Protein A Turbo – Proximity Biotinylation Enzyme (RUO)

Protein A Turbo is a biotechnology‑grade proximity biotinylation enzyme designed for rapid and efficient labeling of proteins in spatial proximity to antibody‑bound targets. By fusing Protein A to TurboID, this reagent enables “off‑the‑shelf” proximity labeling without the need for genetic engineering of the target protein. When combined with a primary antibody, Protein A Turbo recruits TurboID to the protein of interest, allowing biotinylation of nearby interactors for downstream enrichment and proteomic analysis. This approach supports interaction proteomics, chromatin‑associated protein mapping, and antibody‑guided proximity labeling workflows.

Scientific Overview

Protein A Turbo enables antibody‑directed proximity biotinylation by coupling the high‑affinity IgG‑binding properties of Protein A with the rapid labeling kinetics of TurboID. Upon addition of biotin, TurboID catalyzes the formation of reactive biotin‑AMP, which covalently labels lysine residues on nearby proteins. This allows researchers to map protein–protein interactions, chromatin‑associated complexes, and spatially localized proteomes without genetic fusion of TurboID to the target protein. The method is compatible with fixed or permeabilized cells, isolated nuclei, and biochemical preparations.

Common Research Applications

• Antibody‑guided proximity labeling using TurboID
• Interaction proteomics and protein complex mapping
• Chromatin‑associated protein identification
• Spatial proteomics and subcellular protein profiling
• Antibody‑based enrichment of protein microenvironments
• Proximity labeling in cells, nuclei, or biochemical extracts

Usage & Handling Guidance

Store at -80 °C. Thaw on ice and aliquot immediately to avoid freeze–thaw cycles. Use low‑binding tubes and maintain cold conditions during handling. For optimal performance, combine Protein A Turbo with a validated primary antibody and add biotin to initiate labeling.

• Storage: -80 °C
• Handling: Aliquot upon receipt; avoid freeze–thaw cycles
• Compatibility: TurboID workflows, proteomics, proximity labeling assays

What You Get

• 50 µg Protein A Turbo (Proximity Biotinylation Enzyme)
• High‑purity biotechnology‑grade preparation
• Batch‑specific documentation available upon request
• For research use only (RUO)

Frequently Asked Questions

• How does Protein A Turbo work?
  Protein A binds to the Fc region of IgG antibodies, positioning TurboID near the antibody‑bound target. Upon biotin addition, TurboID biotinylates nearby proteins for downstream enrichment.
• Do I need to genetically modify my target protein?
  No. Protein A Turbo enables proximity labeling using only a primary antibody, eliminating the need for genetic fusion constructs.
• What types of samples are compatible?
  Protein A Turbo can be used in cells, permeabilized cells, isolated nuclei, and biochemical extracts, depending on the workflow.
• Can this reagent be used for chromatin‑associated protein mapping?
  Yes. Antibody‑guided TurboID labeling is widely used for chromatin‑associated proteomics.
• How should the enzyme be stored?
  Store at -80 °C and aliquot to avoid freeze–thaw cycles.
• Is this reagent suitable for diagnostic or therapeutic use?
  No. This product is intended strictly for in vitro research use only (RUO).
• Can I request a COA or TDS?
  Yes. COA/TDS availability is batch dependent. Contact us with the catalog and lot number.

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References

• Santos‑Barriopedro I, van Mierlo G, Vermeulen M. Off‑the‑shelf proximity biotinylation using ProtA‑TurboID. Nat Protoc. 2023.
• Santos‑Barriopedro I, van Mierlo G, Vermeulen M. Off‑the‑shelf proximity biotinylation for interaction proteomics. Nat Commun. 2021.
• Cho KF et al. Proximity labeling with TurboID and split‑TurboID. Nat Protoc. 2020.
• Cho KF et al. Split‑TurboID enables contact‑dependent labeling. PNAS. 2020.
• May DG et al. Comparative application of BioID and TurboID. Cells. 2020.
• Kido K et al. AirID proximity labeling enzyme. eLife. 2020.