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Beta-galactosidase Antibody (Chicken Polyclonal) Antibodies Molecular Depot
Beta-galactosidase Antibody (Chicken Polyclonal) Antibodies Molecular Depot

Beta-galactosidase Antibody (Chicken Polyclonal)

$975.00

    Catalog Number: B2010658 (0.1 mg)


    Beta-galactosidase Antibody (Chicken Polyclonal) is a highly pure antibody specific to the Beta Galactosidase of E. coli. This product has been used as molecular tool in the research and development of bacterial beta-galactosidases. It has also been used in a wide array of other biochemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Beta-galactosidase Antibody (Chicken Polyclonal) – Catalog Number: B2010658 (0.1 mg)

Catalog number: B2010658
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 0.1 mg
Molecular Weight or Concentration: 1 mg/mL
Supplied as: Solution
Applications: molecular tool in the research and development of bacterial beta-galactosidase
Storage: -20C
Keywords: b-gal antibody, anti-b-gal
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Beta-galactosidase Antibody (Chicken Polyclonal) is provided as a biotechnology-grade solution in a defined 0.1 mg fill (Catalog B2010658), with a listed concentration of 1 mg/mL and storage at -20C. The supplier notes this antibody is specific to the E. coli beta-galactosidase and is used as a molecular tool in the research and development of bacterial beta-galactosidases, as well as in a wide array of biochemical and immunological applications. The concise specification set—amount, concentration, supplied-as, storage, keywords, and grade—supports straightforward SOP documentation and inventory traceability for labs standardizing β-gal–related workflows.

In research settings, an anti–β-galactosidase antibody is commonly leveraged to confirm reporter expression, verify enzyme presence in lysates or fixed preparations, or construct capture/detection elements where antibody recognition is the key specificity determinant. Because the listing does not prescribe a working dilution, sensible practice is to establish conditions empirically for the sample type at hand. Small pilot titrations allow teams to balance signal and background while documenting buffer composition, incubation parameters, and time-at-temperature. The solution format reduces variability associated with reconstitution and enables rapid buffer-compatibility checks when adapting the reagent to membrane- or plate-based assays and qualitative imaging workflows.

Practical considerations include selecting blocking and wash buffers that maintain low background with the chosen secondary or detection chemistry, aligning incubation and wash steps with the matrix being tested, and recording lot identifiers to support reproducibility across runs and operators. Within these research-only boundaries, the clearly specified fields and ready-to-dilute format make this chicken polyclonal anti–β-galactosidase a dependable component for laboratories standardizing detection of E. coli β-gal in biochemical and immunological applications.

Why researchers choose this product:

  • Defined 0.1 mg fill at listed 1 mg/mL concentration for precise documentation
  • Ready-to-dilute solution format streamlines pilot titrations and buffer screening
  • Specified storage at -20C supports routine cold-chain handling
  • Targeted to E. coli beta-galactosidase, suitable for reporter and enzyme-detection workflows
  • Biotechnology-grade note (Type I water; 0.22 µm filtration for solutions) aligns with low-background expectations

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • 1: Vinothkumar KR, McMullan G, Henderson R. Molecular mechanism of antibody- mediated activation of β-galactosidase. Structure. 2014 Apr 8;22(4):621-7.
  • 2: Su Z, Gao J, Xie Q, Wang Y, Li Y. Possible role of β-galactosidase in rheumatoid arthritis. Mod Rheumatol. 2020 Jul;30(4):671-680.
  • 3: Kolodych S, Michel C, Delacroix S, Koniev O, Ehkirch A, Eberova J, Cianférani S, Renoux B, Krezel W, Poinot P, Muller CD, Papot S, Wagner A. Development and evaluation of β-galactosidase-sensitive antibody-drug conjugates. Eur J Med Chem. 2017 Dec 15;142:376-382.
  • 4: Welply JK, Fowler AV, Zabin I. beta-Galactosidase alpha-complementation. Overlapping sequences. J Biol Chem. 1981 Jul 10;256(13):6804-10.
  • 5: Ferraz RM, Arís A, Villaverde A. Profiling the allosteric response of an engineered beta-galactosidase to its effector, anti-HIV antibody. Biochem Biophys Res Commun. 2004 Feb 13;314(3):854-60.
  • 6: Sugane K, Sun SH. Detection of anti-helminth antibody by microenzyme-linked immunosorbent assay using recombinant antigen and anti-beta-galactosidase monoclonal antibody. J Immunol Methods. 1994 Jan 12;168(1):55-60.
  • 7: Yu Y, Fang L, Sun D. Biodistribution of HuCC49DeltaCH2-beta-galactosidase in colorectal cancer xenograft model. Int J Pharm. 2010 Feb 15;386(1-2):208-15.
  • 8: Nakano A, Camacho-Alanis F, Ros A. Insulator-based dielectrophoresis with β-galactosidase in nanostructured devices. Analyst. 2015 Feb 7;140(3):860-8.
  • 9: Torgov MY, Alley SC, Cerveny CG, Farquhar D, Senter PD. Generation of an intensely potent anthracycline by a monoclonal antibody-beta-galactosidase conjugate. Bioconjug Chem. 2005 May-Jun;16(3):717-21.
  • 10: Liu Z, Gurlo T, von Grafenstein H. Cell-ELISA using beta-galactosidase conjugated antibodies. J Immunol Methods. 2000 Feb 3;234(1-2):P153-67. pubmed.ncbi.nlm.nih.gov/10669780/

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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