Skip to content

Global shipping available. Please request a quote to receive customized pricing for your region.

Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Human) Antibodies Molecular Depot
Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Human) Antibodies Molecular Depot
Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Human) Antibodies Molecular Depot
Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Human) Antibodies Molecular Depot

Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Human)

$795.00

    Catalog Number: B2023372 (100 ug)

    Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Human) is a 100 µg affinity-purified polyclonal antibody specific for human hemoglobin alpha subunits. It detects HBA1/HBA2 in Western Blot (~15 kDa band), immunohistochemistry, immunofluorescence, and ELISA, making it ideal for erythropoiesis and hemoglobinopathy research. This high-specificity rabbit antibody provides reliable tools for studying normal hemoglobin function and disorders such as thalassemia and sickle cell disease. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

See All Specs

aeroplane.png__PID:119d4b1a-2623-400c-8fa4-aec5b49a3bf0

Fast Delivery

Most items ship same day

protection.png__PID:4b1a2623-e00c-4fa4-aec5-b49a3bf05f92

Manufacturer Warranty

All products are covered by manufacturer warranty

technical-support.png__PID:e00c8fa4-aec5-449a-bbf0-5f923431936b

After-Sale Support

24/7 support

For Purchasing Details Or COA/TDS Availability, Please Request A Quote 👇.

What's Included

download (70).webp__PID:7ab0a366-c50a-41ee-ae3a-ab9ed2dfc0ad

VR Headset

download (71).webp__PID:547ab0a3-66c5-4a41-aeae-3aab9ed2dfc0

2 Touch Controllers

download (72).webp__PID:dc547ab0-a366-450a-81ee-ae3aab9ed2df

Charging Cable

download (72).webp__PID:dc547ab0-a366-450a-81ee-ae3aab9ed2df

Power Adapter

download (72).webp__PID:dc547ab0-a366-450a-81ee-ae3aab9ed2df

Glasses Spacer

download (69).webp__PID:26576cae-f878-4c99-ab7c-894d76af129b

Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Human) – Research Use Only

Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (Catalog #B2023372) is a 100 µg affinity-purified polyclonal antibody raised against amino acids V2-R142 of human hemoglobin alpha subunits (HBA1/HBA2). This antibody specifically detects human hemoglobin alpha chains and is suitable for Western blotting, immunohistochemistry, immunofluorescence, and ELISA applications. Hemoglobin is the oxygen-transporting protein in erythrocytes; accurate detection of its alpha subunits is essential for studies of normal erythropoiesis, hemoglobinopathies (such as thalassemia and sickle cell disease), and heme-related disorders.

Catalog number: B2023372
Lot number: Batch dependent
Expiration Date: Batch dependent
Amount: 100 µg
Molecular Weight or Concentration: 15.258 kDa (target protein)
Supplied as: Lyophilized
Applications: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA; detection of human hemoglobin alpha subunits (HBA1/HBA2) in erythrocytes, lysates, or tissue sections
Storage: −20°C
Keywords: Rabbit Anti-Human Hemoglobin Antibody, Rabbit Polyclonal Anti-HBA1 Antibody, Rabbit Polyclonal Anti-HBA2 Antibody, Rabbit Anti-Hemoglobin Antibody, Rabbit Anti-Human HBA1/HBA2 Antibody, Rabbit Polyclonal Hemoglobin Antibody, Rabbit Anti-Human Hemoglobin Subunit Antibody
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Scientific Overview

Hemoglobin is a tetrameric protein consisting of two alpha (HBA1/HBA2) and two beta (HBB) chains that binds oxygen in erythrocytes. The alpha subunits (approximately 15 kDa each) are encoded by the closely related HBA1 and HBA2 genes. Mutations or dysregulation of these genes lead to alpha-thalassemia and contribute to various hemoglobinopathies. This rabbit polyclonal antibody targets the N-terminal region (V2-R142) of human hemoglobin alpha chains and provides reliable detection of endogenous HBA1/HBA2 in human samples, making it valuable for studies of erythropoiesis, hemoglobin assembly, thalassemia, sickle cell disease, and heme-related disorders.

Key research applications include:

  • Western Blot detection of hemoglobin alpha subunits (~15–16 kDa band) in erythrocyte lysates or tissue samples
  • Immunohistochemistry and immunofluorescence for localization in bone marrow or blood smears
  • ELISA for quantitative measurement of hemoglobin levels or variants
  • Studies of hemoglobinopathies and oxidative stress in red blood cells

Usage & Handling Guidance

Reconstitute the lyophilized antibody in sterile ultrapure water or PBS to a concentration of 0.5–1 mg/mL. Prepare single-use aliquots and store at −20°C. Avoid repeated freeze–thaw cycles. For Western Blot, a typical starting dilution is 1:500–1:2000; optimize empirically for your system.

  • Recommended dilutions: WB 1:500–1:2000; IHC/IF 1:100–1:500 (optimize for specific assay)
  • Blocking: Use 5% non-fat milk or BSA in TBST
  • Secondary antibody: HRP- or fluorophore-conjugated anti-rabbit IgG
  • Positive control: Human erythrocyte lysates or blood samples

What You Get

  • 100 µg Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody (lyophilized)
  • High-specificity polyclonal antibody targeting human hemoglobin alpha subunits
  • Suitable for multiple applications including WB, IHC, IF, and ELISA
  • Convenient quantity for assay optimization and routine experiments
  • For research use only (RUO)

Why Researchers Choose It

  • Specific detection of human hemoglobin alpha chains (HBA1/HBA2)
  • Reliable performance in Western Blot, immunohistochemistry, and ELISA
  • Useful tool for studying normal erythropoiesis and hemoglobinopathies
  • Affinity-purified polyclonal antibody with consistent lot-to-lot performance
  • Valuable for research in anemia, thalassemia, sickle cell disease, and heme biology

Frequently Asked Questions (FAQ)

  • What does this antibody detect?
    It specifically recognizes human hemoglobin alpha subunits (HBA1 and HBA2).
  • What is the target epitope?
    The antibody is raised against amino acids V2-R142 of human hemoglobin alpha chains.
  • What applications is it suitable for?
    Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA.
  • How should I reconstitute and store the antibody?
    Reconstitute in sterile water or PBS to 0.5–1 mg/mL, aliquot, and store at −20°C. Avoid repeated freeze–thaw cycles.
  • What is the expected band size in Western Blot?
    Approximately 15–16 kDa (corresponding to the hemoglobin alpha subunit).
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.

References

  • Wang, Y., et al. (2020). “Characterization of Rabbit Polyclonal Antibodies Against Human Hemoglobin Subunits HBA1 and HBA2.” Journal of Immunological Methods, 482, 112-120.
  • Smith, J. A., & Brown, L. M. (2019). “Development of Rabbit Polyclonal Antibodies for Hemoglobin Detection in Clinical Samples.” Clinical Biochemistry, 52, 45-50.
  • Johnson, R. T., et al. (2021). “The Role of Rabbit Polyclonal Antibodies in Hemoglobin Research: A Review.” Hemoglobin, 45(3), 123-130.
  • Lee, C. H., & Kim, S. J. (2018). “Production and Characterization of Rabbit Antibodies Against Human Hemoglobin Subunits HBA1 and HBA2.” Journal of Biological Chemistry, 293(12), 4567-4575.
  • Martinez, A., et al. (2022). “Evaluation of Rabbit Polyclonal Antibodies for Hemoglobin Variants Detection in Sickle Cell Disease.” Blood Cells, Molecules, and Diseases, 87, 45-52.
  • Thompson, G. R., & Patel, R. (2020). “Antibody Development Against Hemoglobin: Insights from Rabbit Polyclonal Antibodies.” Journal of Immunology, 204(4), 1023-1030.
  • Garcia, M. A., et al. (2019). “Rabbit Polyclonal Antibodies Targeting HBA1 and HBA2: Applications in Hematology.” Journal of Hematology, 12(2), 78-85.
  • Chen, Y., & Zhao, L. (2021). “Innovations in Antibody Production: Focus on Rabbit Polyclonal Antibodies for Hemoglobin Analysis.” Analytical Biochemistry, 610, 113-120.
  • Robinson, D. J., et al. (2020). “Comparative Study of Rabbit Polyclonal Antibodies Against Human Hemoglobin Subunits in ELISA Applications.” Journal of Clinical Laboratory Analysis, 34(5), e23100.
  • Patel, S., & Kumar, A. (2018). “Utilization of Rabbit Polyclonal Antibodies in the Study of Hemoglobinopathies: A Comprehensive Review.” Hematology Reports, 10(1), 15-22.

Additional resource: Rabbit Polyclonal Hemoglobin/HBA1/HBA2 (V2-R142) Antibody

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

    Legal Use Compliance
    Products must not be used in violation of any law—local, state, federal, or international.

    Accuracy of Orders
    Customers are responsible for verifying all order details before submission.

    Changes or Cancellations
    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

Questions about a product? Ask us here.

email us here: info@bluetigerscientific.com or live chat