunds or replacements are issued only for shipping errors or verified product defects. Temperature-sensitive products are not returnable once delivered. Customers pay return shipping unless the error was ours, and refunds exclude original shipping charges. Approved refunds are processed within 45 business days.

    Warranty
    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

    Limitation of Liability
    Blue Tiger Scientific's total liability for any claim is limited to the purchase price of the product involved. Blue Tiger Scientific and Biomiga are not liable for indirect, incidental or consequential damages, including lost research time or materials, arising from the use or misuse of any product.

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    Handle all products according to the Safety Data Sheet and the manufacturer's instructions, using appropriate personal protective equipment.

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    Customers must use products in compliance with all applicable local, state, federal and international laws, including export control and biosafety regulations. Products may not be exported or re-exported without the required authorizations.

    Resale
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    Force Majeure
    Neither party is liable for delays or failures caused by events beyond its reasonable control, including weather, carrier disruptions, supply shortages or changes in regulation.

    Governing Law
    These terms are governed by the laws of the State of California and the United States.

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