Ames′ Culture Medium – Catalog #B2012459

Ames′ Culture Medium (Catalog #B2012459) is a ready-to-use 1 L liquid medium optimized for the growth of histidine-requiring Salmonella typhimurium tester strains used in the Ames mutagenicity test. This biotechnology-grade medium supports reliable bacterial growth and metabolic activation in mutagenicity and genotoxicity screening assays. It is an essential tool for regulatory safety testing, environmental monitoring, pharmaceutical development, and research into chemical mutagenesis.

Scientific Overview

Ames′ Culture Medium is a specialized nutrient broth formulated for the growth of histidine auxotrophic Salmonella typhimurium strains (TA98, TA100, TA102, TA1535, TA1537, etc.) used in the Ames bacterial reverse mutation test. It supports robust bacterial growth while maintaining the genetic characteristics required for sensitive detection of mutagens. The medium is a cornerstone of regulatory genotoxicity testing (OECD 471) and is widely used in pharmaceutical, chemical, and environmental safety assessment.

Primary uses include:

• Standard Ames mutagenicity testing (with/without metabolic activation)
• Maintenance and propagation of Salmonella tester strains
• Genotoxicity screening of chemicals, pharmaceuticals, and environmental samples
• Research into bacterial mutagenesis mechanisms

Usage & Handling Guidance

Store at 2–8°C. The liquid medium is ready to use. For plate incorporation or pre-incubation methods, follow standard Ames test protocols (e.g., Maron & Ames, 1983). Use within the expiration date and avoid contamination. Warm to room temperature before use if necessary.

• Typical usage: 100 µL per plate in standard Ames test procedures
• Metabolic activation: Compatible with S9 mix for mammalian metabolism simulation
• Sterility: Pre-filtered; maintain aseptic technique during use

What You Get

• 1 L of ready-to-use Ames′ Culture Medium
• Biotechnology-grade formulation optimized for Salmonella tester strains
• Convenient liquid format for immediate use in mutagenicity assays
• For research use only (RUO)

Why Researchers Choose It

• Optimized formulation for reliable growth of Ames tester strains
• Ready-to-use liquid medium simplifies assay setup
• Essential for regulatory-compliant genotoxicity testing (OECD 471)
• High reproducibility and consistent performance
• Proven in thousands of published mutagenicity studies

Frequently Asked Questions (FAQ)

• What is Ames′ Culture Medium used for?
  It is specifically formulated for the Ames bacterial reverse mutation test to detect mutagens and genotoxic compounds.
• Which Salmonella strains is it compatible with?
  It supports the standard histidine-requiring tester strains (TA98, TA100, TA102, TA1535, TA1537, etc.).
• Does it require supplementation?
  It is ready-to-use; add S9 mix for metabolic activation as per protocol.
• How should I store the medium?
  Store at 2–8°C. Use within the expiration date and maintain aseptic technique.
• Is this suitable for research beyond the Ames test?
  Yes. It is useful for general propagation of Salmonella strains and related microbiological studies.

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References

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