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2-Mercaptoethanol 1 M Solution Buffers & Solutions Molecular Depot
2-Mercaptoethanol 1 M Solution Buffers & Solutions Molecular Depot
2-Mercaptoethanol 1 M Solution Buffers & Solutions Molecular Depot
2-Mercaptoethanol 1 M Solution Buffers & Solutions Molecular Depot

2-Mercaptoethanol 1 M Solution

$387.00

    Catalog Number: B2010140 (5 x 1 mL)

    2-Mercaptoethanol 1 M is a ready-to-use 5 × 1 mL biotechnology-grade reducing agent stock (minimum 99% purity). This convenient pre-diluted solution simplifies addition of β-mercaptoethanol to buffers for protein denaturation, disulfide bond reduction, SDS-PAGE sample preparation, and stabilization of thiol-dependent enzymes. Ideal for maintaining reducing conditions in molecular biology, biochemistry, and protein purification workflows. Custom bulk orders of this product are available upon request.

    Products are for in vitro research use only (RUO).

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2-Mercaptoethanol 1 M Solution – Research Use Only

2-Mercaptoethanol 1 M Solution is a ready-to-use, biotechnology-grade reducing agent supplied as 5 × 1 mL aliquots. This pre-diluted 1 M stock simplifies addition of β-mercaptoethanol (BME) to buffers, sample preparations, and enzymatic reactions, providing consistent reducing conditions for protein denaturation, disulfide bond cleavage, and maintenance of thiol-dependent enzyme activity.

Catalog number: B2010140
Lot number: Batch dependent
Expiration Date: Batch dependent
Volume/Weight: 5 × 1 mL
pH: N/A
Supplied as: Ready-to-use solution
Appearance: Clear solution
Applications: Protein reduction and denaturation, SDS-PAGE sample preparation, prevention of disulfide bond formation, stabilization of thiol-dependent enzymes, and redox-sensitive biochemical assays
Storage: 2-8°C
Keywords: ME, βME, Mercaptoethanol, β-Mercaptoethanol
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

Scientific Overview

2-Mercaptoethanol (β-mercaptoethanol, BME) is a small thiol-containing reducing agent that efficiently cleaves disulfide bonds in proteins by thiol-disulfide exchange. The 1 M ready-to-use solution provides a convenient stock for precise addition to buffers and sample preparations, ensuring consistent reducing conditions. It is indispensable in SDS-PAGE sample buffers, protein refolding experiments, prevention of oxidative dimerization, and stabilization of enzymes with essential cysteine residues.

This 2-Mercaptoethanol 1 M Solution is suitable for:

  • Reduction of disulfide bonds prior to electrophoresis or chromatography
  • Maintenance of reducing environments during protein purification and storage
  • Stabilization of thiol-dependent enzymes and prevention of oxidation
  • Sample preparation for mass spectrometry and structural studies
  • General redox biology and biochemical assays requiring controlled reducing conditions

Usage & Handling Guidance

Store the 1 M solution at 2–8 °C. The solution is volatile and has a characteristic strong odor — use in a fume hood when handling concentrated stocks. Dilute to the desired final concentration (commonly 1–5% v/v or 50–700 mM in sample buffers) immediately before use.

  • Recommended applications: SDS-PAGE reducing buffer, protein denaturation, enzyme stabilization, and redox assays
  • Typical working concentration: 50–700 mM final concentration in sample buffers (optimize for specific protocol)
  • Stability: Stable when refrigerated; protect from strong oxidants
  • Handling: Use in a fume hood due to volatility and odor; avoid skin and eye contact

What You Get

  • 5 × 1 mL of 1 M 2-Mercaptoethanol ready-to-use solution
  • Convenient pre-diluted stock for accurate and reproducible addition to buffers
  • High-purity biotechnology-grade reducing agent (minimum 99%)
  • Filtered and prepared with Type I ultrapure water for minimal contamination
  • For research use only (RUO)

Why Researchers Choose It

  • Ready-to-use 1 M stock simplifies accurate dosing and reduces preparation errors
  • Potent reducing agent for cleaving disulfide bonds and maintaining reducing conditions
  • Essential for SDS-PAGE, protein denaturation, and thiol-dependent enzyme stabilization
  • High-purity formulation filtered through 0.22 µm for reliable experimental results
  • Convenient 5 × 1 mL format supports both small-scale and routine laboratory use

Frequently Asked Questions (FAQ)

  • What is the concentration of this solution?
    It is a ready-to-use 1 M (approximately 7.8% v/v) 2-mercaptoethanol solution.
  • What is the typical final concentration in SDS-PAGE sample buffer?
    Commonly 1–5% (v/v) or 50–700 mM final concentration, depending on the protocol.
  • Why does it have a strong odor?
    The characteristic odor is due to the volatility of the thiol compound. Use in a fume hood.
  • How should I store the aliquots?
    Store at 2–8 °C. Once opened, use promptly or keep tightly sealed to minimize oxidation.
  • Is this suitable for mass spectrometry sample preparation?
    Yes, but excess reagent should be removed or quenched prior to MS analysis to avoid interference.
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.

References

  • Click RE. Review: 2-mercaptoethanol alteration of in vitro immune functions of species other than murine. Version 2. J Immunol Methods. 2014 Jan 15;402(1-2):1-8.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

    Legal Use Compliance
    Products must not be used in violation of any law—local, state, federal, or international.

    Accuracy of Orders
    Customers are responsible for verifying all order details before submission.

    Changes or Cancellations
    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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