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Thymidine Phosphorylase, Recombinant from Escherichia coli Proteins Molecular Depot
Thymidine Phosphorylase, Recombinant from Escherichia coli Proteins Molecular Depot

Thymidine Phosphorylase, Recombinant from Escherichia coli

$3,795.00

    Catalog Number: B2019685 (5000 units)

    Thymidine Phosphorylase (TP) is an enzyme that catalyzes the reversible phosphorolysis of thymidine to thymine and ribose-1-phosphate. It plays a crucial role in nucleotide metabolism and recycling of thymidine, essential for DNA synthesis and repair. The enzyme is produced using recombinant DNA technology in Escherichia coli. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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What's Included

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VR Headset

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2 Touch Controllers

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Charging Cable

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Power Adapter

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Thymidine Phosphorylase, Recombinant from Escherichia coli

Catalog number: B2019685
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 5000 units
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: 2-8°C
Keywords: Thymidine:orthophosphate deoxy-D-ribosyltransferase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References

  • Ge C, OuYang L, Ding Q, Ou L. Co-expression of recombinant nucleoside phosphorylase from Escherichia coli and its application Appl Biochem Biotechnol. 2009 Oct;159(1):168-77.
  • Gbaj A, Edwards PN, Reigan P, Freeman S, Jaffar M, Douglas KT. Thymidine phosphorylase from Escherichia coli: tight-binding inhibitors as enzyme active-site titrants J Enzyme Inhib Med Chem. 2006 Feb;21(1):69-73.
  • Karamitros CS, Somody CM, Agnello G, Rowlinson S. Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase Front Bioeng Biotechnol. 2021 Dec 17;9:793985.
  • Moghaddam A, Bicknell R. Expression of platelet-derived endothelial cell growth factor in Escherichia coli and confirmation of its thymidine phosphorylase activity Biochemistry. 1992 Dec 8;31(48):12141-6.
  • Ding Q, Ou L, Wei D, Wei X. Optimum induction of recombinant thymidine phosphorylase and its application Nucleosides Nucleotides Nucleic Acids. 2011 May;30(5):360-8.
  • Carlson CA, Stewart GJ, Ingraham JL. Thymidine salvage in Pseudomonas stutzeri and Pseudomonas aeruginosa provided by heterologous expression of Escherichia coli thymidine kinase gene J Bacteriol. 1985 Jul;163(1):291-5.
  • Moskaleva ND, Sukhodolets VV. [Escherichia coli K-12 mutants for the thymidine phosphorylase structural gene that retain the anabolic function of the enzyme] Genetika. 1981;17(9):1606-17.
  • McNally VA, Gbaj A, Douglas KT, Stratford IJ, Jaffar M, Freeman S, Bryce RA. Identification of a novel class of inhibitor of human and Escherichia coli thymidine phosphorylase by in silico screening Bioorg Med Chem Lett. 2003 Nov 3;13(21):3705-9.
  • Esipov RS, Gurevich AI, Chuvikovsky DV, Chupova LA, Muravyova TI, Miroshnikov AI. Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes Protein Expr Purif. 2002 Feb;24(1):56-60.

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Product Resources:

Manual Manual
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Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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