Polystyrene Particles Conjugated to FITC (50 nm) – Research Use Only

Polystyrene Particles Conjugated to FITC (50 nm) are uniform 50 nm polystyrene nanoparticles covalently labeled with fluorescein isothiocyanate (FITC). Supplied as a 1% suspension in 0.5 mL volume, these fluorescent nanobeads provide bright green fluorescence for use as tracers, calibration standards, and model systems in fluorescence-based assays, cellular uptake studies, and nanotechnology research.

Scientific Overview

Fluorescent polystyrene nanoparticles are widely used as traceable model systems in nanomedicine, cell biology, and bioimaging. These 50 nm FITC-conjugated polystyrene particles combine a well-defined size with bright, stable green fluorescence (excitation ~495 nm, emission ~520 nm). They serve as excellent tools for studying cellular uptake mechanisms, intracellular trafficking, nanoparticle–cell interactions, and as size/calibration standards in fluorescence-based techniques.

These Polystyrene Particles Conjugated to FITC (50 nm) are suitable for:

• Cellular uptake and endocytosis studies in mammalian cells
• Fluorescence microscopy and live-cell imaging
• Flow cytometry and nanoparticle tracking analysis
• Development of fluorescent calibration beads and standards
• Investigation of size-dependent biological effects of nanoparticles
• Bioimaging and biodistribution studies

Usage & Handling Guidance

Store the 1% suspension at 2–8 °C. Do not freeze. Gently vortex or pipette the suspension to ensure uniform dispersion before each use. Sonication (brief, low power) may be required after prolonged storage to fully resuspend the particles.

• Recommended applications: Fluorescence microscopy, flow cytometry, cellular uptake studies, and nanoparticle tracking
• Working concentration: Dilute to 0.01–0.1% solids (optimize for your specific assay and instrument)
• Excitation / Emission: FITC channel (Excitation ~495 nm, Emission ~520 nm)
• Handling: Protect from light; use in sterile conditions for cell-based experiments

What You Get

• 0.5 mL of 50 nm Polystyrene Particles Conjugated to FITC as a 1% suspension
• Uniform size distribution with stable covalent FITC labeling
• Bright green fluorescence suitable for sensitive imaging and flow cytometry
• Biotechnology-grade fluorescent nanoparticles
• For research use only (RUO)

Why Researchers Choose It

• Well-defined 50 nm size with bright, stable FITC fluorescence
• Excellent model system for studying nanoparticle–cell interactions and uptake
• Useful as calibration standards in fluorescence microscopy and flow cytometry
• High surface area suitable for further functionalization or tracking experiments
• High-purity 1% suspension manufactured under controlled conditions

Frequently Asked Questions (FAQ)

• What are the fluorescence properties?
  FITC-labeled: Excitation ~495 nm, Emission ~520 nm (standard green/FITC channel).
• How should I store and prepare the suspension?
  Store at 2–8 °C. Do not freeze. Gently vortex or sonicate briefly before use to ensure uniform dispersion.
• What working concentration is recommended?
  Dilute to 0.01–0.1% solids depending on the assay. Optimize for your imaging or flow cytometry setup.
• Are these particles suitable for live-cell imaging?
  Yes. They are commonly used for studying endocytosis and intracellular trafficking in live cells.
• Can the particles be further functionalized?
  The polystyrene surface allows additional conjugation if needed, though they are already FITC-labeled for direct fluorescence tracking.

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