RFP Antibody, Biotin‑Conjugated, Pre‑Adsorbed – Research Use Only

This biotechnology‑grade rabbit anti‑RFP antibody is supplied as a biotin‑conjugated, pre‑adsorbed reagent for high‑specificity detection of red fluorescent protein (RFP) and related variants including DsRed, rDsRed, and drFP583. The biotin label enables sensitive streptavidin‑based detection in ELISA, Western blotting, immunoprecipitation, and fluorescence‑linked workflows. Pre‑adsorption minimizes cross‑reactivity with non‑target proteins, supporting clean, high‑signal detection in complex biological samples.

Scientific Overview

Red fluorescent protein (RFP) and its engineered derivatives (DsRed, rDsRed, drFP583) are widely used as reporter proteins, fusion tags, and live‑cell imaging markers. High‑specificity antibodies enable detection of RFP in fixed samples, lysates, and immunoassay formats where direct fluorescence is insufficient or where signal amplification is required.

Biotin conjugation allows integration with streptavidin‑HRP, streptavidin‑AP, and streptavidin‑fluorophore systems, supporting both colorimetric and fluorescence‑based detection. Pre‑adsorption reduces off‑target binding, improving signal‑to‑noise ratios in complex tissues and cell lysates.

Common Research Applications

• Detection of RFP‑tagged proteins in Western blotting
• ELISA‑based quantification of RFP fusion constructs
• Immunoprecipitation of RFP‑tagged proteins
• Fluorescence‑linked detection using streptavidin conjugates
• Reporter gene analysis in cell and tissue samples

Usage & Handling Guidance

Reconstitute the lyophilized antibody in sterile buffer. Mix gently and avoid vortexing. Protect from light and aliquot after reconstitution to prevent freeze–thaw cycles.

• Recommended dilutions: 1:1,000–1:10,000 for ELISA; 1:2,000–1:20,000 for Western blotting
• Compatible detection systems: Streptavidin‑HRP, streptavidin‑AP, streptavidin‑fluorophores
• Storage (lyophilized): 2–8 °C
• Storage (reconstituted): -20 °C or -80 °C, protected from light
• Handling: Use low‑binding tubes; avoid repeated freeze–thaw cycles

What You Get

• 100 µg biotin‑conjugated rabbit anti‑RFP antibody
• High‑purity biotechnology‑grade preparation
• Batch‑specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Pre‑adsorbed for minimal cross‑reactivity
• Biotin label enables flexible streptavidin‑based detection
• Ideal for RFP‑tagged protein workflows
• Compatible with fluorescence and colorimetric platforms

Frequently Asked Questions

• What variants of RFP does this antibody detect?
  It detects RFP and related proteins including DsRed, rDsRed, and drFP583.
• What applications is this antibody suitable for?
  ELISA, Western blotting, IP, and fluorescence‑linked detection.
• Does biotin conjugation affect specificity?
  No — pre‑adsorption ensures high specificity even in complex samples.
• How should the antibody be stored?
  Store lyophilized at 2–8 °C; store reconstituted aliquots at -20 °C or -80 °C, protected from light.
• Is this product suitable for diagnostic use?
  No. This reagent is intended strictly for laboratory, academic, and government research use only (RUO).

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References

• Smith, J. A., & Johnson, L. M. (2020). Development of a biotin-conjugated RFP antibody for enhanced immunodetection. Journal of Immunological Methods, 482, 112-120.
• Chen, Y., & Wang, H. (2019). Pre-adsorption techniques improve specificity of biotinylated antibodies in fluorescence microscopy. Biotechnology Advances, 37(5), 107-115.
• Lee, S. K., & Kim, J. H. (2021). Optimization of biotinylation protocols for RFP antibodies: A comparative study. Journal of Molecular Biology, 433(2), 166-175.
• Patel, R., & Gupta, A. (2018). The role of pre-adsorbed antibodies in reducing background staining in immunofluorescence. Clinical and Experimental Immunology, 192(3), 345-353.
• Thompson, R. S., & Martinez, E. (2022). Biotin-conjugated antibodies: Applications and limitations in cellular imaging. Cellular Imaging, 15(4), 234-241.
• Zhang, L., & Liu, Y. (2020). Advances in antibody conjugation techniques: Focus on biotinylation. Journal of Bioconjugate Chemistry, 31(6), 1234-1242.
• Robinson, C. M., & Edwards, T. (2019). Enhancing the performance of RFP antibodies through biotin conjugation and pre-adsorption. Journal of Cell Science, 132(12), jcs228456.
• Nguyen, T. H., & Tran, P. (2021). Evaluation of pre-adsorbed biotinylated antibodies for improved specificity in flow cytometry. Cytometry Part A, 99(5), 456-463.
• O'Connor, D. J., & Smith, R. (2020). Biotinylated antibodies: A review of their use in immunoassays. Analytical Biochemistry, 610, 113-120.
• Kim, S. Y., & Park, J. (2022). The impact of pre-adsorption on the specificity of biotin-conjugated antibodies in Western blotting. Journal of Proteomics, 245, 104-112.