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Biomiga EndoFree ezFilter Plasmid Maxiprep Kit PD1522
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit PD1522
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)
Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)

Biomiga EndoFree ezFilter Plasmid Maxiprep Kit, Endotoxin-Free (PD1522)

$45.00

    Endotoxin-free plasmid maxiprep kit that purifies transfection-grade plasmid DNA from 150-400 mL of E. coli culture, with a syringe filter for fast lysate clearing.

    • Endotoxin below 0.1 EU per µg of plasmid DNA, removed during purification
    • Up to 1,200 µg binding capacity per maxi column; 700-1,000 µg typical from 200 mL of a pUC plasmid
    • 60 mL ezFilter syringe clears lysate in about 5 minutes; runs on a vacuum manifold or centrifuge
    • Elutes in 1.5-2 mL, ready for endotoxin-sensitive cell lines, primary cells and microinjection

    A practical alternative to endotoxin-free maxi kits from QIAGEN and other major brands.

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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<0.1 EU/µg
Endotoxin in purified DNA
1,200 µg
Binding capacity per column
150–400 mL
E. coli culture per prep
~5 min
Lysate clearing by syringe filter

WHY ENDOTOXIN-FREE

Clean plasmid for the cells that notice.

Plasmid made with a standard prep carries bacterial endotoxin (lipopolysaccharide) from the E. coli outer membrane. Robust lines like HEK293 tolerate it, but endotoxin-sensitive cell lines, primary cells and microinjection work call for it to be removed first. The Biomiga EndoFree ezFilter Plasmid Maxiprep Kit (PD1522) strips endotoxin inside the purification itself and delivers plasmid below 0.1 EU per µg, eluted directly in 1.5 to 2 mL with no precipitation step.

How it works

STEP 1

Lyse and clear

Resuspend, lyse and neutralize, then push the lysate through the 60 mL ezFilter syringe. About five minutes instead of a 20-minute high-speed spin.

STEP 2

Strip endotoxin and bind

Endotoxin-removal buffer and ethanol go into the cleared lysate, which then loads onto the maxi column. No phase-partition extraction.

STEP 3

Wash and dry

Wash the column and dry it, on a vacuum manifold or in a centrifuge.

STEP 4

Elute

Elute in 1.5 to 2 mL of EndoFree Elution Buffer and reapply once for a second pass.

Full volumes, speeds and the centrifuge-only protocol are in the user manual.

Expected yield

Yield depends on the plasmid's origin of replication, its size and the host strain. Biomiga's figures per 200 mL of culture:

Plasmid Origin Copy number Yield (µg / 200 mL)
pUC Mutated pMB1 500–700 700–1,000
pBluescript ColE1 300–500 450–600
pGEM Mutated pMB1 300–400 350–450
pBR322 pMB1 15–20 35–50
pSuperCos pMB1 10–20 30–50
pACYC P15A 10–12 25–40
pSC101 pSC101 5 12

Getting the most plasmid

Do

  • Use an endA- host such as DH5α or TOP10
  • Grow 12 to 16 hours in LB to an OD600 of 2.0 to 3.0
  • Resuspend the pellet completely before lysis
  • Warm Buffer B1 to redissolve any precipitate, and keep its cap tight
  • Scale culture and buffers up 2 to 3 times for low-copy plasmids
  • Dry the column fully before eluting

Don't

  • Overload the column: biomass (OD600 x mL) is optimal at 450 to 550
  • Vortex after adding B1 or lyse longer than 5 minutes
  • Hold culture at 4 °C overnight; freeze the pellet at -20 °C instead
  • Forget to add RNase A to Buffer A1
  • Use rich media above OD600 3.0

Working with an endA+ strain such as HB101 or JM101? Ask us about Biomiga PD1713.

EZFILTER VS. STANDARD MAXI

Why the ezFilter version?

Clearing the lysate is the slowest, most equipment-hungry step of a maxiprep. The ezFilter syringe turns it into a five-minute bench step, so you don't need a high-speed rotor for clearing or wait out a 20-minute spin. With a vacuum manifold, several preps run side by side.

You supply: 100% ethanol, 50 mL conical tubes, and a centrifuge reaching 5,000 x g or a vacuum manifold with a 200 to 600 mbar pump.

What's in the box (25 preps)

  • 25 ezBind maxi columns
  • 25 filter syringes, 60 mL
  • Buffers A1 and B1, 270 mL each
  • Buffer N3, 80 mL
  • Buffer RET, 2 x 250 mL
  • Buffers ER (13 mL) and D1 (27 mL)
  • EndoFree Elution Buffer, 135 mL
  • RNase A, 20 mg/mL, 1.35 mL
  • User manual

The 2-prep trial and 10-prep sizes contain the same components scaled down.

Built for labs where endotoxin matters

Primary cell work

Transfect sensitive primary cultures without endotoxin in the prep.

Sensitive cell lines

For lines that respond to endotoxin, where HEK293-grade preps fall short.

Microinjection

Endotoxin-free plasmid for injection work.

Core facilities

Run several maxipreps in parallel on a vacuum manifold.

Which Biomiga plasmid kit do I need?

THIS KIT

EndoFree ezFilter Maxi (PD1522)

Endotoxin-free, 150–400 mL culture, fastest lysate clearing.

EndoFree ezFlow Maxi (PD1520)

Same endotoxin-free maxi scale, without the syringe filter.

EndoFree ezFlow Midi (PD1420)

Endotoxin-free DNA at midi scale for smaller cultures.

Plasmid ezFilter Maxi (PD1512)

When endotoxin doesn't matter: cloning, mapping, sequencing.

Need one of the others? Email info@bluetigerscientific.com or text (833) 928-4437 for a quote.

How it compares

Feature Biomiga PD1522 QIAGEN EndoFree Plasmid Maxi
Chemistry ezBind DNA binding column Anion-exchange resin
Processing Vacuum or centrifuge Gravity flow
Lysate clearing 60 mL ezFilter syringe QIAfilter cartridge
Endotoxin removal Integrated (Buffer RET) Integrated
Stated endotoxin Below 0.1 EU/µg Below 0.04 EU/µg

Specifications as published by each manufacturer. QIAGEN and EndoFree are trademarks of QIAGEN; Blue Tiger Scientific is not affiliated with QIAGEN.

When this kit is the right call

  • Bigger cultures: accepts 150 to 400 mL per prep, with headroom for low-copy plasmids.
  • Your equipment, your way: vacuum manifold or centrifuge, no gravity-flow waiting.
  • Ready to use: direct elution in 1.5 to 2 mL, no alcohol precipitation.
  • Try before you stock up: a 2-prep trial size lets you validate it on your own plasmid first.

If your application needs the lowest possible endotoxin, compare stated levels above and ask us before ordering.

Frequently asked questions

Quick facts

What it is: A maxiprep kit that purifies endotoxin-free plasmid DNA from 150–400 mL of E. coli culture.

Best for: Transfection of endotoxin-sensitive cell lines and primary cells, and microinjection.

Alternative to: QIAGEN EndoFree Plasmid Maxi and similar endotoxin-free maxi kits.

Output: Up to 1,000 µg of a high-copy plasmid from 200 mL, below 0.1 EU/µg endotoxin.

Pack sizes: 2 preps (trial), 10 preps and 25 preps.

Use: In vitro research use only.

Is this an alternative to the QIAGEN EndoFree Plasmid Maxi Kit?

Yes, for labs that want endotoxin-free plasmid at maxi scale. Both build endotoxin removal into the prep. The Biomiga kit uses a binding column on a vacuum manifold or centrifuge and elutes directly; the QIAGEN kit uses anion-exchange chromatography under gravity flow. QIAGEN states a lower endotoxin level (below 0.04 EU/µg versus below 0.1 EU/µg).

How much plasmid DNA will I get?

Biomiga lists 700 to 1,000 µg from 200 mL of culture for a high-copy pUC plasmid, and less for lower-copy vectors. See the yield table above.

How long does the prep take?

Biomiga positions its EndoFree ezFlow line as a prep of under 60 minutes, not counting the overnight culture. Times vary with the protocol and the number of preps.

Do I need a vacuum manifold?

No. The manual includes a centrifuge-only protocol. A manifold makes it easier to run several preps at once.

Can I use it for low-copy plasmids?

Yes. Scale the culture volume and the buffer volumes up 2 to 3 times, as Biomiga recommends.

Is the DNA suitable for primary cells?

Biomiga designed the endotoxin-free DNA for transfection of endotoxin-sensitive cell lines, primary cultured cells and microinjection.

How should the kit be stored?

At room temperature (22 to 25 °C), except Buffer A1 after RNase A is added and Buffer ER, which go to 4 °C. Shelf life is guaranteed for 12 months from purchase.

Can this kit be used for clinical or in vivo human use?

No. It is intended for in vitro research use only and is not for use in humans.

Can my institution get a formal quote?

Yes. Email info@bluetigerscientific.com or text (833) 928-4437 for a formal quote your purchasing department can process. Blue Tiger Scientific is a DVBE-certified supplier.

    SpecificationDetail
    Catalog NumberPD1522-00 (2 preps), PD1522-01 (10 preps), PD1522-02 (25 preps)
    FormatMaxi column with 60 mL syringe filter
    Starting Material150-400 mL E. coli culture (optimal biomass OD600 x mL of 450-550)
    Binding Capacity1,200 µg per column
    Endotoxin LevelBelow 0.1 EU per µg plasmid DNA
    Typical Yield700-1,000 µg from 200 mL (pUC, high copy)
    Elution Volume1.5-2 mL EndoFree Elution Buffer
    ProcessingVacuum manifold (200-600 mbar) or centrifuge
    Centrifuge Requirement5,000 x g; 10,000 rpm for centrifuge-only lysate clearing
    Shipping and StorageShips at ambient temperature. Store at room temperature; Buffer A1 (with RNase A) and Buffer ER at 4 °C
    Shelf Life12 months from purchase (guaranteed)
    Country of OriginUSA
    HazardousNo (Buffers N3 and RET contain acetic acid; wear gloves and eye protection)
    Intended UseIn vitro research use only (RUO); not for use in humans

    Terms & Conditions

    Blue Tiger Scientific is an independent distributor of products manufactured by Biomiga, Inc. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms.

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnostic procedures, or therapy, and may not be resold to consumers. Sales are limited to verified institutional and business customers shipping to a business address; we do not ship to residential addresses. Blue Tiger Scientific may request institutional verification or end-use information before shipping and may cancel any order that cannot be verified.

    Payment Terms
    Orders are prepaid by card, or invoiced against an institutional purchase order once credit is approved. Prices are in USD and subject to change without notice. Purchase orders are due within 30 days of invoice unless agreed otherwise in writing.

    Order Confirmation & Cancellations
    Confirmed orders are placed with the manufacturer and are final. Changes or cancellations must be requested in writing before shipment and approved by Blue Tiger Scientific; approved cancellations may incur restocking, materials or third-party fees.

    Accuracy of Orders
    Customers are responsible for verifying catalog numbers, pack sizes, ship-to address and recipient availability before submitting an order.

    Shipping & Cold Chain
    Shipping is calculated at checkout. Temperature-sensitive products ship by expedited service, and ship dates may be adjusted to avoid weekend or holiday transit. Orders placed on weekends or holidays are processed the next business day. Delivery dates are estimates and are not guaranteed. A recipient must be available to receive and store temperature-sensitive products on arrival. Blue Tiger Scientific is not liable for carrier delays, weather disruptions or other events beyond our control.

    Receiving & Claims
    Inspect every shipment on arrival. Shortages, damage or thawed temperature-sensitive product must be reported to Blue Tiger Scientific in writing, with photos, within 2 business days of delivery. Claims received after that window, and shipments that are refused, unclaimed or left unattended, are not eligible for credit or replacement.

    Returns & Refunds
    Returns require a Return Merchandise Authorization (RMA) issued by Blue Tiger Scientific and must be requested within 30 days of delivery. Refunds or replacements are issued only for shipping errors or verified product defects. Temperature-sensitive products are not returnable once delivered. Customers pay return shipping unless the error was ours, and refunds exclude original shipping charges. Approved refunds are processed within 45 business days.

    Warranty
    Biomiga warrants its products to perform as described in their labeling when used in accordance with their instructions; the remedy for a product that does not is replacement, at Biomiga's option. Blue Tiger Scientific coordinates these claims with Biomiga. No other warranties, express or implied, are given.

    Limitation of Liability
    Blue Tiger Scientific's total liability for any claim is limited to the purchase price of the product involved. Blue Tiger Scientific and Biomiga are not liable for indirect, incidental or consequential damages, including lost research time or materials, arising from the use or misuse of any product.

    Safety
    Handle all products according to the Safety Data Sheet and the manufacturer's instructions, using appropriate personal protective equipment.

    Legal & Export Compliance
    Customers must use products in compliance with all applicable local, state, federal and international laws, including export control and biosafety regulations. Products may not be exported or re-exported without the required authorizations.

    Resale
    Products may not be resold or redistributed without prior written approval from Blue Tiger Scientific.

    Force Majeure
    Neither party is liable for delays or failures caused by events beyond its reasonable control, including weather, carrier disruptions, supply shortages or changes in regulation.

    Governing Law
    These terms are governed by the laws of the State of California and the United States.

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