Amine-Modified Polystyrene Latex Beads (5.0-5.9 µm, 5% w/v) – Catalog #B2025677

Amine-Modified Polystyrene Latex Beads (5.0-5.9 µm, 5% w/v) (Catalog #B2025677) are uniform, surface-functionalized 5 µm polystyrene microspheres supplied as a 5 mL 5% aqueous suspension. The primary amine groups on the surface enable easy covalent conjugation to carboxyl-containing molecules (proteins, antibodies, peptides, dyes, etc.) using standard EDC/NHS chemistry. These larger aminated beads are ideal for microscopy, flow cytometry, phagocytosis studies, immunoassays, cell labeling, and the development of custom microparticle-based diagnostic or therapeutic tools.

Scientific Overview

These 5.0–5.9 µm amine-modified polystyrene latex beads feature reactive primary amine groups on the surface, allowing straightforward covalent attachment of carboxyl-containing ligands via EDC/NHS chemistry. The larger micron-scale size makes them particularly suitable for direct microscopic visualization, flow cytometry, phagocytosis assays, and agglutination-based diagnostics. They serve as a versatile platform for creating custom antibody-coated beads, targeted drug-delivery microparticles, and diagnostic reagents.

Key applications include:

• Covalent conjugation of antibodies, proteins, peptides, or dyes
• Phagocytosis and immune cell interaction studies
• Immunoassays and particle-based agglutination tests
• Cell labeling and separation techniques
• Development of targeted microparticles for drug delivery or diagnostics

Usage & Handling Guidance

Store at 2–8°C. Do not freeze. Gently vortex or pipette up and down before use to ensure uniform suspension. For conjugation, wash beads if necessary and follow standard EDC/NHS protocols. After coupling, quench unreacted groups with glycine or ethanolamine.

• Typical working concentration: 0.1–1% (w/v) for conjugation reactions
• Conjugation chemistry: EDC/NHS coupling to carboxyl-containing ligands
• Stability: Stable for months when stored refrigerated; protect from contamination

What You Get

• 5 mL of Amine-Modified Polystyrene Latex Beads (5.0-5.9 µm, 5% w/v suspension)
• Reactive surface amine groups for easy covalent bioconjugation
• Uniform particle size distribution for reproducible results
• Convenient volume for multiple experiments and scale-up
• For research use only (RUO)

Why Researchers Choose It

• High-density amine surface for efficient covalent conjugation
• 5 µm size ideal for microscopy, flow cytometry, and phagocytosis studies
• Stable polystyrene core with low non-specific binding
• Convenient 5% w/v suspension and 5 mL volume for practical lab use
• Versatile platform for custom functionalized microparticles and diagnostics

Frequently Asked Questions (FAQ)

• What chemistry is used for conjugation?
  EDC/NHS coupling to carboxyl groups on proteins, antibodies, or other ligands.
• Can these beads be used in cell studies?
  Yes. The 5 µm size is well-suited for phagocytosis, cell labeling, and interaction assays.
• How should I store the product?
  Store at 2–8°C. Do not freeze. Mix gently before each use.
• Are the beads fluorescent?
  No. These are plain amine-modified polystyrene beads. Fluorescent versions are available separately.
• What is the particle concentration?
  5% (w/v) suspension. Dilute as needed for your conjugation or assay protocol.

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