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Pyruvate Kinase from Rabbit Muscle Proteins Molecular Depot
Pyruvate Kinase from Rabbit Muscle Proteins Molecular Depot
Pyruvate Kinase from Rabbit Muscle Proteins Molecular Depot
Pyruvate Kinase from Rabbit Muscle Proteins Molecular Depot

Pyruvate Kinase from Rabbit Muscle

$907.00

    Catalog Number: B2013451 (10 kU)

    Pyruvate Kinase from Rabbit Muscle is native Pyruvate Kinase purified from the rabbit muscle. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. For in vitro research use only. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Pyruvate Kinase from Rabbit Muscle

Catalog # B2013451
Lot # Batch dependent
Expiration Batch dependent
Amount 10 kU
Activity 250 U/mg protein
Supplied as Lyophilized powder
Storage –20°C
Keywords Pyruvate Kinase
Grade Biotechnology grade; ultrapure water (>18 MΩ·cm); filtered through 0.22 µm

Pyruvate Kinase (PK) from rabbit muscle is a key glycolytic enzyme that catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, generating ATP in the final step of glycolysis. This highly purified preparation is supplied as a lyophilized powder with an activity of 250 U/mg protein and a total of 10,000 units per vial. It is ideal for use in metabolic pathway analysis, enzymatic assays, and studies of energy metabolism and allosteric regulation.

Rabbit muscle PK is a well-characterized isoform with known structural and kinetic properties, making it a reliable model for biochemical and structural studies. The enzyme is stable at –20°C and should be reconstituted in sterile buffer for optimal performance. All components are prepared using Type I ultrapure water and filtered through 0.22 µm membranes to ensure consistency and reproducibility across batches.

Key benefits:

  • High-purity PK enzyme from rabbit muscle
  • Validated activity (250 U/mg protein)
  • Lyophilized format for long-term stability
  • Supports glycolysis, energy metabolism, and structural biology research
  • Ideal for kinetic assays and metal ion interaction studies

Indication for Use:
This product is for Research Use Only (RUO). It is not intended for human or animal use, diagnostic procedures, or therapeutic applications.

References:

  • Bazaes S, Bosch M, Schäfer HJ, Eyzaguirre J. Photoaffinity labeling of pyruvate kinase from rabbit muscle Arch Biol Med Exp. 1988 Jun;21(1):123-7.
  • Larsen TM, Laughlin LT, Holden HM, Rayment I, Reed GH. Structure of rabbit muscle pyruvate_kinase complexed with Mn2+, K+, and pyruvate Biochemistry. 1994 May 24;33(20):6301-9.
  • Ueda S, Sakasegawa SI. Pyruvate kinase from Geobacillus stearothermophilus displays an unusual preference for Mn(2+) in a cycling reaction Anal Biochem. 2019 Apr 1;570:27-31.
  • Hinrichs MV, Eyzaguirre J. Affinity labeling of rabbit muscle pyruvate kinase with dialdehyde-ADP Biochim Biophys Acta. 1982 Jun 4;704(2):177-85.
  • Boehme C, Bieber F, Linnemann J, Breitling R, Lorkowski S, Reissmann S. Chemical and enzymatic characterization of recombinant rabbit muscle pyruvate_kinase Biol Chem. 2013 May;394(5):695-701.
  • Laughlin LT, Reed GH. The monovalent cation requirement of rabbit muscle pyruvate_kinase is eliminated by substitution of lysine for glutamate 117 Arch Biochem Biophys. 1997 Dec 15;348(2):262-7.
  • Bygrave FL. Studies on the interaction of metal ions with pyruvate_kinase from Ehrlich ascites-tumour cells and from rabbit muscle Biochem J. 1966 Nov;101(2):488-94.
  • Erhard K, Davis RC. Phosphoenolpyruvate hydrolase activity of rabbit muscle pyruvate_kinase J Biol Chem. 1975 Aug 10;250(15):945-50.
  • Strumilo S, Tylicki A. [Different properties of pyruvate kinase from rabbit and hare muscles] Zh Evol Biokhim Fiziol. 2015 Mar-Apr;51(2):103-7.
  • Schering B, Eigenbrodt E, Linder D, Schoner W. Purification and properties of pyruvate kinase type M2 from rat lung Biochim Biophys Acta. 1982 Aug 6;717(2):337-47.

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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