Pvu I Restriction Enzyme – Research Use Only

Pvu I Restriction Enzyme is a high-quality site-specific endonuclease that recognizes the DNA sequence 5'-CGAT↓CG-3' and cleaves between the T and C residues, producing blunt ends. Supplied as 250 units in solution (Catalog #B2016569), this biotechnology-grade enzyme is an essential tool for molecular cloning, DNA mapping, restriction fragment length polymorphism (RFLP) analysis, and preparation of DNA fragments for ligation or sequencing.

Scientific Overview

Pvu I is a type II restriction endonuclease that specifically recognizes the palindromic sequence CGATCG and cleaves it to produce blunt-ended DNA fragments. This enzyme is widely used in molecular biology for precise DNA cutting, subcloning, plasmid verification, and construction of recombinant DNA molecules. Its ability to generate blunt ends makes it particularly useful when compatible ends are required for ligation with other blunt-cut fragments or when directional cloning strategies are employed.

This Pvu I Restriction Enzyme is suitable for:

• Restriction digestion of plasmid DNA and genomic DNA
• Molecular cloning and vector construction
• Restriction fragment length polymorphism (RFLP) analysis
• Verification of recombinant plasmids and insert orientation
• Preparation of blunt-ended DNA fragments for ligation

Usage & Handling Guidance

Store at −20 °C. Thaw on ice before use and keep the enzyme cold during setup. Perform digestions in the recommended buffer (typically supplied or compatible 10× restriction buffer) at 37 °C for 1–2 hours or overnight, depending on the amount of DNA and desired completeness. Use 5–10 units per µg of DNA as a starting point and optimize as needed.

• Recommended applications: Standard restriction digestion, cloning, and plasmid analysis
• Reaction conditions: 37 °C in appropriate 1× restriction buffer; incubate 1–16 hours
• Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour at 37 °C
• Handling: Avoid repeated freeze–thaw cycles; keep on ice during reaction setup

What You Get

• 250 units of Pvu I Restriction Enzyme in solution
• High-specificity endonuclease that generates blunt ends at CGAT↓CG
• Biotechnology-grade enzyme suitable for routine molecular cloning and analysis
• Convenient quantity for multiple experiments and method optimization
• For research use only (RUO)

Why Researchers Choose It

• Reliable and specific cleavage at the CGATCG recognition site with blunt ends
• Essential tool for molecular cloning, subcloning, and plasmid verification
• High activity and consistent performance in standard restriction buffers
• Biotechnology-grade quality ensures minimal star activity and high fidelity
• Well-suited for RFLP analysis and preparation of DNA fragments for ligation

Frequently Asked Questions (FAQ)

• What is the recognition sequence for Pvu I?
  Pvu I recognizes 5'-CGAT↓CG-3' and produces blunt ends.
• What is the optimal reaction temperature?
  37 °C is the standard incubation temperature for Pvu I.
• How many units should I use per reaction?
  Typically 5–10 units per µg of DNA. For complete digestion of supercoiled plasmids, higher amounts or longer incubation may be required.
• Can Pvu I be heat-inactivated?
  Yes. Heat inactivation is usually performed at 65–80 °C for 20 minutes, but confirm with your specific protocol or buffer.
• Is this enzyme compatible with other restriction enzymes?
  Yes. It works well in most standard restriction buffers and can be used in double digests when buffer compatibility is confirmed.

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References

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• Morimoto R, Rabinowitz M. Physical mapping of the Xba I, Hinc II, Bgl II, Xho I, Sst I, and Pvu II restriction endonuclease cleavage fragments of mitochondrial DNA of S. cerevisiae. Mol Gen Genet. 1979 Feb 16;170(1):11-23.
• Zhang ZL, Qin YJ, He JW, Huang QR, Hu YQ, Li M, Liu YJ, Zhang H. [Association of Xba I, Pvu II, and Bst U I polymorphisms of estrogen receptor-alpha gene with bone mass in men]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2005 Aug;22(4):447-9.
• Zhang ZL, Zhang CZ, Li Y, Zhao ZH, Yang SE. Association between ERα gene Pvu II polymorphism and breast cancer susceptibility: A meta-analysis. Medicine (Baltimore). 2018 Apr;97(17):e0317.
• Zhang J, Bai H, Liu XH, Fan P, Liu R, He GL. [The Pvu II and Xba I polymorphisms in the estrogen receptor alpha gene in Chinese patients with severe preeclampsia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Feb;26(1):40-4.
• Huang H, Ba Y, Cui L, Cheng X, Zhu J, Zhang Y, Yan P, Zhu C, Kilfoy B, Zhang Y. COL1A2 gene polymorphisms (Pvu II and Rsa I), serum calciotropic hormone levels, and dental fluorosis. Community Dent Oral Epidemiol. 2008 Dec;36(6):517-22.
• Yazdi MM, Jamalaldini MH, Sobhan MR, Jafari M, Mazaheri M, Zare-Shehneh M, Neamatzadeh H. Association of ESRα Gene Pvu II T>C, XbaI A>G and BtgI G>A Polymorphisms with Knee Osteoarthritis Susceptibility: A Systematic Review and Meta-Analysis Based on 22 Case-Control Studies. Arch Bone Jt Surg. 2017 Nov;5(6):351-362.
• Koh JM, Kim DJ, Hong JS, Park JY, Lee KU, Kim SY, Kim GS. Estrogen receptor alpha gene polymorphisms Pvu II and Xba I influence association between leptin receptor gene polymorphism (Gln223Arg) and bone mineral density in young men. Eur J Endocrinol. 2002 Dec;147(6):777-83.
• Gylling H, Kontula K, Miettinen TA. Cholesterol absorption and metabolism and LDL kinetics in healthy men with different apoprotein E phenotypes and apoprotein B Xba I and LDL receptor Pvu II genotypes. Arterioscler Thromb Vasc Biol. 1995 Feb;15(2):208-13.
• Song YM, Shin J, Ko H, Yoo JE, Cho IY, Shin DW, Cho B, Lee JE, Hwang J, Lee J. Factors associated with pneumococcal vaccination uptake by cancer survivors. J Cancer Surviv. 2021 Apr;15(2):234-243.