Polystyrene Nanoparticles Conjugated to FITC (20 nm) – Catalog #B2013281

Polystyrene Nanoparticles Conjugated to FITC (20 nm) (Catalog #B2013281) are brightly fluorescent green nanoparticles supplied as a 1 mL 1% aqueous suspension. These 20 nm particles are covalently labeled with fluorescein isothiocyanate (FITC), offering excellent brightness and compatibility with standard FITC/GFP filter sets (excitation ~495 nm, emission ~519 nm). They are ideal for fluorescence imaging, flow cytometry, cellular uptake studies, biodistribution tracking, and as traceable markers in nanomedicine and assay development.

Scientific Overview

These 20 nm FITC-conjugated polystyrene nanoparticles combine the stable, inert polystyrene core with the bright green fluorescence of fluorescein isothiocyanate. FITC provides strong emission in the green channel with excellent compatibility for standard microscopy and flow cytometry setups. The small size and covalent labeling make them excellent tools for studying nanoparticle–cell interactions, intracellular trafficking, vascular imaging, and as size standards in fluorescence-based techniques.

Key applications include:

• Live-cell fluorescence imaging and confocal microscopy
• Flow cytometry and nanoparticle uptake studies
• Cellular internalization and endosomal trafficking research
• Biodistribution and pharmacokinetics of nanoparticles
• Multiplexed assays and instrument calibration

Usage & Handling Guidance

Store at 2–8°C. Do not freeze. Gently vortex or pipette up and down before use to ensure uniform suspension. Dilute in PBS, culture medium, or assay buffer as needed. Brief low-power sonication can help if minor aggregation occurs after storage.

• Typical working concentration: 10⁵–10⁷ particles/mL (optimize for your assay)
• Excitation/Emission: ~495 nm / ~519 nm (standard FITC/GFP filter sets)
• Stability: Stable for months when stored properly; protect from prolonged light exposure

What You Get

• 1 mL of 20 nm Polystyrene Nanoparticles Conjugated to FITC (1% suspension)
• Bright, photostable green fluorescence
• Uniform particle size for reproducible results
• Ready-to-use aqueous suspension
• For research use only (RUO)

Why Researchers Choose It

• Classic FITC fluorescence compatible with most laboratory equipment
• Small 20 nm size ideal for cellular uptake and nanomedicine studies
• Stable covalent conjugation minimizes dye leakage
• Versatile tool for imaging, flow cytometry, and tracking applications
• High-quality biotechnology-grade particles with consistent performance

Frequently Asked Questions (FAQ)

• What are the excitation and emission wavelengths?
  Approximately 495 nm excitation and 519 nm emission (standard FITC channel).
• Can these particles be used in live cells?
  Yes. They are commonly used for phagocytosis, endocytosis, and intracellular tracking studies.
• How should I store the suspension?
  Store at 2–8°C. Do not freeze. Mix gently before each use.
• Are the particles surface-functionalized?
  These are FITC-conjugated polystyrene nanoparticles. Additional surface chemistry options are available if further conjugation is required.
• What is the concentration?
  1% (w/v) suspension. Dilute according to experimental needs.

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