Red Colored Polystyrene Beads (30 nm) – Catalog #B2013442

Red Colored Polystyrene Beads (30 nm) (Catalog #B2013442) are uniform, brightly fluorescent red-dyed polystyrene nanoparticles supplied as a 1 mL suspension. These 30 nm beads are widely used as traceable markers, calibration standards, and visualization tools in flow cytometry, fluorescence microscopy, immunoassays, cell tracking, and nanotechnology research. Their intense red fluorescence, high stability, and consistent size make them ideal for multiplexed assays, instrument calibration, and studies requiring sub-cellular or nanoscale labeling.

Scientific Overview

These 30 nm red polystyrene beads are internally dyed with a stable red fluorophore, providing bright, photostable fluorescence without significant dye leakage. Their uniform size and surface chemistry make them excellent standards for calibrating flow cytometers, validating optical instruments, and serving as traceable markers in cellular uptake and biodistribution studies. They are commonly used in multiplex assays, phagocytosis research, and as model nanoparticles in drug delivery and nanotoxicology.

Key applications include:

• Flow cytometry calibration and compensation beads
• Fluorescence microscopy and live-cell imaging
• Immunoassay development and lateral flow assays
• Cell tracking and nanoparticle uptake studies
• Instrument performance validation (FCS, confocal, etc.)

Usage & Handling Guidance

Store at 2–8°C. Do not freeze. Gently vortex or pipette up and down before use to ensure homogeneous suspension. Dilute in PBS, culture medium, or assay buffer as needed. Sonication (brief, low power) may be used if minor aggregation occurs after long-term storage.

• Typical working concentration: 10⁵–10⁷ beads/mL (optimize for assay)
• Excitation/Emission: Compatible with standard red/far-red channels (e.g., ~580–620 nm excitation)
• Stability: Stable for months when stored properly; protect from prolonged light exposure

What You Get

• 1 mL of 30 nm Red Colored Polystyrene Beads suspension
• Uniform size distribution and bright red fluorescence
• Biotechnology-grade nanoparticles suitable for sensitive applications
• Ready-to-use format for immediate experimental use
• For research use only (RUO)

Why Researchers Choose It

• Bright, photostable red fluorescence for easy visualization
• Uniform 30 nm size ideal for nanoscale applications and calibration
• Versatile tool for flow cytometry, microscopy, and assay development
• High-quality suspension with minimal aggregation
• Excellent model particles for cellular uptake and biodistribution studies

Frequently Asked Questions (FAQ)

• What is the excitation/emission range?
  Optimized for standard red channels (typically excited ~580–620 nm).
• Can these beads be used in live cells?
  Yes. They are commonly used for phagocytosis, uptake, and tracking studies.
• How should I store the product?
  Store at 2–8°C. Do not freeze. Mix gently before use.
• Are the beads surface-functionalized?
  These are plain dyed polystyrene beads. Carboxyl- or amine-functionalized versions are available separately if conjugation is needed.
• What is the concentration of beads?
  Concentration is optimized for direct experimental use; dilute as needed for your specific protocol.

---

References

• Adams TN, Leonard KM, Minerick AR. Frequency sweep rate dependence on the dielectrophoretic response of polystyrene beads and red blood cells. Biomicrofluidics. 2013 Dec 10;7(6):64114.
• Serhatlioglu M, Asghari M, Tahsin Guler M, Elbuken C. Impedance-based viscoelastic flow cytometry. Electrophoresis. 2019 Mar;40(6):906-913.
• Jung J, Kim K, Yoon J, Park Y. Hyperspectral optical diffraction tomography. Opt Express. 2016 Feb 8;24(3):2006-12.
• Dinga DK, Kasprzycka E, Assunção IP, Winterstein F, Alizade A, Caliskanyürek V, Blödorn D, Winkle J, Kynast U, Lezhnina M. High brightness red emitting polymer beads for immunoassays: Comparison between trifluoroacetylacetonates of Europium. Front Chem. 2023 Apr 20;11:1179247.
• Hashimoto M, Numata M, Yatsushiro S, Ido Y, Tanaka M, Kajimoto K, Kataoka M. Pseudo-Infected Red Blood Cell Beads as Positive Control for Cell Microarray Chip-Based Detection of Plasmodium-Infected RBCs. J Parasitol. 2018 Jun;104(3):283-288.
• Sonawane SL, Asha SK. Blue, green, and orange-red emission from polystyrene microbeads for solid-state white-light and multicolor emission. J Phys Chem B. 2014 Aug 7;118(31):9467-75.
• Baek Y, Lee K, Yoon J, Kim K, Park Y. White-light quantitative phase imaging unit. Opt Express. 2016 May 2;24(9):9308-15.
• Kolber MA, Quinones RR, Henkart PA. Target cell lysis by cytotoxic T lymphocytes redirected by antibody-coated polystyrene beads. J Immunol. 1989 Sep 1;143(5):1461-6.
• Kumar M, Yadav S, Kumar A, Sharma NN, Akhtar J, Singh K. MEMS impedance flow cytometry designs for effective manipulation of micro entities in health care applications. Biosens Bioelectron. 2019 Oct 1;142:111526.