Beta-Galactosidase Chromogenic Substrate Buffer – Catalog Number: B2010650 (50 mL)

Beta-Galactosidase Chromogenic Substrate Buffer is supplied as a biotechnology-grade solution in a defined 50 mL fill (Catalog B2010650) with storage at −20 C. The supplier describes this reagent as an optimized buffer for the specific stabilization of beta-galactosidase chromogenic substrates, and notes its usage as a stabilizing agent for beta-galactosidase-related applications. In development and QC workflows where β-galactosidase is used for qualitative color formation, the longevity and consistency of chromogenic substrates are critical to signal reliability, background control, and day-to-day reproducibility. A dedicated stabilization buffer helps protect substrate integrity during storage and handling, supporting consistent color development once the enzyme reaction is initiated.

The concise specification set—amount, supplied-as, storage, keyword identity, and biotechnology-grade note—facilitates SOP documentation and inventory traceability. Since no working concentration is prescribed, a practical approach is to confirm buffer compatibility with your specific substrate and assay matrix through small pilot runs. Record the substrate identity, buffer-to-substrate ratios, incubation times, and temperature history along with lot identifiers; these records streamline method transfer and help standardize performance across operators and instruments. Because the product is delivered as a ready-to-use solution, it integrates quickly into benchtop workflows that screen alternative chromogenic substrates or compare incubation parameters (e.g., temperature or pH windows) for color development.

Common research scenarios include reporter gene readouts using β-galactosidase, senescence-associated β-galactosidase staining formats, and general enzyme detection schemes that rely on chromogenic conversion. In each case, substrate stability before use and during setup can influence background levels and dynamic range. Supported by the biotechnology-grade preparation statement (solutions prepared using Type I ultrapure water and fine filtration), this buffer offers a clearly specified option for laboratories standardizing β-galactosidase chromogenic work in research-only settings.

Why researchers choose this product:

• Formulated to stabilize beta-galactosidase chromogenic substrates before assay setup
• Ready-to-use solution in a defined 50 mL fill simplifies method integration
• Specified storage at −20 C supports consistent shelf-life management
• Biotechnology-grade note (Type I water; 0.22 µm filtration for solutions)
• Concise documentation fields (amount, supplied-as, storage, keywords) streamline SOP/LIMS mapping

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Itahana K, Campisi J, Dimri GP. Methods to detect biomarkers of cellular
  senescence: the senescence-associated beta-galactosidase assay. Methods Mol
  Biol. 2007;371:21-31.
• 2: Juers DH, Matthews BW, Huber RE. LacZ β-galactosidase: structure and function
  of an enzyme of historical and molecular biological importance. Protein Sci.
  2012 Dec;21(12):1792-807.
• 3: Matsuyama K, Kondo T, Igarashi K, Sakamoto T, Ishimaru M. Substrate-
  recognition mechanism of tomato β-galactosidase 4 using X-ray crystallography
  and docking simulation. Planta. 2020 Oct 3;252(4):72.
• 4: Aburto C, Castillo C, Cornejo F, Arenas-Salinas M, Vásquez C, Guerrero C,
  Arenas F, Illanes A, Vera C. β-Galactosidase from Exiguobacterium acetylicum:
  Cloning, expression, purification and characterization. Bioresour Technol. 2019
  Apr;277:211-215.
• 5: Hosseini A, Mas J. The β-galactosidase assay in perspective: Critical
  thoughts for biosensor development. Anal Biochem. 2021 Dec 15;635:114446.
• 6: Spring SA, Goggins S, Frost CG. Ratiometric electrochemical detection of
  β-galactosidase. Org Biomol Chem. 2017 Aug 30;15(34):7122-7126.
• 7: Gong H, Zhang B, Little G, Kovar J, Chen H, Xie W, Schutz-Geschwender A,
  Olive DM. beta-Galactosidase activity assay using far-red-shifted fluorescent
  substrate DDAOG. Anal Biochem. 2009 Mar 1;386(1):59-64.
• 8: Mine M, Mizuguchi H, Takayanagi T. Kinetic analysis of substrate competition
  in enzymatic reactions with β-D-galactosidase by capillary electrophoresis /
  dynamic frontal analysis. J Pharm Biomed Anal. 2020 Sep 5;188:113390.
• 9: Sharma SK, Li S, Micic M, Orbulescu J, Weissbart D, Nakahara H, Shibata O,
  Leblanc RM. β-Galactosidase Langmuir Monolayer at Air/X-gal Subphase Interface.
  J Phys Chem B. 2016 Dec 8;120(48):12279-12286.
• 10: Carneiro LABC, Yu L, Dupree P, Ward RJ. Characterization of a β-galactosidase from Bacillus subtilis with transgalactosylation activity. Int J Biol Macromol. 2018 Dec;120(Pt A):279-287. pubmed.ncbi.nlm.nih.gov