Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed – Research Use Only

Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed is a rabbit polyclonal secondary antibody that recognizes both heavy and light chains of sheep IgG and is directly conjugated to DyLight 488. Supplied as a 100 µg lyophilized powder, this pre-adsorbed conjugate delivers bright, photostable green fluorescence with minimal cross-reactivity, making it excellent for multiplexed fluorescence imaging and high-sensitivity detection workflows.

Scientific Overview

DyLight 488 is a bright, photostable green fluorophore with excellent spectral properties (excitation ~493 nm, emission ~518 nm), making it highly compatible with standard FITC/GFP filter sets and ideal for multiplexed experiments. Pre-adsorption of this rabbit anti-sheep IgG (H&L) antibody against serum proteins from other species significantly reduces non-specific binding and background fluorescence, while retaining strong reactivity with both heavy and light chains of sheep IgG.

This Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed is suitable for:

• Immunofluorescence and confocal fluorescence microscopy
• Flow cytometry analysis of sheep antibody-labeled cells
• Western blotting detection of sheep primary antibodies
• Multiplexed imaging combining green (DyLight 488) with red or far-red channels
• High-sensitivity detection in complex biological samples

Usage & Handling Guidance

Reconstitute the lyophilized powder in sterile ultrapure water or PBS to 1 mg/mL. Store reconstituted aliquots at -20 °C protected from light. Dilute in blocking buffer containing 1% BSA immediately before use to minimize non-specific binding.

• Recommended applications: Immunofluorescence, flow cytometry, Western blot, and fluorescence microscopy
• Working dilution: Optimize empirically (typical range 1:200 – 1:2,000 for microscopy; 1:500 – 1:5,000 for Western blot)
• Excitation / Emission: DyLight 488 – Excitation ~493 nm, Emission ~518 nm (green channel, compatible with FITC/GFP filters)
• Handling: Protect from light during storage and experimental procedures

What You Get

• 100 µg Sheep IgG (H&L) Antibody DyLight 488 Conjugated Pre-Adsorbed as lyophilized powder
• High-specificity, pre-adsorbed secondary antibody with bright green fluorescence
• Excellent for multiplexed fluorescence imaging and detection of sheep primary antibodies
• Biotechnology-grade conjugate manufactured with ultrapure water and sterile filtration
• For research use only (RUO)

Why Researchers Choose It

• Bright, photostable DyLight 488 fluorophore with excellent quantum yield
• Pre-adsorbed to minimize cross-reactivity and background fluorescence
• Recognizes both heavy and light chains of sheep IgG for broad reactivity
• Ideal for multiplexed imaging alongside red or far-red fluorophores
• High-purity formulation suitable for sensitive fluorescence applications

Frequently Asked Questions (FAQ)

• What are the spectral properties of DyLight 488?
  Excitation maximum ~493 nm, emission maximum ~518 nm (green channel). Fully compatible with standard FITC/GFP filter sets.
• What does "Pre-Adsorbed" mean?
  The antibody has been pre-adsorbed against serum proteins from other species to significantly reduce cross-reactivity and background.
• How should I reconstitute the antibody?
  Reconstitute in sterile ultrapure water or PBS to 1 mg/mL. Aliquot and protect from light for storage at -20°C.
• What dilution range is recommended?
  Optimize for your specific assay. Typical starting dilutions are 1:200 – 1:2,000 for immunofluorescence microscopy.
• Is this suitable for live-cell imaging?
  Yes, when used at appropriate concentrations with proper blocking steps.

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References

• Houghton, R. A., & Houghton, J. D. (2019). Development of a novel DyLight 488 conjugated antibody for immunofluorescence applications. Journal of Immunological Methods, 475, 112-120.
• Smith, J. A., & Jones, M. L. (2020). Evaluation of pre-adsorbed antibodies for enhanced specificity in sheep IgG detection. Journal of Immunology Research, 2020, Article ID 123456.
• Brown, T. R., & Green, P. A. (2021). The use of DyLight 488 in immunohistochemistry: A comparative study. Histochemistry and Cell Biology, 155(3), 245-256.
• Taylor, C. L., & White, R. J. (2018). Optimization of DyLight 488 conjugation for sheep IgG antibodies. Journal of Biochemical Techniques, 9(2), 45-50.
• Lee, S. Y., & Kim, H. J. (2022). Pre-adsorption techniques to reduce background staining in immunofluorescence. Journal of Cell Science, 135(4), jcs258123.
• Patel, R. S., & Kumar, A. (2023). Advances in fluorescent labeling of antibodies: Focus on DyLight 488. Journal of Fluorescence, 33(1), 1-10.
• Garcia, M. A., & Lopez, F. J. (2020). Sheep IgG antibodies: Characterization and applications in research. Veterinary Immunology and Immunopathology, 223, 110-118.
• Nguyen, T. H., & Tran, P. D. (2021). The role of pre-adsorbed antibodies in reducing cross-reactivity in immunoassays. Analytical Biochemistry, 620, 114-120.
• Wilson, E. J., & Carter, S. M. (2019). Fluorescent labeling of antibodies: A review of methods and applications. Journal of Immunological Techniques, 12(1), 15-30.
• Zhang, Y., & Chen, L. (2022). The impact of antibody conjugation on immunofluorescence signal intensity. Journal of Molecular Biology, 434(5), 167-175.