Recombinant Rat Hemopexin Protein – Research Use Only

Recombinant Rat Hemopexin Protein is a high-purity 50.4 kDa glycoprotein supplied as a 50 µg lyophilized powder (Catalog #B2012830, His-tagged). Hemopexin is the primary heme-binding protein in plasma. It binds free heme with extremely high affinity, preventing heme-mediated oxidative damage and facilitating safe heme recycling in the liver via receptor-mediated endocytosis. The recombinant rat version is an excellent tool for studying heme metabolism, oxidative stress, acute-phase responses, and inflammation in rodent models.

Scientific Overview

Hemopexin is a 60 kDa acute-phase glycoprotein that binds free heme with the highest known affinity of any protein. By sequestering heme, it prevents heme-catalyzed formation of reactive oxygen species and lipid peroxidation while delivering heme to hepatocytes for safe catabolism via heme oxygenase-1. In rat models, hemopexin levels increase during inflammation, hemolysis, and tissue injury. The recombinant His-tagged rat hemopexin retains biological activity and is widely used to study heme transport, oxidative stress protection, and acute-phase responses in rodent systems.

This Recombinant Rat Hemopexin Protein is suitable for:

• Heme-binding and transport studies in rat models
• Investigation of oxidative stress and heme-mediated damage
• Acute-phase response and inflammation research
• Development of heme-related biomarker assays
• Studies on hemopexin–receptor interactions and heme recycling

Usage & Handling Guidance

Store the lyophilized powder at -20 °C. Reconstitute in sterile ultrapure water or PBS to the desired concentration. Prepare single-use aliquots to avoid repeated freeze–thaw cycles. The protein remains stable when handled under standard laboratory conditions.

• Recommended applications: Heme-binding assays, oxidative stress studies, and acute-phase response research
• Working concentration: Optimize empirically (commonly 1–50 µg/mL depending on assay)
• Stability: Lyophilized form is stable at -20 °C; reconstituted solutions should be used promptly or stored frozen
• Handling: Use protease-free conditions; keep on ice during dilution if needed

What You Get

• 50 µg Recombinant Rat Hemopexin Protein (His-tagged) as lyophilized powder
• High-purity 50.4 kDa rat hemopexin with native-like heme-binding capacity
• Biotechnology-grade material suitable for biochemical and immunological studies in rat models
• Convenient quantity for multiple experiments and method optimization
• For research use only (RUO)

Why Researchers Choose It

• Authentic recombinant rat hemopexin for species-relevant studies
• Excellent tool for heme-binding, oxidative stress, and acute-phase response research in rodents
• Useful as a control or standard in rat inflammation and injury models
• Stable lyophilized format with reliable lot-to-lot consistency
• Well-characterized protein with extensive literature support in heme metabolism

Frequently Asked Questions (FAQ)

• What is the primary function of hemopexin?
  Hemopexin binds free heme with extremely high affinity, preventing oxidative damage and facilitating safe heme recycling in the liver.
• Why use recombinant rat hemopexin?
  It is ideal for experiments in rat models of inflammation, oxidative stress, and acute-phase responses.
• How should I reconstitute the powder?
  Dissolve in sterile ultrapure water or PBS to the desired concentration. Prepare aliquots and store at -20 °C to avoid repeated freeze–thaw cycles.
• Can this be used in cell-based assays?
  Yes. It can be added to serum-free or low-serum media for heme-related cellular studies.
• How should I store the product?
  Store the lyophilized powder at -20 °C for long-term stability.

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References

• Detsika MG, Lianos EA. Hemopexin Modulates Expression of Complement Regulatory Proteins in Rat Glomeruli. Curr Issues Mol Biol. 2021 Sep 7;43(2):1081-1089.
• Taketani S, Immenschuh S, Go S, Sinclair PR, Stockert RJ, Liem HH, Muller Eberhard U. Hemopexin from four species inhibits the association of heme with cultured hepatoma cells or primary rat hepatocytes exhibiting a small number of species specific hemopexin receptors. Hepatology. 1998 Mar;27(3):808-14.
• Dong B, Cai M, Fang Z, Wei H, Zhu F, Li G, Dong H, Xiong L. Hemopexin induces neuroprotection in the rat subjected to focal cerebral ischemia. BMC Neurosci. 2013 Jun 10;14:58.
• Immenschuh S, Nagae Y, Satoh H, Baumann H, Muller-Eberhard U. The rat and human hemopexin genes contain an identical interleukin-6 response element that is not a target of CAAT enhancer-binding protein isoforms. J Biol Chem. 1994 Apr 29;269(17):12654-61.
• Potter D, Chroneos ZC, Baynes JW, Sinclair PR, Gorman N, Liem HH, Muller-Eberhard U, Thorpe SR. In vivo fate of hemopexin and heme-hemopexin complexes in the rat. Arch Biochem Biophys. 1993 Jan;300(1):98-104.
• Nikkilä H, Gitlin JD, Muller-Eberhard U. Rat hemopexin. Molecular cloning, primary structural characterization, and analysis of gene expression. Biochemistry. 1991 Jan 22;30(3):823-9.
• Madore N, Camborieux L, Bertrand N, Swerts JP. Regulation of hemopexin synthesis in degenerating and regenerating rat sciatic nerve. J Neurochem. 1999 Feb;72(2):708-15.
• Ashouri R, Fangman M, Burris A, Ezenwa MO, Wilkie DJ, Doré S. Critical Role of Hemopexin Mediated Cytoprotection in the Pathophysiology of Sickle Cell Disease. Int J Mol Sci. 2021 Jun 15;22(12):6408.
• Xu M, Tan C, Hu J, Alwahsh SM, Yan J, Hu J, Dai Z, Wang Z, Zhou J, Fan J, Huang X. Expression of hemopexin in acute rejection of rat liver allograft identified by serum proteomic analysis. Shock. 2014 Jul;42(1):65-74.
• Luft S. [Hemopexin]. Acta Haematol Pol. 1971 Jul-Sep;2(3):243-51.