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Protein Molecular Weight Standard 15 - 600 kDa Proteins Molecular Depot
Protein Molecular Weight Standard 15 - 600 kDa Proteins Molecular Depot
Protein Molecular Weight Standard 15 - 600 kDa Proteins Molecular Depot
Protein Molecular Weight Standard 15 - 600 kDa Proteins Molecular Depot

Protein Molecular Weight Standard 15 - 600 kDa

$647.00

    Catalog Number: B2012432 (30 mg)

    The Protein Molecular Weight Standard (15–600 kDa) is a biotechnology‑grade mixture of highly purified proteins formulated for accurate molecular weight estimation in SDS‑PAGE, Tricine‑PAGE, and size‑exclusion chromatography workflows. This broad‑range ladder provides sharp, well‑resolved bands across low‑, mid‑, and high‑molecular‑weight regions, supporting routine protein characterization, verification of recombinant protein expression, and electrophoretic method development.Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Protein Molecular Weight Standard (15–600 kDa) – Research Use Only

This Protein Molecular Weight Standard provides a broad 15–600 kDa range suitable for SDS‑PAGE, Tricine‑PAGE, and size‑exclusion chromatography workflows. The ladder contains a defined mixture of highly purified proteins formulated for sharp, consistent banding and reliable molecular weight estimation across denaturing and non‑denaturing electrophoretic systems. Its wide dynamic range supports routine protein characterization, verification of recombinant protein expression, and method development in proteomics and structural biology.

Catalog number: B2012432
Lot number: Batch dependent
Expiration Date: Batch dependent
Amount: 30 mg
Molecular Weight Range: 15–600 kDa
Supplied as: Lyophilized
Applications: Protein electrophoresis, molecular weight estimation, SEC calibration
Storage: −20 °C
Keywords: Protein Standard Mix 15–600 kDa
Grade: Biotechnology grade. Prepared with Type I ultrapure water (>18 MΩ·cm) and sterile‑filtered (0.22 µm).

Scientific Overview

Molecular weight standards are essential controls for electrophoretic protein separation. This ladder spans low‑molecular‑weight peptides through large multimeric proteins, enabling accurate size estimation across a wide range of sample types. The formulation is optimized for clear band resolution in SDS‑PAGE and Tricine‑PAGE, and is compatible with Coomassie, silver staining, and fluorescent detection methods. The broad range also supports calibration of size‑exclusion chromatography columns and validation of protein oligomerization states.

Common Research Applications

  • SDS‑PAGE and Tricine‑PAGE molecular weight estimation
  • Verification of recombinant protein expression and purity
  • Calibration of size‑exclusion chromatography (SEC) columns
  • Assessment of protein oligomerization or degradation
  • Proteomics method development and QC
  • Benchmarking electrophoretic separation performance

Usage & Handling Guidance

Reconstitute the lyophilized standard in ultrapure water or electrophoresis sample buffer. Mix gently to avoid foaming. Aliquot after reconstitution to prevent freeze–thaw degradation. Compatible with reducing and non‑reducing conditions.

  • Reconstitution: Water or sample buffer
  • Compatibility: SDS‑PAGE, Tricine‑PAGE, SEC
  • Storage: −20 °C (lyophilized or aliquoted)
  • Avoid: repeated freeze–thaw cycles

What You Get

  • 30 mg broad‑range protein molecular weight standard
  • Lyophilized, biotechnology‑grade formulation
  • Suitable for electrophoresis and SEC calibration
  • For research use only (RUO)

Frequently Asked Questions

  • What staining methods are compatible with this ladder?
    The standard is compatible with Coomassie, silver stain, and most fluorescent protein stains.
  • Can this ladder be used for native PAGE?
    Yes. While optimized for SDS‑PAGE, the proteins can also serve as approximate markers in native systems.
  • Is this suitable for SEC column calibration?
    Yes. The 15–600 kDa range is appropriate for calibrating analytical SEC columns.
  • Can I obtain a COA or TDS?
    Yes. Documentation availability is batch dependent. Please contact us to request COA/TDS for the lot being shipped.

References

  • Matsumoto H, Haniu H, Komori N. Determination of Protein Molecular Weights on SDS-PAGE. Methods Mol Biol. 2019.
  • Haider SR, Reid HJ, Sharp BL. Tricine-SDS-PAGE. Methods Mol Biol. 2019.
  • Some D et al. SEC-MALS characterization of proteins. J Vis Exp. 2019.
  • Irvine GB. Molecular size determination by gel filtration. Curr Protoc Cell Biol. 2001.
  • Santilli RT et al. Penn State Protein Ladder system. Sci Rep. 2021.
  • Haider SR et al. Tricine-SDS-PAGE. Methods Mol Biol. 2012.
  • Krutz NL et al. Protein reactivity toward lysine residues. Toxicol Sci. 2021.
  • Castellví A et al. BioSAXS for low‑MW proteins. Acta Crystallogr D. 2020.
  • Wheaton S, Gordon R. Optical nanotweezers for protein MW characterization. Analyst. 2015.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

    Legal Use Compliance
    Products must not be used in violation of any law—local, state, federal, or international.

    Accuracy of Orders
    Customers are responsible for verifying all order details before submission.

    Changes or Cancellations
    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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