Polystyrene Particles Conjugated to FITC (5 µm) – Catalog #B2013345

Polystyrene Particles Conjugated to FITC (5 µm) (Catalog #B2013345) are brightly fluorescent green microspheres supplied as a 1 mL 1% aqueous suspension. These 5 µm particles are covalently labeled with fluorescein isothiocyanate (FITC), providing strong green fluorescence (excitation ~495 nm, emission ~519 nm) that is fully compatible with standard FITC/GFP filter sets. They are excellent tools for fluorescence microscopy, flow cytometry calibration, phagocytosis studies, cell–particle interaction research, agglutination assays, and as traceable markers in diagnostic and nanomedicine applications.

Scientific Overview

These 5 µm FITC-conjugated polystyrene particles combine a uniform microsphere core with bright, stable green fluorescence from covalently attached FITC. The larger 5 µm size makes them particularly suitable for direct visualization under standard fluorescence microscopes, phagocytosis assays with macrophages or neutrophils, and as calibration standards in flow cytometry. The covalent labeling ensures minimal dye leakage, enabling reliable long-term tracking and quantitative studies.

Key applications include:

• Fluorescence microscopy and live-cell imaging
• Phagocytosis and immune cell interaction studies
• Flow cytometry calibration and compensation
• Agglutination and particle-based immunoassays
• Cell tracking and biodistribution experiments

Usage & Handling Guidance

Store at 2–8°C. Do not freeze. Gently vortex or pipette up and down before use to ensure homogeneous suspension. Dilute in PBS, culture medium, or assay buffer as required. Brief low-power sonication can be used if minor aggregation occurs after long-term storage.

• Typical working concentration: 10⁵–10⁷ beads/mL (optimize for assay)
• Excitation/Emission: ~495 nm / ~519 nm (standard FITC/GFP filter sets)
• Stability: Stable for months when stored refrigerated; protect from prolonged light exposure

What You Get

• 1 mL of 5 µm Polystyrene Particles Conjugated to FITC (1% suspension)
• Bright, photostable green fluorescence with covalent FITC labeling
• Uniform particle size for reproducible experimental results
• Ready-to-use aqueous suspension
• For research use only (RUO)

Why Researchers Choose It

• Bright FITC fluorescence compatible with standard laboratory equipment
• 5 µm size ideal for microscopy, phagocytosis, and flow cytometry applications
• Stable covalent conjugation minimizes dye leakage
• Versatile tool for cell biology, immunology, and diagnostic assay development
• High-quality biotechnology-grade particles with consistent performance

Frequently Asked Questions (FAQ)

• What are the excitation and emission wavelengths?
  Approximately 495 nm excitation and 519 nm emission (standard FITC channel).
• Can these particles be used in phagocytosis studies?
  Yes. The 5 µm size is optimal for macrophage and neutrophil phagocytosis assays.
• How should I store the suspension?
  Store at 2–8°C. Do not freeze. Mix gently before each use.
• Are the particles surface-functionalized?
  These are FITC-conjugated polystyrene particles. Additional surface chemistry options are available if further conjugation is needed.
• What is the concentration?
  1% (w/v) suspension. Dilute according to your experimental needs.

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References

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