Ago1 Antibody – Research Use Only

Ago1 Antibody is a high-affinity polyclonal antibody directed against Argonaute 1 (Ago1, also known as EIF2C1 or GERP95), a core component of the RNA-induced silencing complex (RISC). Supplied as a 10 µg solution with an observed molecular weight of ~100 kDa, this biotechnology-grade antibody is optimized for the detection and study of Ago1 in miRNA-mediated gene silencing, RNA interference (RNAi), and related epigenetic regulatory pathways.

Scientific Overview

Argonaute 1 (Ago1) is a key member of the Argonaute protein family and a central component of the RNA-induced silencing complex (RISC). It binds small non-coding RNAs (miRNAs and siRNAs) to guide sequence-specific gene silencing through mRNA cleavage, translational repression, or chromatin modification. Dysregulation of Ago1 and associated miRNA pathways has been linked to cancer, neurological disorders, and autoimmune conditions, including anti-Ago1 autoantibodies in certain autoimmune neurologic diseases.

This Ago1 Antibody is suitable for:

• Western blotting and immunoprecipitation of endogenous Ago1
• Immunohistochemistry and immunofluorescence for cellular localization
• Studies of miRNA biogenesis, RISC assembly, and gene silencing mechanisms
• Investigation of Ago1 in cancer, autoimmunity, and neurological disorders
• Chromatin immunoprecipitation (ChIP) and related epigenetic assays

Usage & Handling Guidance

Store the antibody solution at -20 °C. Avoid repeated freeze–thaw cycles by preparing single-use working aliquots. Thaw gently on ice and mix by gentle inversion before use. Dilute in appropriate buffer containing carrier protein (e.g., 1–5% BSA or milk) for optimal performance.

• Recommended applications: Western blot, IP, IHC, IF, and RNAi-related studies
• Working dilution: Optimize empirically (typical starting range 1:500 – 1:5,000 for Western blot; adjust for other applications)
• Observed MW: ~100 kDa (may vary slightly with post-translational modifications)
• Handling: Keep on ice during experimental procedures

What You Get

• 10 µg Ago1 Antibody supplied as a solution
• High-specificity reagent targeting human/mammalian Ago1 (~100 kDa)
• Biotechnology-grade antibody suitable for sensitive detection in RNAi and epigenetic research
• Batch-specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Specific detection of Argonaute 1 (Ago1/EIF2C1), a core RISC component
• Useful for studying miRNA-mediated gene silencing and epigenetic regulation
• Supports Western blot, IP, IHC, and immunofluorescence applications
• Relevant for research in cancer, autoimmunity, and neurological disorders
• High-purity biotechnology-grade formulation

Frequently Asked Questions (FAQ)

• What is the observed molecular weight on Western blot?
  Approximately 100 kDa (may show slight variation due to post-translational modifications).
• What applications is this antibody validated for?
  It performs well in Western blotting, immunoprecipitation, immunohistochemistry, and immunofluorescence.
• How should I store the antibody?
  Store at -20 °C. Prepare small working aliquots to minimize freeze–thaw cycles.
• Is this antibody suitable for ChIP assays?
  Yes, it can be used in chromatin immunoprecipitation studies of Ago1-associated complexes.
• Does it cross-react with other Argonaute proteins?
  This antibody is primarily specific for Ago1, though limited cross-reactivity with closely related family members may occur depending on species and conditions.

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References

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• Gupta RK, Abdul-Jawad S, McCoy LE, Mok HP, Peppa D, Salgado M, Martinez-Picado J, Nijhuis M, Wensing AMJ, Lee H, Grant P, Nastouli E, Lambert J, Pace M, Salasc F, Monit C, Innes AJ, Muir L, Waters L, Frater J, Lever AML, Edwards SG, Gabriel IH, Olavarria E. HIV-1 remission following CCR5Δ32/Δ32 haematopoietic stem-cell transplantation Nature. 2019 Apr;568(7751):244-248.
• Moritz CP, Do LD, Tholance Y, Vallayer PB, Rogemond V, Joubert B, Ferraud K, La Marca C, Camdessanché JP, Honnorat J, Antoine JC. Conformation-stabilizing ELISA and cell-based assays reveal patient subgroups targeting three different epitopes of AGO1 antibodies Front Immunol. 2022 Oct 20;13:972161.
• Turchinovich A, Burwinkel B. Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma RNA Biol. 2012 Aug;9(8):1066-75.
• Singh A, Manjunath LE, Kundu P, Sahoo S, Das A, Suma HR, Fox PL, Eswarappa SM. Let-7a-regulated translational readthrough of mammalian AGO1 generates a microRNA pathway inhibitor EMBO J. 2019 Aug 15;38(16):e100727.
• Oliver C, Martinez G. Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis Plant Reprod. 2022 Jun;35(2):153-160.
• Moadab F, Wang X, Najjar R, Ukadike KC, Hu S, Hulett T, Bengtsson AA, Lood C, Mustelin T. Argonaute, Vault, and Ribosomal Proteins Targeted by Autoantibodies in Systemic Lupus Erythematosus J Rheumatol. 2023 Sep;50(9):1136-1144.
• Do LD, Moritz CP, Muñiz-Castrillo S, Pinto AL, Tholance Y, Brugiere S, Couté Y, Stoevesandt O, Taussig MJ, Rogemond V, Vogrig A, Joubert B, Ferraud K, Camdessanché JP, Antoine JC, Honnorat J. Argonaute Autoantibodies as Biomarkers in Autoimmune Neurologic Diseases Neurol Neuroimmunol Neuroinflamm. 2021 Jul 28;8(5):e1032.
• Volpe TA, Demaio J. Chromatin immunoprecipitation in fission yeast Methods Mol Biol. 2011;725:15-28.