Ribonucleotide Triphosphate (rGTP) – 100 mM – Research Use Only

Ribonucleotide Triphosphate (rGTP) – 100 mM is a biotechnology-grade guanosine triphosphate (GTP) solution supplied as a 400 µL liquid preparation for research applications involving RNA synthesis, in vitro transcription, enzymatic reactions, and molecular biology workflows. As one of the four essential ribonucleotide triphosphates, rGTP functions as a building block for RNA synthesis and plays a central role in transcription, RNA labeling, enzymatic reactions, and nucleotide metabolism studies. This reagent is especially useful in workflows related to RNA production, CRISPR systems, gene expression studies, sequencing preparation, and biochemical assay development.

Scientific Overview

rGTP (guanosine triphosphate) is one of the four canonical ribonucleotide triphosphates (rNTPs) required for RNA synthesis. It is incorporated into RNA strands during transcription and is essential for a wide range of enzymatic processes involving RNA polymerases, ribozymes, and nucleotide-dependent signaling pathways.

In laboratory workflows, rGTP is commonly used in in vitro transcription systems, RNA labeling reactions, sequencing library preparation, CRISPR-based systems, and enzymatic assays. Its defined concentration and purity make it suitable for applications requiring reproducible RNA synthesis and controlled nucleotide incorporation.

Applications

• In vitro transcription and RNA synthesis workflows
• RNA labeling and nucleotide incorporation studies
• CRISPR and gene expression research systems
• Sequencing library preparation and RNA-based assays
• Biochemical and enzymatic reaction development

Usage & Handling Guidance

Store at −20°C as specified. Thaw on ice prior to use and mix gently. Researchers should avoid repeated freeze–thaw cycles where possible and aliquot if necessary for long-term experimental consistency. Working concentrations should be optimized based on the intended transcription system, enzyme, or assay protocol.

• Preparation: Ready to use; dilute as required for the intended reaction
• Handling: Keep on ice during use and minimize freeze–thaw cycles
• Optimization: Adjust concentration depending on transcription or enzymatic workflow
• Workflow note: Validate performance in the intended RNA synthesis or assay system

What You Get

• 400 µL of 100 mM rGTP solution
• Biotechnology-grade ribonucleotide triphosphate reagent
• Suitable for RNA synthesis and transcription workflows
• Compatible with a wide range of molecular biology applications
• For research use only (RUO)

Why Researchers Choose It

• Essential building block for RNA synthesis and transcription
• Suitable for high-precision molecular biology workflows
• Consistent concentration for reproducible experimental results
• Applicable to CRISPR, sequencing, and RNA assay systems
• Ready-to-use liquid format for convenience

Frequently Asked Questions (FAQ)

• What is rGTP used for in research?
  It is used as a building block in RNA synthesis, in vitro transcription, RNA labeling, and a variety of molecular biology and enzymatic workflows.
• Is rGTP required for RNA synthesis?
  Yes. rGTP is one of the four essential ribonucleotide triphosphates required for RNA polymerase-driven transcription.
• Can this product be used in CRISPR or sequencing workflows?
  Yes. It is commonly relevant in RNA-based CRISPR systems, sequencing preparation, and gene expression studies.
• How should this reagent be stored and handled?
  It should be stored at −20°C and handled on ice during use, with minimal freeze–thaw cycles to maintain stability.
• Is this product intended for diagnostic or therapeutic use?
  No. This product is supplied strictly for research use only and is not intended for diagnostic or therapeutic applications.

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References

• Milligan JF, Groebe DR, Witherell GW, Uhlenbeck OC. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 1987;15(21):8783-8798.
• Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984;12(18):7035-7056.
• He W, et al. Effective Synthesis of High-Integrity mRNA Using In Vitro Transcription. Biomolecules. 2024;14(6):712.
• McCoy LS, et al. Isomorphic Emissive GTP Surrogate Facilitates Initiation and Elongation of In Vitro Transcription Reactions. J Am Chem Soc. 2014;136(28):10176-10182.
• Kanwal F, et al. A Modified In Vitro Transcription Approach to Improve RNA Yield and Integrity. Biotechnol Appl Biochem. 2019;66(5):772-780.
• Varani G. A cap for all occasions. Structure. 1997;5(7):855-858.
• Yang X, et al. In Vitro Transcription Assays and Their Application in Drug Discovery. SLAS Discov. 2016;21(9):881-890.
• Production of Structured RNA Fragments by In Vitro Transcription. Curr Protoc. 2021;1(7):e159.
• Overview of In Vitro Transcription. Thermo Fisher Scientific Technical Resource.
• Strategies for synthesizing in vitro transcribed (IVT) mRNA. New England Biolabs Technical Resource.