Polystyrene Beads with Amine Groups (20 nm) – Catalog #B2013396

Polystyrene Beads with Amine Groups (20 nm) (Catalog #B2013396) are uniform 20 nm polystyrene nanoparticles bearing surface amine (-NH₂) functional groups, supplied as a 1 mL aqueous suspension. The primary amine groups enable straightforward covalent conjugation to carboxyl-containing molecules, proteins, antibodies, dyes, or other ligands using standard EDC/NHS chemistry. These aminated beads are excellent tools for creating custom functionalized nanoparticles, targeted drug delivery systems, diagnostic assays, and surface chemistry studies.

Scientific Overview

These 20 nm amine-functionalized polystyrene beads provide reactive primary amine groups on the surface for easy covalent attachment of biomolecules via EDC/NHS coupling or other amine-reactive chemistries. The small size combined with high surface area makes them ideal for creating targeted nanoconjugates, fluorescent probes, drug-delivery vehicles, and diagnostic reagents. They are widely used in nanomedicine, immunoassay development, cell labeling, and fundamental studies of nanoparticle–cell interactions.

Key applications include:

• Covalent conjugation of antibodies, proteins, peptides, or dyes
• Development of targeted nanoparticles for drug delivery
• Immunoassays and lateral flow diagnostics
• Cellular uptake and intracellular trafficking studies
• Fluorescent labeling and imaging probes

Usage & Handling Guidance

Store at 2–8°C. Do not freeze. Gently vortex or pipette up and down before use to ensure uniform suspension. For conjugation, wash beads if necessary and follow standard EDC/NHS protocols. After conjugation, blocking with glycine or ethanolamine is recommended to quench unreacted groups.

• Typical working concentration: 0.1–1% (w/v) for conjugation reactions
• Conjugation chemistry: EDC/NHS for carboxyl-containing ligands
• Stability: Stable for months when stored refrigerated; protect from contamination

What You Get

• 1 mL of 20 nm Polystyrene Beads with Amine Groups (aqueous suspension)
• Reactive surface amine groups for covalent bioconjugation
• Uniform particle size and high surface area
• Ready for direct use in coupling reactions
• For research use only (RUO)

Why Researchers Choose It

• Ready-to-conjugate amine surface for easy attachment of biomolecules
• Small 20 nm size ideal for nanomedicine and cellular uptake studies
• Stable, high-quality polystyrene core with low non-specific binding
• Versatile platform for creating custom fluorescent or targeted nanoparticles
• Convenient suspension format for immediate experimental use

Frequently Asked Questions (FAQ)

• What chemistry can be used for conjugation?
  Standard EDC/NHS coupling to carboxyl groups is most common. Other amine-reactive reagents (e.g., NHS-esters) can also be used.
• Can these beads be used in live cells?
  Yes. They are suitable for cellular uptake, trafficking, and nanomedicine studies after conjugation.
• How should I store the product?
  Store at 2–8°C. Do not freeze. Mix gently before use.
• What is the concentration?
  The suspension is typically used as supplied or diluted according to your conjugation protocol.
• Are the beads fluorescent?
  No. These are plain aminated polystyrene beads. Fluorescent versions (e.g., FITC, Cy5) are available separately.

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